Body fluid iron level panel analyzer

Patent metadata

Field	Value
Publication number	US-12467933-B2
Application number	US-202017616562-A
Country	US
Kind code	B2
Filing date	Jun 8, 2020
Priority date	Jun 7, 2019
Publication date	Nov 11, 2025
Grant date	Nov 11, 2025

Abstract

Provided herein are systems and methods of assessing a concentration of iron in a body fluid sample, such as whole blood. Systems include a highly stable, fast reacting, and accurate sensing area of a sensor for contacting with a body fluid sample, wherein upon contact, the body fluid sample causes a color change to the sensor that correlates with the concentration of iron in the body fluid sample. The disclosed systems and methods generate one or more signal outputs of light intensity data, from which the concentration of iron in the body fluid sample is determined.

First claim

What is claimed is:

1 . A system for measuring the concentration of iron and total iron binding capacity in a body fluid sample, the system comprising:
a sensor strip comprising:
a first layer comprising a screening film operable to receive the body fluid sample;
a second layer adjacent to the first layer, the second layer comprising a first reagent for reducing iron (III) to iron (II) in the body fluid sample;
a third layer adjacent to the second layer, the third layer comprising a sensing area comprising a second reagent for chelating iron (II) to form a chromogen complex and a reference area without a reagent modification, wherein formation of the chromogen complex causes a color change to the sensor strip that correlates with the concentration of iron in the body fluid sample;
a fourth layer operable as a detection sink;
a fifth layer saturated with iron; and
a sixth layer saturated with magnesium carbonate for measuring total iron binding capacity; and
a processor configured for use in conjunction with the sensor strip, including instructions which, when executed, cause the processor to:
receive light intensity data comprising light intensities from the sensor strip after the body fluid sample is placed on the sensor strip and causes a color change to the sensor strip;
extract red-green-blue (RGB) component values or red spectrum light intensities from the light intensity data of the sensor strip;
calculate the absorbance of the RGB component values or red spectrum light intensities for the sensor strip; and
calculate iron concentration in the body fluid sample in the sensor strip from the absorbance of the RGB component values or red spectrum light intensity for the sensor strip.

2 . The system of claim 1 , wherein the first reagent comprises a reducing agent, an acid, a chelating agent, or combinations thereof, and the second reagent comprises a Ferene.

3 . The system of claim 2 , wherein the reducing agent is ascorbic acid, the acid is citric acid, and the chelating agent is thiourea.

4 . The system of claim 1 , further comprising a device, comprising a window for a light detector, a recess configured for receiving the sensor strip, and a plurality of LED lights.

Assignees

• Forzani Erica
• Jackemeyer David
• Serhan Michael
• Univ Arizona State

Inventors

• Forzani Erica
• Jackemeyer David
• Serhan Michael

Classifications

• G01N2201/062

  LED's · CPC title

• G01N2021/7759

  Dipstick; Test strip · CPC title

• G01N33/84

  involving inorganic compounds or pH · CPC title

• G01N33/80

  involving blood groups or blood types {or red blood cells (white blood cells G01N33/56972)} · CPC title

• G01N33/525

  Multi-layer analytical elements · CPC title