Automated bioreactor system, system for automatically implementing protocol for decellularizing organ, and waste decontamination system

Patent metadata

Field	Value
Publication number	US-12458015-B2
Application number	US-202418680636-A
Country	US
Kind code	B2
Filing date	May 31, 2024
Priority date	Sep 2, 2014
Publication date	Nov 4, 2025
Grant date	Nov 4, 2025

Abstract

An automated bioreactor system for decellularizing an organ includes a main chamber for containing the organ. The system further includes a reagent chamber containing a liquid phase reagent. A reagent conduit delivers the liquid phase reagent to the main chamber, and a perfusion conduit delivers the reagent from the reagent outlet in the main chamber into the organ. A perfusion pump drives the flow of the reagent. A perfusion pressure sensor detects a pressure of the flowing reagent. A control system controls the perfusion pump to drive the flow of the reagent based on a received input representative of a desired pressure and a received input of the detected pressure. The control system may automatically perform all of the steps of a decellularization protocol based on sensor input. An automated waste decontamination system may also be provided.

First claim

What is claimed is:

1 . A waste decontamination system for a bioreactor, comprising:
a main chamber configured to contain an organ for perfusing; a waste chamber configured to receive waste fluid from the main chamber; a waste conduit configured to deliver the waste fluid from the main chamber to the waste chamber; a decontamination fluid chamber configured to store decontamination fluid; a decontamination conduit configured to introduce the decontamination fluid from the decontamination fluid chamber into the waste fluid downstream of the main chamber; and a drain conduit configured to deliver a mixture of the waste fluid and the decontamination fluid from the waste chamber to a drain.

2 . The waste decontamination system of claim 1 , further comprising:
a decontamination pump configured to drive the decontamination fluid from the decontamination fluid chamber through the decontamination conduit and into the waste fluid.

3 . The waste decontamination system of claim 2 , further comprising:
a weight sensor configured to detect a weight contained in the main chamber; and a control system configured to:
receive an input of the weight detected by the weight sensor; and
output a signal to control the decontamination pump to drive the decontamination fluid from the decontamination fluid chamber into the waste fluid based on the received input of the detected weight.

4 . The waste decontamination system of claim 2 , wherein the decontamination pump is further configured to drive the waste fluid from the main chamber to the waste chamber.

5 . The waste decontamination system of claim 1 , further comprising:
a plurality of decontamination pumps, wherein a first pump of the decontamination pumps is configured to drive the decontamination fluid from the decontamination fluid chamber through the decontamination conduit and into the waste fluid at a first flow rate, and wherein a second pump of the decontamination pumps is configured to drive the waste fluid from the main chamber to the waste chamber at a second flow rate.

6 . The waste decontamination system of claim 5 , further comprising:
a control system configured to output at least one signal to control the plurality of decontamination pumps, wherein the at least one signal corresponds to at least one of the first flow rate and the second flow rate.

7 . The waste decontamination system of claim 6 , wherein the second flow rate is equal to the first flow rate.

8 . The waste decontamination system of claim 5 , wherein the first flow rate is proportional to a rate of flow into the waste chamber.

9 . The waste decontamination system of claim 1 , further comprising:
a drain pump configured to drive the mixture of the waste fluid and the decontamination fluid from the waste chamber to the drain through the drain conduit.

10 . The waste decontamination system of claim 1 , further comprising:
a valve positioned downstream of an outlet of the waste chamber; and a control system configured to output a signal to actuate the valve to an open position to facilitate flow of the waste fluid and the decontamination fluid out of the waste chamber.

11 . The waste decontamination system of claim 1 , further comprising:
a plurality of secondary containment chambers, wherein the plurality of secondary containment chambers are configured to contain an overflow of waste fluid from each of the main chamber, the waste chamber, and the decontamination fluid chamber; and a plurality of drain conduits, wherein the plurality of drain conduits are configured to deliver the overflow of waste fluid contained in each of the plurality of secondary containment chambers from the plurality of secondary containment chambers to the drain.

12 . The waste decontamination system of claim 1 , wherein the decontamination fluid is introduced to the waste fluid in the waste conduit.

13 . The waste decontamination system of claim 12 , wherein the decontamination fluid is introduced to the waste fluid upstream of the waste chamber.

14 . A waste decontamination system comprising:
a main chamber configured to contain an organ for perfusing; a waste chamber configured to receive waste fluid from the main chamber; a decontamination fluid chamber configured to store decontamination fluid; a decontamination conduit configured to introduce the decontamination fluid from the decontamination fluid chamber into the waste fluid downstream of the main chamber; a drain conduit configured to deliver a mixture of the waste fluid and the decontamination fluid from the waste chamber to a drain; a plurality of decontamination pumps each positioned downstream of each of the main chamber and the decontamination fluid chamber and upstream of the waste chamber; and a controller communicatively coupled to the plurality of decontamination pumps and configured to:
output a first signal to a first pump of the plurality of decontamination pumps, the first signal causing the first pump to provide a first flow rate of at least one of the waste fluid or the decontamination fluid to the waste chamber, and
output a second signal to a second pump of the plurality of decontamination pumps, the second signal causing the second pump to provide a second flow rate of at least one of the waste fluid or the decontamination fluid to the waste chamber.

15 . The waste decontamination system of claim 14 , wherein the first flow rate is proportional to a rate of flow into the waste chamber.

16 . The waste decontamination system of claim 15 , wherein the second flow rate is equal to the first flow rate.

17 . The waste decontamination system of claim 14 , further comprising:
a plurality of secondary containment chambers, wherein the plurality of secondary containment chambers are configured to contain an overflow of waste fluid from the main chamber, the waste chamber, and the decontamination fluid chamber; and a plurality of drain conduits, wherein the plurality of drain conduits are configured to deliver the overflow of waste fluid contained in the plurality of secondary containment chambers from the plurality of secondary containment chambers to the drain.

18 . The waste decontamination system of claim 14 , further comprising a drain pump configured to drive the mixture of the waste fluid and the decontamination fluid from the waste chamber to the drain, the drain pump communicably coupled to the controller;
wherein the controller is configured to output a drain signal to the drain pump to facilitate flow of fluid out of the waste chamber.

19 . The waste decontamination system of claim 14 , further comprising a solenoid valve positioned on a bottom of the waste chamber configured to facilitate flow of the mixture of the waste fluid and the decontamination fluid from the waste chamber to the drain, the solenoid valve communicably coupled to the controller;
wherein the controller is configured to output a drain signal to the solenoid valve to actuate the solenoid valve to an open position.

20 . A waste decontamination system for a bioreactor, comprising:
a main chamber configured to contain an organ for perfusing; a waste chamber configured to receive waste fluid from the main chamber; a waste conduit configured to deliver the waste fluid from the main chamber to the waste chamber; a decontamination fluid chamber configured to store decontamination fluid; a decontamination conduit configured to introduce the decontamination fluid from the decontamination fluid cham

Assignees

• United Therapeutics Corp

Inventors

• Bonvillain Ryan
• Cheadle John
• Petersen Thomas

Classifications

• A61L2/022

  Filtration · CPC title

• G01L7/00

  Measuring the steady or quasi-steady pressure of a fluid or a fluent solid material by mechanical or fluid pressure-sensitive elements ({G01L11/004 takes precedence;} transmitting or indicating the displacement of mechanical pressure-sensitive elements by electric {, e.g., photoelectric} or magnetic means G01L9/00; measuring differences of two or more pressure values G01L13/00; measuring two or more pressure values simultaneously G01L15/00) · CPC title

• G01K13/02

  for measuring temperature of moving fluids or granular materials capable of flow · CPC title

• G01G19/00

  Weighing apparatus or methods adapted for special purposes not provided for in the preceding groups {(electric measuring arrangements involving comparison with a reference value G01R17/00)} · CPC title

• A61L27/36

  containing ingredients of undetermined constitution or reaction products thereof {, e.g. transplant tissue, natural bone, extracellular matrix (isolated natural compounds, e.g. collagen A61L27/24)} · CPC title