Promoter and use thereof

Patent metadata

Field	Value
Publication number	US-12473558-B2
Application number	US-202117911133-A
Country	US
Kind code	B2
Filing date	Nov 12, 2021
Priority date	May 20, 2021
Publication date	Nov 18, 2025
Grant date	Nov 18, 2025

Abstract

The present application relates to a novel promoter and a method for producing target materials using the same. More specifically, the present application relates to a novel polynucleotide having promoter activity, a gene expression cassette, and a host cell comprising the same, and a method for producing target materials using the microorganism.

First claim

The invention claimed is:

1 . A polynucleotide having promoter activity, in which the nucleotides at positions 218, 219, 220, 221, 222, 225, and 227 in the polynucleotide sequence of SEQ ID NO: 1 are substituted with other nucleotides.

2 . The polynucleotide of claim 1 , wherein the polynucleotide comprises a substitution of cytosine (C), the nucleotide at position 218, with thymine (T), a substitution of cytosine (C), the nucleotide at position 219, with guanine (G), a substitution of adenine (A), the nucleotide at position 220, with thymine (T), a substitution of adenine (A), the nucleotide at position 221, with guanine (G), a substitution of thymine (T), the nucleotide at position 222, with guanine (G), a substitution of adenine (A), the nucleotide at position 225, with thymine (T), and a substitution of cytosine (C), the nucleotide at position 227, with adenine (A), in the polynucleotide sequence of SEQ ID NO: 1.

3 . The polynucleotide of claim 1 , wherein the polynucleotide is composed of a nucleotide sequence of SEQ ID NO: 2.

4 . The polynucleotide of claim 1 , wherein the polynucleotide is operably linked to a gene encoding a target protein.

5 . A gene expression cassette, comprising: the polynucleotide claim 1 ; and a gene encoding a target protein which is operably linked to the polynucleotide.

6 . The gene expression cassette of claim 5 , wherein the target protein is transketolase.

7 . A host cell, comprising: the polynucleotide of claim 1 ; and a gene encoding a target protein which is operably linked to the polynucleotide.

8 . The host cell of claim 7 , wherein the target protein is transketolase.

9 . The host cell of claim 7 , wherein the host cell is a microorganism of the genus Corynebacterium.

10 . The host cell of claim 9 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum.

11 . A method for producing a target material, comprising: culturing the host cell of claim 7 in a medium; and recovering the target material from the medium.

12 . The method of claim 11 , wherein the target material is an amino acid.

13 . The method of claim 11 , wherein the target material is at least one selected from the group consisting of lysine, threonine, O-acetyl homoserine, isoleucine, tryptophan, tyrosine, and phenylalanine.

14 . The gene expression cassette of claim 5 , wherein the polynucleotide comprises a substitution of cytosine (C), the nucleotide at position 218, with thymine (T), a substitution of cytosine (C), the nucleotide at position 219, with guanine (G), a substitution of adenine (A), the nucleotide at position 220, with thymine (T), a substitution of adenine (A), the nucleotide at position 221, with guanine (G), a substitution of thymine (T), the nucleotide at position 222, with guanine (G), a substitution of adenine (A), the nucleotide at position 225, with thymine (T), and a substitution of cytosine (C), the nucleotide at position 227, with adenine (A), in the polynucleotide sequence of SEQ ID NO: 1.

15 . The gene expression cassette of claim 5 , wherein the polynucleotide is composed of a nucleotide sequence of SEQ ID NO: 2.

16 . The host cell of claim 7 , wherein the polynucleotide comprises a substitution of cytosine (C), the nucleotide at position 218, with thymine (T), a substitution of cytosine (C), the nucleotide at position 219, with guanine (G), a substitution of adenine (A), the nucleotide at position 220, with thymine (T), a substitution of adenine (A), the nucleotide at position 221, with guanine (G), a substitution of thymine (T), the nucleotide at position 222, with guanine (G), a substitution of adenine (A), the nucleotide at position 225, with thymine (T), and a substitution of cytosine (C), the nucleotide at position 227, with adenine (A), in the polynucleotide sequence of SEQ ID NO: 1.

17 . The host cell of claim 7 , wherein the polynucleotide is composed of a nucleotide sequence of SEQ ID NO: 2.

Assignees

• Cj Cheiljedang Corp

Inventors

• Kwon Nara
• Park Sojung
• Jung Moo Young
• Kim Kyungrim
• Kim Heeyeong
• Lee Jaemin
• Kim Hyun Ah

Classifications

• C12Y202/01001

  Transketolase (2.2.1.1) · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title

• C12N9/1022 Primary

  transferring aldehyde or ketonic groups (2.2) · CPC title

• C12R2001/15

  Corynebacterium · CPC title