Microscopy unit

Patent metadata

Field	Value
Publication number	US-12436101-B2
Application number	US-202217836561-A
Country	US
Kind code	B2
Filing date	Jun 9, 2022
Priority date	Dec 12, 2019
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

Apparatus and methods are described for use with a microscopy unit that comprises an objective lens and a microscope camera. A cantilever includes an objective lens housing. A motor moves the cantilever along a direction of the optical axis of the objective lens. The cantilever is configured, during the movement of the cantilever, to support the objective lens within the objective lens housing such that an optical axis of the objective lens is aligned with the camera, without the objective lens being directly connected to the camera. Other applications are also described.

First claim

The invention claimed is:

1. An Apparatus for use with a microscopy unit that comprises an objective lens and a microscope camera, and for use with a bodily sample that contains an analyte disposed within water, the apparatus comprising:
a cantilever comprising an objective lens housing;
a motor configured to move the cantilever along a direction of an optical axis of the objective lens;
a sample chamber configured to receive the bodily sample, a height of the sample chamber being unknown;
an optical-density-measurement unit comprising:
one or more light sources configured to:

direct light through the sample at a first wavelength band, at which absorption of light by the analyte is relatively high, and
direct light through the sample at a second wavelength band that is centered around a wavelength of between 1.200 nm and 3,000 nm; and
one or more light detectors configured to detect light that passes through the sample at each of the first and second wavelength bands; and
at least one computer processor configured, based upon the detected light at each of the first and second wavelength bands, to determine a concentration of the analyte within the bodily sample, without directly measuring the height of the sample chamber.

2. The apparatus according to claim 1 , wherein the motor comprises a stepper motor.

3. The apparatus according to claim 1 , wherein the objective lens includes an infinity-corrected objective lens and the objective lens housing is configured to house the infinity-corrected objective lens.

4. The apparatus according to claim 1 , wherein the cantilever is configured to support the objective lens without the objective lens being disposed along a rail with respect to the camera.

5. The apparatus according to claim 1 , wherein the apparatus is configured for use with a sample carrier and the apparatus further comprises:
a stage configured to support the sample carrier within the microscopy unit, the stage being configured to be moveable, during an optical-measurement-unit-calibration phase, such that an orientation of the stage can be adjusted with respect to the optical axis of the objective lens;
a base plate;
three or more stage-leveling screws configured to fix an orientation of the stage with respect to the optical axis of the objective lens, by the stage-leveling screws being coupled to the base plate; and
at least one spring-screw component comprising a spring and a screw, the spring-screw component being configured:
to facilitate controlled movement of the stage with respect to base plate, during the optical-measurement-unit-calibration phase; and
subsequent to the orientation of the stage having been calibrated with respect to the optical axis of the objective lens, to fix the stage in position with respect to the base plate, by the screw of the spring-screw component being fastened with respect to the base plate.

6. The apparatus according to claim 1 , wherein the cantilever comprises upper and lower horizontal sheets of a material that are coupled to each other via a vertical coupling element.

7. The apparatus according to claim 6 , further comprising one or more springs disposed between portions of the cantilever and configured to stabilize the portions of the cantilever with respect to each other during motion of the cantilever.

8. The apparatus according to claim 1 , wherein the one or more light sources are further configured to direct light through the sample at a third wavelength band at which absorption of light by both the analyte and water is relatively low, wherein the one or more light detectors are further configured to detect light that passes through the sample at the third wavelength band, and wherein, based upon the detected light that passes through the sample at the third wavelength band, the computer processor is configured to account for scatter by the sample.

9. The apparatus according to claim 1 , wherein the computer processor is configured:
based upon the detected light at the second wavelength band, to determine the height of the sample chamber, and
using the determined height of the sample chamber, to determine additional parameters of the bodily sample.

10. The apparatus according to claim 1 , wherein the one or more light detectors comprise one or more light detectors that are configured to detect light that passes through the sample at the second wavelength band selected from the group consisting of: a germanium-based detector, an In—Ga—Ar-based detector, and a combination thereof.

11. The apparatus according to claim 1 , wherein the one or more light sources are configured to direct light through the sample at a second wavelength band that is centered around a wavelength of between 1,200 nm and 1,600 nm.

12. The apparatus according to claim 11 , wherein the one or more light sources are configured to direct light through the sample at a second wavelength band that is centered around a wavelength of between 1,440 nm and 1,460 nm.

13. The apparatus according to claim 1 , wherein the apparatus is for use with a blood sample.

14. The apparatus according to claim 13 , wherein the computer processor is configured to determine a concentration of a combination of a plurality of variants of hemoglobin within the blood sample.

15. The apparatus according to claim 13 , wherein the one or more light sources are configured to direct light through the sample at a first wavelength band that is centered around an isosbestic point of the plurality of variants of hemoglobin.

16. The apparatus according to claim 1 , wherein the cantilever is configured, during the movement of the cantilever, to support the objective lens within the objective lens housing such that an optical axis of the objective lens is aligned with the camera, without the objective lens being directly connected to the microscope camera.

17. An apparatus for use with a microscopy unit that comprises an objective lens and a microscope camera and for use with a bodily sample that contains n analytes disposed within a medium, the apparatus comprising:
a cantilever comprising an objective lens housing;
a motor configured to move the cantilever along a direction of an optical axis of the objective lens;
a sample chamber configured to receive the bodily sample, a height of the sample chamber being unknown;
an optical-density-measurement unit comprising:
one or more light sources configured to:
direct light through the sample at n wavelength bands, each of the n wavelength bands corresponding to a wavelength band at which absorption of light, by a respective one of the n analytes is relatively high, and
direct light through the sample at a further wavelength band at which absorption of light by the medium is relatively high; and

one or more light detectors configured to detect light that passes through the sample at each of the wavelength bands; and

at least one computer processor configured, based upon the detected light at each of the wavelength bands, to determine a concentration of each of the n analytes within the bodily sample, without directly measuring the height of the sample chamber.

18. The apparatus according to claim 17 , wherein the one or more light sources are configured to direct light through the sample at a still-further wavelength band at which absorption of light by both the medium and the n analytes is relatively low, wherein the one or more light detectors are further configured to detect light that passes through the sample at the still-further wavelength band, and wherein, based upon the detected light tha

Assignees

• S D Sight Diagnostics Ltd

Inventors

• Franklin Amitai
• Ruggiero Trevor
• Levy Sarah
• Pollak Joseph Joel
• Carroll Aidan John
• Pecker Sharon
• Eshel Yochay Shlomo

Classifications

• G02B21/362

  Mechanical details, e.g. mountings for the camera or image sensor, housings (G02B21/364 takes precedence) · CPC title

• G02B21/361

  Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups) · CPC title

• G02B21/02

  Objectives · CPC title

• G01N2021/3148

  using three or more wavelengths · CPC title

• G01N21/314 Primary

  with comparison of measurements at specific and non-specific wavelengths (dual wavelength spectrometry G01J3/427) · CPC title