US12453360B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12453360-B2 |
| Application number | US-202217723123-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 18, 2022 |
| Priority date | Oct 18, 2019 |
| Publication date | Oct 28, 2025 |
| Grant date | Oct 28, 2025 |
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An acetic acid bacteria-containing composition comprising nonanal and methylheptenone, and having a peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone), as determined by stir bar sorptive extraction thermal desorption gas chromatography-mass spectrometry (SBSE-GC/MS), of 1:10 to 100:1.
Opening claim text (preview).
The invention claimed is: 1. A method for producing an acetic acid bacteria-containing composition, the method comprising (i) culturing an acetic acid bacteria, (ii) collecting the acetic acid bacteria after step (i), and (iii) drying the acetic acid bacteria after step (ii), wherein: the acetic acid bacteria is Acetobacter malorum; step (i) further comprises mixing the acetic acid bacteria with nonanal and methylheptenone so that the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone), as determined by solid-phase microextraction gas chromatography-mass spectrometry, is 1:10 to 100:1; and in step (i), the acetic acid bacteria comprises acetic acid bacteria, dimethyl trisulfide, and 2-tridecanone so that the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone) is 1:10 to 100:1, the peak area ratio of nonanal to dimethyl trisulfide (nonanal: dimethyl trisulfide) is 1:100 to 50:1, and the peak area ratio of nonanal to 2-tridecanone (nonanal:2-tridecanone) is 1:100 to 100:1. 2. The method for producing the acetic acid bacteria-containing composition according to claim 1 , further comprising culturing the acetic acid bacteria in a medium containing vinegar in step (i). 3. The method for producing the acetic acid bacteria-containing composition according to claim 1 , further comprising subjecting the acetic acid bacteria obtained after step (iii) to a high-temperature treatment under low pH conditions. 4. The method for producing the acetic acid bacteria-containing composition according to claim 2 , further comprising subjecting the acetic acid bacteria obtained after step (iii) to a high-temperature treatment under low pH conditions. 5. The method for producing the acetic acid bacteria-containing composition according to claim 1 , further comprising adding water to the acetic acid bacteria obtained after step (ii) for washing. 6. The method for producing the acetic acid bacteria-containing composition according to claim 4 , further comprising adding water to the acetic acid bacteria obtained after step (ii) for washing. 7. The method for producing the acetic acid bacteria-containing composition according claim 1 , wherein the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone) is 1:1 to 50:1. 8. The method for producing the acetic acid bacteria-containing composition according to claim 7 , wherein the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone) is 1:1 to 20:1. 9. The method for producing the acetic acid bacteria-containing composition according to claim 8 , wherein the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone) is 2:1 to 10:1. 10. The method for producing the acetic acid bacteria-containing composition according to claim 1 , wherein the peak area ratio of nonanal to dimethyl trisulfide (nonanal: dimethyl trisulfide) is 1:100 to 15:1. 11. The method for producing the acetic acid bacteria-containing composition according to claim 10 , wherein the peak area ratio of nonanal to dimethyl trisulfide (nonanal: dimethyl trisulfide) is 1:100 to 10:1. 12. The method for producing the acetic acid bacteria-containing composition according to claim 11 , wherein the peak area ratio of nonanal to dimethyl trisulfide (nonanal: dimethyl trisulfide) is 1:30 to 10:1. 13. The method for producing the acetic acid bacteria-containing composition according to claim 1 , wherein the peak area ratio of nonanal to 2-tridecanone (nonanal:2-tridecanone) is 1:50 to 50:1. 14. The method for producing the acetic acid bacteria-containing composition according to claim 13 , wherein the peak area ratio of nonanal to 2-tridecanone (nonanal:2-tridecanone) is 1:10 to 50:1. 15. The method for producing the acetic acid bacteria-containing composition according to claim 14 , wherein the peak area ratio of nonanal to 2-tridecanone (nonanal:2-tridecanone) is 1:6 to 25:1.
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