Fluorescence resonance energy transfer (FRET) nanoprobe and preparation method and use thereof

Patent metadata

Field	Value
Publication number	US-12467927-B2
Application number	US-202217905226-A
Country	US
Kind code	B2
Filing date	May 25, 2022
Priority date	Mar 18, 2022
Publication date	Nov 11, 2025
Grant date	Nov 11, 2025

Abstract

The present disclosure provides a fluorescence resonance energy transfer (FRET) nanoprobe and a preparation method and use thereof, and belongs to the technical field of targeted nanomaterials. The FRET nanoprobe includes a loading component and an encapsulating component that encapsulates the loading component, where the loading component includes a carrier and DOX coated in the carrier, and the carrier is a carboxyl-modified hollow mesoporous silica nanoparticle (HMSN); and the encapsulating component includes an RVRR peptide and PAMAM/TPE. In the present disclosure, based on condensation properties of a carboxyl group of the carboxyl-modified HMSN, the RVRR peptide and the small-molecule polymer PAMAM/TPE are encapsulated on an outer surface of the HMSN by a reaction of the RVRR peptide that is specifically responsive to Furin and an amino group modified on a surface of the PAMAM/TPE with the carboxyl group on the surface of the HMSN. Therefore, the DOX is effectively encapsulated.

First claim

What is claimed is:

1 . A fluorescence resonance energy transfer (FRET) nanoprobe, comprising a loading component and an encapsulating component that encapsulates the loading component, wherein the loading component comprises a carrier and DOX coated in the carrier, and the carrier is a carboxyl-modified hollow mesoporous silica nanoparticle (HMSN); and the encapsulating component comprises an RVRR peptide and PAMAM/TPE.

2 . The FRET nanoprobe according to claim 1 , wherein the carboxyl-modified HMSN and the DOX have a mass ratio of (1-2):(1-2).

3 . A method for preparing the FRET nanoprobe according to claim 1 , comprising the following steps:
1) Mixing a carboxyl-modified HMSN solution and a DOX solution to obtain an HMSN/DOX solution; and 2) mixing the HMSN/DOX solution, the RVRR peptide, and the PAMAM/TPE to obtain the FRET nanoprobe.

4 . The method according to claim 3 , wherein in step 1) and step 2), the mixing is conducted independently at 20° C. to 30° C. for 20 h to 24 h in the dark.

5 . The method according to claim 3 , wherein the carboxyl-modified HMSN and the DOX have a mass ratio of (1-2):(1-2).

Assignees

• Univ Qilu Technology

Inventors

• Guo Yingshu
• Zheng Xiaofei

Classifications

• G01N33/533

  with fluorescent label · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• A61K49/0054

  Macromolecular compounds, i.e. oligomers, polymers, dendrimers · CPC title

• A61K49/0021

  the fluorescent group being a small organic molecule · CPC title

• A61P35/00

  Antineoplastic agents · CPC title