Scaffold material for cell culture and cell culture container

Patent metadata

Field	Value
Publication number	US-12460167-B2
Application number	US-202017612451-A
Country	US
Kind code	B2
Filing date	Jul 31, 2020
Priority date	Aug 2, 2019
Publication date	Nov 4, 2025
Grant date	Nov 4, 2025

Abstract

Provided is a cell culture scaffold capable of allowing cell masses to be formed easily and efficiently. A cell culture scaffold according to the present invention includes a substrate; and a protrusion formed by patterning in a pattern of a dot or a line on the substrate, wherein the protrusion contains a synthetic resin, the synthetic resin contains at least a polyvinyl alcohol derivative or a poly(meth)acrylic resin, the protrusion has cell adhesion, and a portion of the protrusion located at the protrusion center position in plan view has an average height of 10 nm or more and 10 μm or less.

First claim

The invention claimed is:

1 . A cell culture scaffold comprising:
a substrate; and a protrusion formed by patterning in a pattern of a dot or a line on the substrate, the protrusion containing a synthetic resin, the synthetic resin containing a peptide-conjugated polyvinyl acetal resin, the peptide-conjugated polyvinyl acetal resin having a polyvinyl acetal resin moiety, a linker moiety, and a peptide moiety, the protrusion having cell adhesion, and a portion of the protrusion located at the protrusion center position in plan view having an average height of 10 nm or more and 10 μm or less.

2 . The cell culture scaffold according to claim 1 , wherein
the ratio of a protein adsorption amount of the protrusion with respect to that of a portion where no protrusion is provided (a protein adsorption amount of the protrusion/a protein adsorption amount of the portion where no protrusion is provided) is 2 or more.

3 . The cell culture scaffold according to claim 1 , wherein
the protrusion is formed by patterning in a pattern of a dot on the substrate, and the protrusion has an average bottom area of 0.005 mm 2 or more and 5 mm 2 or less.

4 . The cell culture scaffold according to claim 1 , wherein the protrusion has a wall portion at a peripheral edge part.

5 . The cell culture scaffold according to claim 1 , wherein the protrusion has an elastic modulus at 25° C. of 1 GPa or more, the elastic modulus being measured at the protrusion center position in plan view by a nanoindentation method.

6 . The cell culture scaffold according to claim 1 , wherein the synthetic resin contains a Brønsted basic group in an amount of 0.2 mol % or more and 20 mol % or less in a constitutional unit.

7 . The cell culture scaffold according to claim 1 , wherein the protrusion has a water swelling rate of 50% or less.

8 . The cell culture scaffold according to claim 1 , wherein the protrusion has the peptide moiety on a surface thereof.

9 . A cell culture container comprising the cell culture scaffold according to claim 1 in at least a part of a cell culture region.

10 . The cell culture scaffold according to claim 1 , wherein the linker moiety is formed by a linker, and the linker comprises a carboxylic acid having a polymerizable unsaturated group.

Assignees

• Sekisui Chemical Co Ltd

Inventors

• Arai Yuuhei
• Inui Nobuhiko
• Haneda Satoshi
• Iguchi Hiroki

Classifications

• C12M25/14 Primary

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M23/12 Primary

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title