Cutibacterium acnes recombinant phages encoding a human protein

Patent metadata

Field	Value
Publication number	US-12454696-B2
Application number	US-202318478500-A
Country	US
Kind code	B2
Filing date	Sep 29, 2023
Priority date	Nov 4, 2020
Publication date	Oct 28, 2025
Grant date	Oct 28, 2025

Abstract

The invention relates to C. acnes strains carrying DNA vectors for the production of recombinant C. acnes phages. The invention encompasses a C. acnes producer cell carrying DNA vectors, with a template for recombination with C. acnes phage genome leading to the insertion of a gene of interest, for the production of recombinant phages that can lead to the transgene expression into C. acnes infected by the recombinant phage. The invention encompasses, C. acnes strains containing these vectors, C. acnes recombinant phages and methods of using these recombinant phages.

First claim

We claim:

1. A recombinant C. acnes phage comprising at least one transgene, wherein said transgene encodes a human protein selected from an antibody, a protein that binds non-covalently to a target, a receptor binding protein, a blood factor, an enzyme, a growth factor, a hormone, an interferon, and a cytokine.

2. The recombinant C. acnes phage according to claim 1 , wherein the recombinant C. acnes phage is further engineered so that its host range is different from the host range of the corresponding wild type C. acnes phage.

3. The recombinant C. acnes phage according to claim 1 , comprising an engineered capsid.

4. The recombinant C. acnes phage according to claim 3 , wherein an antigen is displayed at the surface of the engineered capsid.

5. The recombinant C. acnes phage according to claim 1 , wherein said human protein is an antibody.

6. The recombinant C. acnes phage according to claim 1 , wherein said human protein is a blood factor.

7. The recombinant C. acnes phage according to claim 1 , wherein said human protein is an enzyme.

8. The recombinant C. acnes phage according to claim 1 , wherein said human protein is a growth factor.

9. The recombinant C. acnes phage according to claim 1 , wherein said human protein is hormone.

10. The recombinant C. acnes phage according to claim 1 , wherein said human protein is a cytokine.

11. The recombinant C. acnes phage according to claim 1 , wherein said human protein is an interferon.

12. The recombinant C. acnes phage according to claim 1 , wherein said human protein is a protein that binds non-covalently to a target.

13. The recombinant C. acnes phage according to claim 1 , wherein said human protein is a receptor binding protein.

14. The recombinant C. acnes phage according to claim 1 , wherein said human protein is selected from an antibody, a growth factor, a hormone, an interferon, and a cytokine.

15. The recombinant C. acnes phage according to claim 1 , wherein said human protein is selected from a growth factor, a hormone, an interferon, and a cytokine.

Assignees

• Eligo Bioscience

Inventors

• Leveau Aymeric
• Canadas Blasco Inès
• Mathieu Aurélie
• Decrulle Antoine

Classifications

• C12N9/64

  derived from animal tissue · CPC title

• A61K39/02

  Bacterial antigens · CPC title

• C12N2800/80

  Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites · CPC title

• C12N2800/101

  for bacteria · CPC title

• C12N2795/10343

  viral genome or elements thereof as genetic vector · CPC title