Molecular entrapment via homopolymer self-assembly

Patent metadata

Field	Value
Publication number	US-12472152-B2
Application number	US-202318472901-A
Country	US
Kind code	B2
Filing date	Sep 22, 2023
Priority date	Jul 29, 2019
Publication date	Nov 18, 2025
Grant date	Nov 18, 2025

Abstract

The present invention provides novel nanostructures comprising solution of PPSU 20 . Methods of preparing the novel PPSU nanostructures, and applications of such nanostructures are also provided.

First claim

The invention claimed is:

1 . A method of loading a poly(propylene sulfone) (PPSU) homopolymer nanostructure with a cargo, the method comprising incubating the nanostructure with the cargo in a buffer for up to 5 minutes, thereby loading the nanostructure with the cargo.

2 . The method of claim 1 , wherein the incubating comprises vortexing the nanostructure and the cargo.

3 . The method of claim 1 , wherein the buffer comprises phosphate buffered saline (PBS).

4 . The method of claim 1 , further comprising washing the nanostructure loaded with the cargo at least three times.

5 . The method of claim 4 , further comprising storing the nanostructure in the buffer at about 4° C.

6 . The method of claim 1 , wherein the nanostructure is a vesicle-like nanostructure.

7 . The method of claim 6 , wherein the vesicle-like nanostructure has a diameter of less than 240 nm.

8 . The method of claim 1 , wherein the nanostructure is a bundle-like or micellar nanostructure.

9 . The method of claim 1 , wherein the nanostructure consists of PPSU homopolymers.

10 . The method of claim 1 , wherein each of the PPSU homopolymers comprises twenty sulfones.

11 . The method of claim 1 , wherein the cargo is selected from a drug, a protein, a DNA, an RNA, and a siRNA.

12 . The method of claim 11 , wherein the cargo is a protein.

13 . The method of claim 1 further comprising producing the nanostructure from a solution comprising the PPSU homopolymers and a first solvent; and washing the produced nanostructure to remove the first solvent prior to incubating the nanostructure with the cargo.

14 . The method of claim 13 , wherein the cargo has an activity in the first solvent and an activity in the buffer and wherein the activity in the first solvent is lower than the activity in the buffer.

15 . The method of claim 14 , wherein the cargo is selected from a drug, a protein, a DNA, an RNA, and a siRNA.

16 . The method of claim 14 , wherein the cargo is a protein.

17 . The method of claim 14 , wherein the first solvent comprises DMSO.

18 . The method of claim 17 , wherein the buffer comprises phosphate buffered saline (PBS).

Assignees

• Univ Northwestern

Inventors

• Scott Evan A
• Du Fanfan
• Qiao Baofu
• De La Cruz Monica Olvera

Classifications

• C08G75/24 Primary

  Polysulfonates · CPC title

• A61K9/107

  Emulsions {; Emulsion preconcentrates; Micelles (composition of emulsions A61K47/00)} · CPC title

• A61K9/5192

  Processes · CPC title

• A61K9/5146 Primary

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides · CPC title