Use of slow-releasing hydrogen sulfide organic donor ADT-OH in the preparation of drugs for treating nervous system diseases

Patent metadata

Field	Value
Publication number	US-12478608-B2
Application number	US-202117912638-A
Country	US
Kind code	B2
Filing date	Oct 9, 2021
Priority date	Sep 7, 2021
Publication date	Nov 25, 2025
Grant date	Nov 25, 2025

Abstract

The present invention relates to use of slow-releasing hydrogen sulfide organic donor ADT-OH drugs in the differentiation of neural precursor cells. ADT-OH can induce more directed differentiation of neural precursor cells into neurons and oligodendrocytes and less differentiation into astrocytes, promote the axon growth of neurons, and reduce the death of neural precursor cells, thereby providing a direction for the transplantation of neural precursor cells to repair damaged nerves. ADT-OH will likely become a new drug target for the clinical treatment of nervous system diseases.

First claim

What is claimed is:

1 . A method for in-vitro differentiation of neural precursor cells, comprising:
providing a slow-releasing hydrogen sulfide organic donor ADT-OH; culturing the neural precursor cells in a neural precursor cell culture medium containing the ADT-OH for 1-3 days, a concentration of the ADT-OH in the neural precursor cell culture medium containing ADT-OH being 70-90 μmol/L, the neural precursor cell culture medium being DMEM-F12 medium containing B27, bEGF and FGF; and inductively culturing the neural precursor cells in a differentiation medium containing the slow-releasing hydrogen sulfide organic donor ADT-OH for 5-8 days, a concentration of the ADT-OH in the differentiation medium containing the ADT-OH being 70-90 μmol/L, the differentiation medium being DMEM-F12 medium containing FBS and B27.

2 . The method according to claim 1 , wherein the slow-releasing hydrogen sulfide organic donor ADT-OH is used to induce the differentiation of neural precursor cells into neurons.

3 . The method according to claim 1 , wherein the slow-releasing hydrogen sulfide organic donor ADT-OH is used to promote the axonal growth of neurons.

4 . The method according to claim 1 , wherein the slow-releasing hydrogen sulfide organic donor ADT-OH is used to induce the differentiation of neural precursor cells into oligodendrocytes.

5 . The method according to claim 1 , wherein the slow-releasing hydrogen sulfide organic donor ADT-OH is used to inhibit the differentiation of neural precursor cells into astrocytes.

6 . The method according to claim 1 , wherein the neural precursor cells are neural precursor cells in the subventricular zone in the embryonic stage.

7 . A method for inducing the differentiation of neural precursor cells, comprising steps of:
culturing the neural precursor cells in a neural precursor cell culture medium containing ADT-OH for 1-3 days, and then inductively culturing in a differentiation medium containing ADT-OH for 5-8 days; wherein the concentration of ADT-OH in the neural precursor cell culture medium containing ADT-OH is 70-90 μmol/L, the concentration of ADT-OH in the differentiation medium containing ADT-OH is 70-90 μmol/L, the neural precursor cell culture medium is DMEM-F12 medium containing B27, bEGF and FGF, and the differentiation medium is DMEM-F12 medium containing FBS and B27.

8 . The method according to claim 7 , wherein a tissue is taken from the subventricular zone in the embryonic stage, trypsinized, and terminated with the neural precursor cell culture medium, to obtain free neural precursor cells; and the pellet is collected, added to the neural precursor cell culture medium, then inoculated in a cell culture plate and cultured for 3-4 days, to obtain the neural precursor cells.

Assignees

• Univ Soochow

Inventors

• Ma Yanxia
• Wei Shanwen
• Zou Mingming
• Ni Li
• Zhou Liyu
• Li Di
• Yang Yanwei

Classifications

• C12N5/0622

  Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells · CPC title

• A61K35/30

  Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title

• A61P25/00 Primary

  Drugs for disorders of the nervous system · CPC title

• C12N2501/999

  Small molecules not provided for elsewhere · CPC title

• C12N2501/119

  Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10 · CPC title