Fructose-4-epimerase and method of producing tagatose using the same
US-11753646-B2 · Sep 12, 2023 · US
Fructose-4-epimerase and method for preparing tagatose using same
US12460236B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12460236-B2 |
| Application number | US-202017635157-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 13, 2020 |
| Priority date | Aug 14, 2019 |
| Publication date | Nov 4, 2025 |
| Grant date | Nov 4, 2025 |
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Abstract
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The present application relates to a fructose-4-epimerase variant exhibiting tagatose conversion activity and a method for preparing tagatose using the same.
First claim
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The invention claimed is: 1 . A fructose-4-epimerase variant, comprising substitutions of amino acid residues corresponding to positions 97, 124, 367, and 390 from a N-terminus of a fructose-4-epimerase comprising the amino acid sequence of SEQ ID NO: 1 with other amino acids. 2 . The fructose-4-epimerase variant according to claim 1 , wherein the other amino acids are selected from the group consisting of a non-polar amino acid, a polar amino acid, and a basic amino acid. 3 . The fructose-4-epimerase variant according to claim 2 , wherein the non-polar amino acid, polar amino acid, and basic amino acid are selected from the group consisting of tyrosine (Y), tryptophan (W), valine (V), and arginine (R). 4 . The fructose-4-epimerase variant according to claim 1 , wherein the variant further comprises a substitution of an amino acid corresponding to position 158 or 321 with another amino acid. 5 . The fructose-4-epimerase variant according to claim 4 , wherein the another amino acid is selected from the group consisting of glutamine (Q), histidine (H), alanine (A), valine (V), glycine (G), threonine (T), glutamic acid (E), methionine (M), arginine (R), leucine (L), asparagine (N), isoleucine (I), and lysine (K). 6 . The fructose-4-epimerase variant according to claim 1 , wherein the variant further comprises a substitution of amino acid residues corresponding to one or more positions selected from the group consisting of position 210, position 239, and position 318 with other amino acids. 7 . A polynucleotide encoding the fructose-4-epimerase variant according to claim 1 . 8 . A vector comprising the polynucleotide according to claim 7 . 9 . A microorganism comprising the fructose-4-epimerase variant according to claim 1 or a vector comprising a polynucleotide encoding the fructose-4-epimerase variant according to claim 1 . 10 . A composition for tagatose production, comprising the fructose-4-epimerase variant according to claim 1 ; a microorganism comprising the fructose-4-epimerase variant; or a culture of the microorganism. 11 . The composition for tagatose production according to claim 10 , wherein the composition further comprises fructose. 12 . A method for preparing tagatose, the method comprising bringing the fructose-4-epimerase variant according to claim 1 ; a microorganism comprising the fructose-4-epimerase variant; or a culture of the microorganism into contact with fructose and converting fructose into tagatose.
Assignees
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Classifications
- C12Y501/03Primary
acting on carbohydrates and derivatives (5.1.3) · CPC title
produced by the action of an isomerase, e.g. fructose · CPC title
- C12N9/90Primary
Isomerases (5.) · CPC title
- C12P19/02Primary
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
Genes encoding for enzymes or proenzymes · CPC title
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- What does patent US12460236B2 cover?
- The present application relates to a fructose-4-epimerase variant exhibiting tagatose conversion activity and a method for preparing tagatose using the same.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12Y501/03. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 04 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).