Method and system for imaging a blood sample

Patent metadata

Field	Value
Publication number	US-12442029-B2
Application number	US-202217882702-A
Country	US
Kind code	B2
Filing date	Aug 8, 2022
Priority date	Jul 1, 2013
Publication date	Oct 14, 2025
Grant date	Oct 14, 2025

Abstract

Apparatus and methods are described including introducing a cell suspension comprising red blood cells into a carrier that is a closed cavity that includes a base surface and a closed top, via an inlet defined by the carrier. The cells in the cell suspension are allowed to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier. At least one microscope image of at least a portion of the monolayer of cells is acquired. Other applications are also described.

First claim

The invention claimed is:

1. A method comprising:
using a carrier that is a closed cavity that includes an inlet into the closed cavity, a base surface, and a closed top;
introducing, using capillary forces, a cell suspension comprising red blood cells from outside the carrier into the closed cavity via the inlet;
allowing the cells, including the red blood cells, in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier, such that after the cell suspension settles on the base surface, the density of the cells of the cell suspension is between about 10,000 and about 30,000 cells per mm 2 , and
acquiring at least one microscope image of at least a portion of the monolayer of cells.

2. The method according to claim 1 , wherein allowing the cells in the cell suspension to settle on the base surface of the carrier to form the monolayer of cells on the base surface of the carrier comprises allowing a period of time between 20 seconds and 5 minutes for the cells to settle as a monolayer on the base surface.

3. The method according to claim 1 , wherein a vertical height of the carrier is between 20 micrometers and 300 micrometers.

4. The method according to claim 1 , wherein acquiring the at least one microscope image comprises acquiring a plurality of microscope images, at least two of which are provided under different conditions, the different conditions being selected from the group consisting of: different portions of the base surface being imaged, and different illumination conditions being used.

5. The method according to claim 1 , wherein the cell suspension has a concentration that is such that when substantially all of the cells in the cell suspension are allowed to settle on the base surface of the carrier to form the monolayer of cells, a monolayer is formed on the surface with substantially no overlap between the cells.

6. The method according to claim 1 , wherein selecting the microscope objective lens comprises selecting a lens that permits acquiring an image of at least one object having a height with respect to the base surface of no more than 3 micrometers.

7. The method according to claim 1 , further comprising diluting a blood sample comprising the cells by a dilution factor to obtain the cell suspension.

8. The method according to claim 7 , wherein the dilution factor is between 50 and 300.

9. The method according to claim 1 , further comprising analyzing the at least one image to determine based thereon presence or absence of a pathogen in the cell suspension.

10. The method according to claim 9 , wherein analyzing the at least one image to determine based thereon presence or absence of a pathogen in the cell suspension comprises analyzing the at least one image to determine based thereon presence or absence of a blood infecting protozoa in the cell suspension.

11. The method according to claim 10 , wherein the blood infecting protozoa is selected from the genus consisting of Trypanosoma, Plasmodium; Toxoplasma and Babesia , and wherein analyzing the at least one image to determine based thereon presence or absence of the pathogen in the cell suspension comprises analyzing the at least one image to determine based thereon presence or absence of the blood infecting protozoa from the selected genus in the cell suspension.

12. A method comprising:
using a carrier that is a closed cavity that includes an inlet into the closed cavity, a base surface, and a closed top;
introducing, using capillary forces, a cell suspension comprising red blood cells from outside the carrier into the closed cavity via the inlet;
allowing the cells, including the red blood cells, in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier; and
acquiring at least one microscope image of at least a portion of the monolayer of cells,
wherein a vertical height of the cell suspension when settled on the base surface is between 20 micrometers and 1000 micrometers, and
wherein the at least one microscope image is obtained by a microscope set to a Depth of Field that is between 0.5 micrometers and 10 micrometers.

13. A method comprising:
using a carrier that is a closed cavity that includes an inlet into the closed cavity, a base surface, and a closed top;
introducing, using capillary forces, a cell suspension comprising red blood cells from outside the carrier into the closed cavity via the inlet;
allowing the cells, including the red blood cells, in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier; and
acquiring at least one microscope image of at least a portion of the monolayer of cells,
wherein acquiring the at least one microscope image of the portion of the monolayer of cells comprises acquiring the at least one microscope image using a microscope set to a Depth of Field that is not more than 20 percent of a vertical height of the cell suspension when settled on the base surface, and
wherein the at least one microscope image is obtained using a microscope set to a Depth of Field that is not more than 15 percent of the vertical height of the cell suspension when settled on the base surface.

Assignees

• S D Sight Diagnostics Ltd

Inventors

• Pollak Joseph Joel
• Houri Yafin Arnon
• Salpeter Seth J

Classifications

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title

• G01N15/1433

  using image recognition · CPC title

• G02B21/367

  providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison · CPC title

• G02B21/34

  Microscope slides, e.g. mounting specimens on microscope slides · CPC title