Means to detect whether acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus is virulent or non-virulent

Patent metadata

Field	Value
Publication number	US-12442817-B2
Application number	US-202017641402-A
Country	US
Kind code	B2
Filing date	Sep 10, 2020
Priority date	Sep 12, 2019
Publication date	Oct 14, 2025
Grant date	Oct 14, 2025

Abstract

A method of monitoring acute hepatopancreatic necrosis disease in shrimp is disclosed. More specifically, detection of the presence of alkaline phosphatase Phox enzyme, or, of the PiRAVP and/or the PirBVP toxins of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus correlates with non-virulence or virulence, respectively, of the bacterium is disclosed. Hence, an assay capable of detecting the Phox enzyme and/or the toxins could be very useful to monitor the disease in shrimp.

First claim

The invention claimed is:

1. A method of phenotyping Vibrio parahaemolyticus as non-virulent, the method comprising:
providing a sample comprising shrimp culture water;
utilizing antibodies or fragments thereof that bind to alkaline phosphatase Phox enzyme of V. parahaemolyticus to phenotype the V. parahaemolyticus as non-virulent; and
culturing the shrimp without disease-mitigation treatment if the V. parahaemolyticus are phenotyped as non-virulent.

2. The method according to claim 1 , whereby acute hepatopancreatic necrosis disease in shrimp is monitored.

3. An assay comprising:
antibodies or fragments thereof that bind to the alkaline phosphatase Phox enzyme of Vibrio parahaemolyticus.

4. A method of using the assay according to claim 3 to phenotype V. parahaemolyticus as non-virulent or as virulent, the method comprising: interacting the assay with a sample.

5. The assay according to claim 4 , wherein the assay is a lateral flow immunochromatographic assay or an enzyme-linked immunosorbent assay.

6. A process of phenotyping Vibrio parahaemolyticus as non-virulent, the process comprising:
providing a sample comprising shrimp culture water;
bringing the sample into contact with antibodies or fragments thereof that bind to alkaline phosphatase Phox enzyme of V. parahaemolyticus;
determining whether the alkaline phosphatase Phox enzyme of V. parahaemolyticus is present in the sample;
classifying the V. parahaemolyticus s non-virulent when the alkaline phosphatase Phox enzyme is present in the sample; and
culturing the shrimp without disease-mitigation treatment upon classification of the V. parahaemolyticus as non-virulent.

7. The method according to claim 4 , wherein the assay is a lateral flow immunochromatographic assay or an enzyme-linked immunosorbent assay.

Assignees

• Univ Gent

Inventors

• Bossier Peter
• Kumar Vikash
• Roy Suvra

Classifications

• G01N2333/916

  acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4) · CPC title

• G01N2333/28

  from Vibrionaceae (F) · CPC title

• G01N33/573 Primary

  for enzymes or isoenzymes · CPC title

• G01N33/56911 Primary

  Bacteria · CPC title