Bioluminescent single photon bioreactor and performing absolute quantification of light-producing activity by enzymes

Patent metadata

Field	Value
Publication number	US-12454716-B2
Application number	US-202117515832-A
Country	US
Kind code	B2
Filing date	Nov 1, 2021
Priority date	May 10, 2021
Publication date	Oct 28, 2025
Grant date	Oct 28, 2025

Abstract

A bioluminescent single photon bioreactor for performing absolute quantification of light-producing activity by enzymes includes: a bioreactor that produces a bio-electronic signal; an electronic sensor that receives the bio-electronic signal and produces an electrical transduction signal; and an analyzer that receives the electrical transduction signal and absolutely quantifies light-producing activity by enzymes from the electrical transduction signal, such that the absolute quantification is accomplished quantum mechanically by determination of a second order autocorrelation function.

First claim

What is claimed is:

1. A method implemented by a system of one or more processors, the system being included in a bioluminescent single photon bioreactor and for performing absolute quantification of light-producing activity by enzymes by the bioluminescent single photon bioreactor, the method comprising:
producing, by a pixel detector of the bioluminescent single photon bioreactor, a detector signal;
receiving, by an analyzer of the bioluminescent single photon bioreactor, the detector signal;
determining, by the bioluminescent single photon bioreactor, a time of arrival of the detector signal;
determining, by the bioluminescent single photon bioreactor, an autocorrelation among detector signals for threshold times of arrival of photons at pixel detector according to:
the following equation for a single emitter in the field of view of an objective of the bioluminescent single photon bioreactor:

g

(
2
)

(
τ
)

=

1
-

e

-

τ
T

,
or

g (2) (0) for the number of emitters N in the field of view (FOV) of the objective, such that:
if g (2) (0) is 0, then one enzyme was in the field of view; and
if g (2) (0) is not 0, then the number of emitters N is greater than 1, or noise is present in the detector signal.

2. The method of claim 1 , further comprising:
receiving, by a bioreactor of the bioluminescent single photon bioreactor, the enzyme at a selected concentration;
receiving, by the bioreactor, a substrate;
catalysing a bioreaction involving the substrate and the enzyme in the bioreactor;
producing a bioluminescent light by the bioreaction;
collecting, by the confocal optical module, the bioluminescent light from the bioreactor;
communicating the bioluminescent light from the confocal optical module to the pixel detector; and
detecting, by the pixel detector, the bioluminescent light from the confocal optical module prior to producing, by the pixel detector, the detector signal.

3. The method of claim 1 , further comprising measuring the second-order autocorrelation function g (2) by:
identifying timestamps of photon detection times; and
defining a time interval ΔT for accumulating coincidences.

4. The method of claim 2 , wherein the enzyme is immobilized to a portion of the bioreactor not to move freely through the bioreactor.

5. The method of claim 2 , wherein the enzyme flows into the bioreactor from an enzyme supply and flows through the bioreactor in absence of immobilization to the bioreactor.

6. The method of claim 1 , further comprising:
receiving a fluorophore compound comprising the enzyme in the bioreactor;
receiving excitation light by the fluorophore compound in the bioreactor;
producing fluorescent light by the fluorophore compound in response to receipt of the excitation light;
communicating the fluorescent light from the bioreactor to the confocal optical module;
communicating the fluorescent light from the confocal optical module to the pixel detector; and
detecting the fluorescent light from the confocal optical module by the pixel detector, such that the detector signal further comprises components due to the fluorescent light.

7. Non-transitory computer storage media storing instructions for execution by a system of one or more processors, the system being included in a bioluminescent single photon bioreactor, and the instructions causing the one or more processors to perform operations comprising: producing, by a pixel detector of the bioluminescent single photon bioreactor, a detector signal; receiving, by an analyzer of the bioluminescent single photon bioreactor, the detector signal; determining, by the bioluminescent single photon bioreactor, a time of arrival of the detector signal; determining, by the bioluminescent single photon bioreactor, an autocorrelation among detector signals for threshold times of arrival of photons at pixel detector according to: the following equation for a single emitter in the field of view of an objective of the bioluminescent single photon bioreactor:

g

(
2
)

(
τ
)

=

1
-

e

-

τ
T

,
or

g (2) (0) for the number of emitters N in the field of view (FOV) of the objective, such that: if g (2) (0) is 0, then one enzyme was in the field of view; and if g (2) (0) is not 0, then the number of emitters N is greater than 1, or noise is present in the detector signal.

8. The computer storage media of claim 7 , further comprising:
receiving, by a bioreactor of the bioluminescent single photon bioreactor, the enzyme at a selected concentration;
receiving, by the bioreactor, a substrate;
catalysing a bioreaction involving the substrate and the enzyme in the bioreactor;
producing a bioluminescent light by the bioreaction;
collecting, by the confocal optical module, the bioluminescent light from the bioreactor;
communicating the bioluminescent light from the confocal optical module to the pixel detector; and
detecting, by the pixel detector, the bioluminescent light from the confocal optical module prior to producing, by the pixel detector, the detector signal.

9. The computer storage media of claim 8 , further comprising measuring the second-order autocorrelation function g (2) by:
identifying timestamps of photon detection times; and
defining a time interval ΔT for accumulating coincidences.

10. The computer storage media of claim 8 , wherein the enzyme is immobilized to a portion of the bioreactor not to move freely through the bioreactor.

11. The computer storage media of claim 8 , wherein the enzyme flows into the bioreactor from an enzyme supply and flows through the bioreactor in absence of immobilization to the bioreactor.

12. The computer storage media of claim 8 , further comprising:
receiving a fluorophore compound comprising the enzyme in the bioreactor;
receiving excitation light by the fluorophore compound in the bioreactor;
producing fluorescent light by the fluorophore compound in response to receipt of the excitation light;
communicating the fluorescent light from the bioreactor to the confocal optical module;
communicating the fluorescent light from the confocal optical module to the pixel detector; and
detecting the fluorescent li

Assignees

• Government Of The Us Secretary Of Commerce
• Government Of The United States Of America As Represented By Secretary Of Commerce

Inventors

• Polyakov Sergey Vladimirovich
• Jimenez Ralph

Classifications

• B01L3/508

  Rigid containers without fluid transport within · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title

• B01L2300/023

  Sending and receiving of information, e.g. using Bluetooth® · CPC title

• B01L2200/026

  Fluid interfacing between devices or objects, e.g. connectors, inlet details · CPC title

• G01N21/763

  Bioluminescence · CPC title