Immobilization strategies for enhancing sensitivity of electrochemical aptamer-based sensors

Patent metadata

Field	Value
Publication number	US-12455256-B2
Application number	US-202318492169-A
Country	US
Kind code	B2
Filing date	Oct 23, 2023
Priority date	Dec 13, 2021
Publication date	Oct 28, 2025
Grant date	Oct 28, 2025

Abstract

The subject invention provides methods for fabricating electrochemical aptamer-based (E-AB) sensors with enhanced sensitivity, signal-to-noise ratios, LOD, and improved stability and reproducibility. The subject invention also provides methods for aptamer immobilization on the surface of the electrode, which favors sufficient spacing between aptamers at the microscale to achieve optimal target recognition, folding, and signal transduction. The E-AB sensors of the subject invention provide superior sensing regardless of the sequence or structure of the bound aptamers or the physiochemical properties of the target.

First claim

We claim:

1. An electrochemical aptamer-based (E-AB) sensor comprising an electrode having structure-switching aptamers evenly immobilized on the surface of the electrode without forming clusters, the structure-switching aptamers being in an unfolded single-stranded state, and the structure-switching aptamers being spaced at the microscopic level with a distance between each aptamer that is sufficient for target binding to each aptamer and subsequent change in aptamer conformation from the unfolded single-stranded state to a target-bound folded state, and each structure-switching aptamer comprising a sequence selected from ADE-25-MB (SEQ ID NO: 4) and COC-32-MB (SEQ ID NO: 5).

2. The E-AB sensor according to claim 1 , the electrode being a solid electrode comprising gold, silver, copper, platinum, palladium, an alloy, or a mixture thereof.

3. The E-AB sensor according to claim 1 , the electrode being a paper-based electrode.

4. The E-AB sensor according to claim 1 , the electrode being a gold electrode.

5. The E-AB sensor according to claim 1 , the electrode further being surface-modified with a backfiller selected from 6-mercapto-1-hexanol (MCH), dithiothreitol (DTT), 9-mercapto-1-nonanol, 2-{2-[2-(2-mercaptoethoxy) ethoxy]ethoxy}ethanol, and combinations thereof.

6. The E-AB sensor according to claim 1 , which is fabricated by a method comprising:
incubating the electrode with an immobilizing solution comprising aptamer-target complexes each comprising an aptamer bound to an analyte, the immobilizing solution having a low ionic strength;
contacting the electrode with a solution comprising a backfiller; and
removing the target from each of the aptamer-target complexes.

7. The E-AB sensor according to claim 6 , the ionic strength being from about 1 mM to about 50 mM.

8. A method for detecting a target selected from cocaine and adenosine in a sample, the method comprising contacting the sample with the E-AB sensor according to claim 1 , and detecting the target in the sample by measuring a current generated upon binding of the target to the aptamers.

9. The method according to claim 8 , the sample being a biological sample or an environmental sample.

Assignees

• Xiao Yi
• Liu Yingzhu
• Canoura Juan
• The Florida International Univ Board Of Trustees

Inventors

• Xiao Yi
• Liu Yingzhu
• Canoura Juan

Classifications

• G01N27/3277 Primary

  being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48) · CPC title

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• G01N33/5438

  Electrodes · CPC title

• G01N27/3276 Primary

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title