Apparatus, system, and method using immiscible-fluid-discrete-volumes
US-11162137-B2 · Nov 2, 2021 · US
US12435367B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12435367-B2 |
| Application number | US-202117505085-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 19, 2021 |
| Priority date | Aug 22, 2005 |
| Publication date | Oct 7, 2025 |
| Grant date | Oct 7, 2025 |
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Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
Opening claim text (preview).
What is claimed is: 1. A method comprising: contacting a stream of aqueous sample fluid flowing in a first conduit with a stream of non-aqueous spacing fluid that is immiscible with the aqueous sample fluid to form discrete volumes of the aqueous sample fluid separated from one another by the non-aqueous spacing fluid, wherein the aqueous sample fluid comprises target nucleic acid, and wherein a first plurality of the discrete volumes contains at least one molecule comprising the…
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