Compositions and methods comprising protease variants
US-2016083710-A1 · Mar 24, 2016 · US
Compositions and methods comprising variant microbial proteases
US12448616B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12448616-B2 |
| Application number | US-202418672557-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 23, 2024 |
| Priority date | Jun 6, 2008 |
| Publication date | Oct 21, 2025 |
| Grant date | Oct 21, 2025 |
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- Title
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Abstract
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The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.
First claim
Opening claim text (preview).
What is claimed is: 1. An isolated subtilisin variant comprising the substitution set N76D-D120H-K213N-M222Q, wherein the isolated subtilisin variant has at least 90% amino acid sequence identity with the amino acid sequence of SEQ ID NO: 1, and wherein said substitutions are at position equivalent to the positions of BPN′ subtilisin set forth in SEQ ID NO: 1. 2. An isolated nucleic acid encoding the isolated subtilisin variant set forth in claim 1 . 3. An expression vector comprising said isolated nucleic acid of claim 2 . 4. A host cell comprising said expression vector of claim 3 . 5. A cleaning composition comprising the isolated subtilisin variant set forth in claim 1 . 6. The cleaning composition of claim 5 , wherein said cleaning composition is a laundry detergent. 7. The laundry detergent of claim 6 , wherein said laundry detergent is a heavy duty liquid laundry detergent. 8. The cleaning composition of claim 5 , wherein said cleaning composition is a dish detergent. 9. The cleaning composition of claim 5 , further comprising one or more additional enzymes or enzyme derivatives selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, keratinases, reductases, oxidases, phenol oxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, β-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, and amylases, or mixtures thereof. 10. The cleaning composition of claim 5 , further comprising at least one stabilizing agent. 11. A cleaning composition comprising at least 0.0001 weight percent of the isolated subtilisin variant of claim 1 , and optionally, at least one suitable adjunct ingredient. 12. A method of cleaning, said method comprising the steps of: a) contacting a surface and/or an article comprising a fabric with the cleaning composition of claim 5 ; and b) optionally washing and/or rinsing said surface or article.
Assignees
Inventors
Classifications
Subtilisin (3.4.21.62) · CPC title
Preparations containing enzymes {, e.g. protease or amylase} · CPC title
Soft surfaces, e.g. textile · CPC title
Enzymes · CPC title
derived from bacteria {or Archaea} · CPC title
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Frequently asked questions
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- What does patent US12448616B2 cover?
- The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.
- Who is the assignee on this patent?
- Danisco Us Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/54. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 21 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).