Reagent for mass spectrometry

Patent metadata

Field	Value
Publication number	US-12459905-B2
Application number	US-202117154501-A
Country	US
Kind code	B2
Filing date	Jan 21, 2021
Priority date	Jul 24, 2018
Publication date	Nov 4, 2025
Grant date	Nov 4, 2025

Abstract

The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

First claim

The invention claimed is:

1 . A compound of formula A selected from the group consisting of

2 . A composition comprising the compound of claim 1 .

3 . A kit comprising the compound of claim 1 or the composition of claim 2 .

4 . A covalent adduct comprising an analyte molecule and the compound of claim 1 covalently linked to each other.

5 . A method of using a compound of Formula A for the mass spectrometric determination of an analyte molecule, wherein the compound of formula A is selected from the group consisting of

said method comprising the steps:
(a) reacting the analyte molecule with a compound of formula A as defined in claim 1 , whereby a covalent adduct of the analyte molecule and the compound of formula A is formed, and
(b) subjecting the adduct from step (a) to a mass spectrometric analysis,
wherein the mass spectrometric analysis comprises:

(i) subjecting an ion of the adduct to a first stage of mass spectrometric analysis, whereby the ion of the adduct is characterised according to its mass/charge (m/z) ratio,
(ii) causing fragmentation of the adduct ion, whereby a first neutral entity is released and a daughter ion of the adduct is generated, wherein the daughter ion of the adduct differs in its m/z ratio from the adduction, and
(iii) subjecting the daughter ion of the adduct to a second stage of mass spectrometric analysis, whereby the daughter ion of the adduct is characterized according to its m/z ratio, and/or
wherein (ii) may further comprise alternative fragmentation of the adduct ion, whereby a second neutral entity different from the first neutral entity is released and a second daughter ion of the adduct is generated, and

wherein (iii) may further comprise subjecting the first and second daughter ions of the adduct to a second stage of mass spectrometric analysis, whereby the first and second daughter ions of the adduct are characterised according to their m/z ratios.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Heindl Dieter
• Josel Hans-Peter
• Kobold Uwe
• Seidel Christoph
• Rempt Martin
• Leinenbach Andreas
• Prencipe Giuseppe
• Baecher Silvia
• Loibl Simon Ferdinand
• Geiermann Anna-Skrollan
• Milic Jelena
• Hahn Robert
• Pirkl Nicole

Classifications

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• C07D249/06

  with aryl radicals directly attached to ring atoms · CPC title

• G01N30/7233

  interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title

• C07D257/04 Primary

  Five-membered rings · CPC title