Methods and compositions for synthesis of two-photon cleavable phosphoramidite molecules for oligonucleotide conjugation
US-11938187-B2 · Mar 26, 2024 · US
Mutant aminoacyl tRNA synthetase
US12467044B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12467044-B2 |
| Application number | US-202217818919-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 10, 2022 |
| Priority date | Feb 13, 2017 |
| Publication date | Nov 11, 2025 |
| Grant date | Nov 11, 2025 |
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Abstract
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Provided herein are novel materials and methods for site-specific incorporation of phosphotyrosines into proteins. The novel methods of the invention encompass the use of a novel aminoacyl tRNA synthetase capable of charging compatible tRNAs with a phosphotyrosine precursor. The phosphotyrosine precursor is then incorporated, site-specifically, into a protein at sites where phosphotyrosine residues are desired. The phosphotyrosine precursors are subsequently treated to convert them into phosphotyrosine residues, yielding proteins with phosphotyrosines at selected sites. The scope of the invention encompasses novel aminoacyl tRNA synthetases, novel phosphotyrosine precursors, and methods of using these materials to create site-specific phosphorylated tyrosine residues in a protein.
First claim
Opening claim text (preview).
What is claimed is: 1 . A mutant aminoacyl tRNA synthetase, comprising (a) a mutant Methanosarcina mazei pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 2 and comprising serine at amino acid position 302, methionine at amino acid position 309, leucine at amino acid position 322, alanine at amino acid position 346, glycine at amino acid position 348, and threonine at amino acid position 417; or (b) a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 3 and comprising serine at amino acid position 267, methionine at amino acid position 274, leucine at amino acid position 287, alanine at amino acid position 311, glycine at amino acid position 313, and threonine at amino acid position 382. 2 . The mutant aminoacyl tRNA synthetase of claim 1 , wherein the mutant aminoacyl tRNA synthetase is the mutant Methanosarcina mazei pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 2 and comprising serine at amino acid position 302, methionine at amino acid position 309, leucine at amino acid position 322, alanine at amino acid position 346, glycine at amino acid position 348, and threonine at amino acid position 417. 3 . The mutant aminoacyl tRNA synthetase of claim 1 , wherein the mutant aminoacyl tRNA synthetase is the mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase having a sequence with at least 95% identity to SEQ ID NO: 3 and comprising serine at amino acid position 267, methionine at amino acid position 274, leucine at amino acid position 287, alanine at amino acid position 311, glycine at amino acid position 313, and threonine at amino acid position 382. 4 . The mutant aminoacyl tRNA synthetase of claim 1 , wherein the mutant aminoacyl tRNA synthetase is expressed in a cell. 5 . The mutant aminoacyl tRNA synthetase of claim 4 , wherein the cell is any of a bacterial, yeast, plant, or mammalian cell.
Assignees
Inventors
Classifications
Pyrrolysine-tRNAPyl ligase (6.1.1.26) · CPC title
Phosphoramidates · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
Genes encoding for enzymes or proenzymes · CPC title
Ligases (6) · CPC title
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- What does patent US12467044B2 cover?
- Provided herein are novel materials and methods for site-specific incorporation of phosphotyrosines into proteins. The novel methods of the invention encompass the use of a novel aminoacyl tRNA synthetase capable of charging compatible tRNAs with a phosphotyrosine precursor. The phosphotyrosine precursor is then incorporated, site-specifically, into a protein at sites where phosphotyrosine resi…
- Who is the assignee on this patent?
- Univ California
- What technology area does this patent fall under?
- Primary CPC classification C07F9/242. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 11 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).