Method for activating crop seed by high-voltage electric field cold plasma (HVCP), and use thereof

Patent metadata

Field	Value
Publication number	US-12446504-B2
Application number	US-202218548796-A
Country	US
Kind code	B2
Filing date	Dec 5, 2022
Priority date	Sep 1, 2022
Publication date	Oct 21, 2025
Grant date	Oct 21, 2025

Abstract

The present disclosure relates to a method for activating a crop seed by high-voltage electric field cold plasma (HVCP), and use thereof, belonging to the technical field of crop planting. The present disclosure provides a method for activating a crop seed by HVCP, including the following steps: (1) mixing a crop seed with water and immersing the crop seed in water for 4 h to 24 h to obtain an immersed crop seed; and (2) conducting discharge activation on the immersed crop seed at a voltage of 80 kV to 130 kV to obtain an activated crop seed. In the present disclosure, the method can improve heat-resistant and disease-resistant properties of crops, and provide a scientific basis for subsequent acquisition of excellent seeds.

First claim

What is claimed is:

1. A method for mutagenizing a crop seed by high-voltage electric field cold plasma (HVCP), comprising following steps:
(1) mixing a crop seed with water and immersing the crop seed in water for 4 h to 8 h to obtain an immersed crop seed; and
(2) conducting discharge activation by HVCP on the immersed crop seed at a voltage of 130 kV,
wherein the discharge activation is conducted for 2 h to 4 h, and
wherein the crop is Brassica campestris ssp. chinensis Makino.

2. The method according to claim 1 , wherein the mixing of the crop seed with the water is conducted at a mass-to-volume ratio of 1:4 to 1:6.

3. The method according to claim 2 , wherein the mixing of the crop seed with the water is conducted at a mass-to-volume ratio of 1:5.

4. The method according to claim 1 , wherein the immersing is conducted in dark; and
the immersing is conducted at a water temperature of 18° C. to 22° C.

5. The method according to claim 4 , wherein the immersing is conducted at a water temperature of 20° C.

6. The method according to claim 1 , wherein the discharge activation by HVCP is conducted at 18° C. to 25° C.

7. The method according to claim 6 , wherein the discharge activation by HVCP is conducted at 20° C.

8. A method for mutation breeding by HVCP comprising following steps:
(1) mixing a crop seed with water and immersing the crop seed in water for 4 h to 8 h to obtain an immersed crop seed; and
(2) conduction discharge activation by HVCP on the immerse crop seed at a voltage of 130 kV,
wherein the discharge activation is conducted for 2 h to 4 h, and
wherein the crop is Brassica campestris ssp. chinensis Makino.

Assignees

• Nanjing Suman Plasma Eri Co Ltd
• Nj Tianliang Bioengineering Tech Co Ltd
• Univ Nanjing Agricultural

Inventors

• Hou Xilin
• Xiao Dong
• He Ying
• Li Ying
• Zhang Jianhao
• Wan Jinglin

Classifications

• A01H6/20

  Brassicaceae, e.g. canola, broccoli or rucola · CPC title

• A01C1/00

  Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/06 Primary

  Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82}) · CPC title