Electrotransfer and electrophoresis devices, systems, and methods

Patent metadata

Field	Value
Publication number	US-12449396-B2
Application number	US-202118012236-A
Country	US
Kind code	B2
Filing date	Jul 7, 2021
Priority date	Jul 28, 2020
Publication date	Oct 21, 2025
Grant date	Oct 21, 2025

Abstract

The present disclosure provides devices and systems for gel electrophoresis and/or electrotransfer and/or detection of biomolecules. Devices for electrotransfer comprise: one or more receptacles comprising: a biomolecule receiving material (BMR); a first and second electrode; wherein the second electrode is located after and/or distal to an area where biomolecules are received OR wherein both electrodes are arranged to allow current flow in at least two directions, wherein the second direction of current flow is along the plane of the BMR. Devices and systems can be adapted to perform electrophoresis, wherein the receptacle further comprises a matrix for electrophoretic separation of biomolecules. Devices and systems can be further adapted to perform detection, wherein the receptacle further comprises reagent dispensing channels or ports and/or reagents to detect electrotransferred biomolecules. Devices and systems of the disclosure can be microfluidic devices. Methods using the systems and devices of the disclosure are also provided.

First claim

What is claimed is:

1. A device for electrotransfer of biomolecules comprising:
at least one receptacle comprising:
a biomolecule receiving material;
a first electrode; and
a second electrode, wherein the second electrode is in electrical contact with the biomolecule receiving material, and is located distal to and/or posterior to an area where biomolecules are received
wherein the biomolecule receiving material is placed on the receptacle.

2. The device of claim 1 , wherein the biomolecule receiving material is located in a plane and wherein the second electrode is located above the biomolecule receiving material or wherein the second electrode is located along the same plane as the biomolecule receiving material or wherein the second electrode is located below the biomolecule receiving material.

3. The device of claim 1 ,
wherein the first and second electrodes are arranged to allow current flow in at least two directions, wherein a first direction of current flow is transverse to a plane of the biomolecule receiving material or wherein a first direction of current flow is diagonal to a plane of the biomolecule receiving material or wherein a first direction of current flow is perpendicular to a plane of the biomolecule receiving material, and,
wherein a second direction of current flow is along a plane of the biomolecule receiving material.

4. The device of claim 1 , wherein the receptacle is a channel, a microfluidic channel, a surface, a glass slide, a plastic slide, a vessel, a container, or a chamber.

5. The device of claim 1 , comprising a plurality of receptacles.

6. The device of claim 1 , wherein the receptacle is made of a transparent material.

7. The device of claim 1 , wherein the device is a microfluidic device.

8. The device of claim 1 , wherein the biomolecule receiving material comprises a membrane, nitrocellulose, PVDF, cellulose acetate, anodic aluminum oxide, or one or more conductive materials embedded in any of the foregoing.

9. The device of claim 8 , wherein the conductive materials embedded in the biomolecule receiving material comprise tantalum, copper, indium, or tin oxide.

10. The device of claim 1 , wherein the biomolecule receiving material comprises one or more ions.

11. The device of claim 1 , wherein the biomolecule receiving material has a pore size of from about 0.2 μM-10 μM.

12. The device of claim 1 , wherein the biomolecule receiving material has a pore size that is different in different areas in the same layer of the biomolecule receiving material.

13. The device of claim 1 , wherein the biomolecule receiving material has one or more layers.

14. The device of claim 13 , wherein each layer of the biomolecule receiving material has a different pore size.

15. The device of claim 1 , wherein the first electrode is positioned above at least a portion of the biomolecule receiving material.

16. The device of claim 15 , wherein the first electrode is further positioned before an area where biomolecules are received onto the biomolecule receiving material.

17. A device of claim 1 , further comprised in a system, comprising one or more of the following:
an instrument or a dock that can provide a power-supply; a means to activate and/or inactivate electrodes; capillaries; tubings; channel; microchannels; pumps; valves; robotic arms or motors; means to regulate electrode movement/repositioning; means for dispensing and/regulating flow of reagents; software components to regulate release/timed release of reagents and/or to regulate electrode activation/inactivation and/or to regulate electrode movement/repositioning during operation.

18. A method of electrophoresis and electrotransfer of biomolecules comprising:
1) Loading a sample comprising one or more biomolecules into a sample loading area of a device, the device comprising:
at least one receptacle comprising:
a matrix operable to separate the biomolecules along its length, the matrix having the sample loading area at its anterior end;
a biomolecule receiving material, placed below the matrix;
at least a first electrode and a second electrode;
the first electrode located above at least a portion of the matrix and positioned anterior to the sample loading area;
the second electrode in electrical contact with the biomolecule receiving material and located outside an area where biomolecules are received onto the biomolecule receiving material; and
at least one ion reservoir;

2) Performing electrophoresis of the biomolecules in the matrix by activating both the electrodes;
3) inactivating the first and second electrodes to stop the electrophoresis; and
4) performing electrotransfer of the biomolecules from the matrix onto the biomolecule receiving material by:
(i) moving/positioning the first electrode and a first ion reservoir onto at least a portion of the top of the matrix;
(ii) moving/positioning the second electrode and a second ion reservoir past the distal end of the matrix such that the second electrode and the second ion reservoir are electrically connected to the biomolecule receiving material;
(iii) activating the first electrode and the second electrode, enabling transfer of the biomolecule from the matrix into the biomolecule receiving material; and
(iv) inactivating the first and second electrodes to stop the electrotransfer once complete.

19. A device for electrotransfer of biomolecules comprising:
at least one receptacle comprising:
a biomolecule receiving material;
a first electrode; and
a second electrode, wherein the second electrode is in electrical contact with the biomolecule receiving material, and is located distal to and/or posterior to an area where biomolecules are received, wherein the biomolecule receiving material has a pore size that is different in different areas in the same layer of the biomolecule receiving material.

20. The device of claim 19 , wherein the biomolecule receiving material is located in a plane and wherein the second electrode is located above the biomolecule receiving material or wherein the second electrode is located along the same plane as the biomolecule receiving material or wherein the second electrode is located below the biomolecule receiving material.

21. The device of claim 19 , wherein the electrodes are arranged to allow current flow in at least two directions, wherein a first direction of current flow is transverse to a plane of the biomolecule receiving material or wherein a first direction of current flow is diagonal to a plane of the biomolecule receiving material or wherein a first direction of current flow is perpendicular to a plane of the biomolecule receiving material, and, wherein a second direction of current flow is along a plane of the biomolecule receiving material.

22. The device of claim 19 , wherein the receptacle is a channel, a microfluidic channel, a surface, a glass slide, a plastic slide, a vessel, a container, or a chamber.

23. The device of claim 19 , comprising a plurality of receptacles.

24. The device of claim 19 , wherein the receptacle is made of a transparent material.

25. The device of claim 19 , wherein the device is a microfluidic device.

26. The device of claim 19 , wherein the biomolecule receiving material is placed on the receptacle.

27. The device of claim 19 , wherein the biomolecule receiving material comprises a membrane, nitrocellulose, PVDF, cellulose acet

Assignees

• Pierce Biotechnology Inc
• Life Technologies Corp

Inventors

• Vattem Krishna
• Webb Brian

Classifications

• G01N27/44717

  Arrangements for investigating the separated zones, e.g. localising zones · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title