Synthetic peptide for repressing transcription and/or gene expression from a binding site of interest
US-2016362452-A1 · Dec 15, 2016 · US
Mutant polymerases and methods of using the same
US12448612B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12448612-B2 |
| Application number | US-202117924637-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 11, 2021 |
| Priority date | May 11, 2020 |
| Publication date | Oct 21, 2025 |
| Grant date | Oct 21, 2025 |
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- Title
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Abstract
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This application relates to mutant polymerases. This application discloses mutant phage-type RNA polymerases, such as a mutant T7, SP6, and T3 RNA polymerase, may use 2′-modified nucleoside triphosphates or deoxynucleotide triphosphates as substrates. Methods for producing nucleic acid molecules using these mutant polymerases are also disclosed.
First claim
Opening claim text (preview).
What is claimed is: 1. A mutant polymerase comprising: a catalytic domain having at least 95% sequence identity with SEQ ID NO: 4, and wherein the mutant polymerase comprises one or more mutations at position V459, G231, and/or V365 relative to SEQ ID NO: 4. 2. The mutant polymerase of claim 1 , wherein the mutant polymerase further comprises an N-terminal domain having at least 80% sequence identity with the amino acid sequence corresponding to amino acids 1-324 of SEQ ID NO: 1. 3. The mutant polymerase of claim 1 , wherein the mutant polymerase comprises: (i) a catalytic domain having at least 98% sequence identity with SEQ ID NO: 4, and one or more mutations at position V459, G231, and/or V365 relative to SEQ ID NO: 4; and (ii) an N-terminal domain having at least 80% sequence identity with amino acid sequence corresponding to amino acids 1-324 of SEQ ID NO: 1. 4. The mutant polymerase of claim 3 , wherein the catalytic domain and the N-terminal domain are non-covalently linked. 5. The mutant polymerase of claim 1 , wherein the mutant polymerase comprises a non-conservative substitution at V459. 6. The mutant polymerase of claim 1 wherein the mutant polymerase comprises V459G, V459A, V459T, V459S, V459C, V459P, V459F, V459Y, V459W, V459H, V459K, V459R, V459D, V459E, V459N, or V459Q.
Assignees
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Classifications
DNA-directed RNA polymerase (2.7.7.6) · CPC title
Polynucleotides, e.g. nucleic acids, oligoribonucleotides · CPC title
- C12N9/1247Primary
DNA-directed RNA polymerase (2.7.7.6) · CPC title
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- What does patent US12448612B2 cover?
- This application relates to mutant polymerases. This application discloses mutant phage-type RNA polymerases, such as a mutant T7, SP6, and T3 RNA polymerase, may use 2′-modified nucleoside triphosphates or deoxynucleotide triphosphates as substrates. Methods for producing nucleic acid molecules using these mutant polymerases are also disclosed.
- Who is the assignee on this patent?
- Thermo Fisher Scientific Baltics Uab
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1247. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 21 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).