Serum-free cell culture medium
US-12331341-B2 · Jun 17, 2025 · US
Serum-free medium for avian vaccine production and uses thereof
US12441989B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12441989-B2 |
| Application number | US-202017414759-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 16, 2020 |
| Priority date | Jan 17, 2019 |
| Publication date | Oct 14, 2025 |
| Grant date | Oct 14, 2025 |
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Abstract
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A method includes cultivating primary cells in a serum-free medium supplemented with peptides and peptones derived from plant or vegetable sources. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses.
First claim
Opening claim text (preview).
The invention claimed is: 1. A culture medium which comprises: a) basal media free of human or animal protein, and b) plant-derived tryptone, wherein the culture medium is used for viral propagation in animal cells. 2. The culture medium of claim 1 , wherein the basal media is Eagle's Minimal Essential Medium (EMEM). 3. The culture medium of claim 1 , wherein the basal media is Dulbecco's Modified Eagle Medium (DMEM). 4. The culture medium of claim 1 , further comprising an antibiotic. 5. The culture medium of claim 1 , further comprising yeast extract. 6. The culture medium of claim 1 , further comprising a salt. 7. The culture medium of claim 1 , further comprising a carbohydrate. 8. The culture medium of claim 1 , further comprising additional amino acids. 9. The culture medium of claim 1 , further comprising one or more metal ions, vitamins, or cofactors. 10. The culture medium of claim 1 , further comprising at least one buffer selected from CaCl 2 ·2H 2 O, MgSO 4 ·7H 2 O, NaH 2 PO 4 ·2H 2 O, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and 3-(N-morpholino)-propanesulfonic acid (MOPS) in an amount sufficient to maintain the medium within the pH range of 6.5-7.5. 11. A method of viral propagation, comprising, a) culturing avian embryonic cells in the culture medium of claim 1 , b) establishing an adherent or non-adherent cell culture of the avian embryonic cells capable of proliferating in the basal medium free of human or animal protein, c) infecting the avian embryonic cells with a virus selected from poxvirus or poxvirus vector, infectious bursal disease virus, Rispens, SB-1, Turkey Herpesvirus (HVT), or any Marek's serotype 1, 2, or 3 viral vector when the cell density reaches at least 1.0×10 6 cells/mL, d) culturing the infected avian embryonic cells in the culture medium of claim 1 until cytopathic effect (CPE) reaches at least 50%, and e) harvesting the virus.
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Methods of production or purification of viral material · CPC title
Methods of production or purification of viral material · CPC title
Methods of production or purification of viral material · CPC title
Medium free of human- or animal-derived components · CPC title
Metals; Metal chelators · CPC title
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Frequently asked questions
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- What does patent US12441989B2 cover?
- A method includes cultivating primary cells in a serum-free medium supplemented with peptides and peptones derived from plant or vegetable sources. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses.
- Who is the assignee on this patent?
- Boehringer Ingelheim Animal Health Usa Inc, Boehringer Ingelheim Vetmedica Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12N7/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 14 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).