Compositions comprising textured patterns and methods of using the same

Patent metadata

Field	Value
Publication number	US-12472286-B2
Application number	US-202218059288-A
Country	US
Kind code	B2
Filing date	Nov 28, 2022
Priority date	Jan 28, 2016
Publication date	Nov 18, 2025
Grant date	Nov 18, 2025

Abstract

The invention provides method of guiding unidirectional movement of a cell comprising exposing the cell to a surface comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements for a time period sufficient to bias actin polymerization within the cell. The invention also provides methods of inducing directional movement of a cell, and methods of harnessing an actin wave within a cell. In addition, the invention provides compositions comprising a contact side, wherein at least a portion of the contact side comprises a cell contact portion comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements, and methods of inducing healing of a wound of a subject comprising contacting the wound with the composition.

First claim

The invention claimed is:

1 . A method of guiding unidirectional movement of a first cell in a sample comprising different cell types, the method comprising:
exposing the sample to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the first cell; biasing movement of the first cell in a first direction along the asymmetric topographical elements; and biasing movement of a second cell in a second direction along the asymmetric topographical elements, wherein the first and the second cells are different cell types and the first and second directions are different; wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface.

2 . The method of claim 1 , wherein the topographical elements are protrusions aligned in a set of evenly spaced rows across at least a portion of the surface.

3 . The method of claim 1 , wherein the three-dimensional pattern of asymmetric topographical elements comprise at least one or a plurality of saw-toothed, columnar, ridge or pyramidal shaped elements.

4 . The method of claim 1 , wherein the topographical elements are saw-tooth protrusions aligned in a set of evenly spaced rows across at least a portion of the surface.

5 . The method of claim 4 , wherein the saw-tooth protrusions are from about 0.4 to about 2.5 microns in height, from about 0.8 to about 8.0 microns in length, from about 0.1 to about 0.8 microns in width and from about 0.8 and to about 2.2 microns in spacing between consecutive protrusions along the path or between rows.

6 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that are from about 0.1 to about 3.0 microns in height.

7 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that are from about 0.05 to about 1.00 micron in width.

8 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that are from about 0.3 to about 20.0 microns in length.

9 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that have a repeat distance from about 0.3 to about 25.0 microns.

10 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that have a pitch of from about 0.2 to about 6.0 microns.

11 . The method of claim 3 , wherein the asymmetric topographical elements are saw tooth protrusions that have an offset distance from about 0 to about 12.5 microns.

12 . The method of claim 1 , wherein the method comprises movement of the cell across a distance of at least about 5 microns.

13 . A method of inducing directional movement of a cell in a sample comprising different cell types, the method comprising:
contacting the sample to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the cell; selectively guiding movement of only cells of a first cell type in a first direction along the asymmetric topographical elements; wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface.

14 . The method of claim 13 wherein the movement is across a distance of at least about 5 microns.

15 . A method of inducing healing of a wound of a subject, comprising:
contacting the wound with a contact side of a composition, at least a portion of the contact side having a cell contact portion with a three-dimensional pattern of one or a plurality of asymmetric topographical elements, the topographical elements being from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the cell contact portion; biasing movement of a first cell type in a first direction along the asymmetric topographical elements; and biasing movement of a second cell type in a second direction different than the first direction along the topographical elements; wherein biasing movement of the first cell type in the first direction along the asymmetric topographical elements includes:
contacting the wound with the composition for a time period sufficient to bias actin polymerization within a plurality of cells of the first cell type at, adjacent to, or proximate to the wound such that the cells unidirectionally move toward or away from a direction defined by the sidedness of the asymmetric uppermost points of the topographical elements.

16 . The method of claim 15 wherein the composition comprises a surface configured for contacting a wound wherein the surface comprises a three-dimensional pattern comprising one or a plurality of offset, parallel paths of asymmetric saw tooth-shaped protrusions, wherein the protrusions have a repeat distance from about 0.3 to about 25.0 microns, a pitch of from about 0.2 to about 6.0 microns, and an offset distance from about 0 to about 12.5 microns.

17 . The method of claim 1 , wherein the first cell type is one of a neutrophil, fibroblast, or epithelial cell.

18 . The method of claim 13 , wherein the first cell type is one of a neutrophil, fibroblast, or epithelial cell.

19 . A method of guiding movement of a cell, comprising:
providing a surface with a three-dimensional pattern of one or a plurality of asymmetric topographical elements, the asymmetric topographical elements having a first side with a first side length and a second side with a second side length shorter than the first side length; contacting the cell with the surface including the asymmetric topographical elements; biasing movement of a first cell type along the first side of the asymmetric topographical elements; and biasing movement of a second cell type along the second side of the asymmetric topographical elements; wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved, or circumferential paths on the surface.

Assignees

• Univ Maryland
• Univ Maryland

Inventors

• Losert Wolfgang
• Fourkas John T
• Driscoll Meghan
• Bates Ii Floyd
• Sun Xiaoyu
• Guven Can
• Parker Joshua

Classifications

• A61F13/0243 Primary

  characterised by the properties of the skin contacting layer, e.g. air-vapor permeability · CPC title

• A61L27/50 Primary

  Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} · CPC title