Capillary barriers for staged loading of microfluidic devices

Patent metadata

Field	Value
Publication number	US-12435329-B2
Application number	US-202318388047-A
Country	US
Kind code	B2
Filing date	Nov 8, 2023
Priority date	Mar 14, 2013
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

First claim

What is claimed is:

1. An isotachophoresis (ITP) apparatus, the apparatus comprising:
(a) a first microfluidic channel having a terminus at a fluid-interface region;
(b) a second microfluidic channel having a terminus at the fluid-interface region; and
(c) a capillary barrier at the fluid-interface region, wherein the capillary barrier comprises a ramp.

2. The apparatus of claim 1 , further comprising an escape path fluidically coupled to the fluid-interface region.

3. The apparatus of claim 1 , further comprising a vacuum port positioned to release gas pressure from the fluid-interface region.

4. The apparatus of claim 1 , wherein the capillary barrier is at least in part defined by a change in a cross-sectional area, wherein the change in the cross-sectional area is sufficient to arrest flow of a first liquid at the fluid-interface region.

5. The apparatus of claim 1 , further comprising a first open reservoir connected to the first microfluidic channel.

6. The apparatus of claim 5 , further comprising a second open reservoir connected to the second microfluidic channel.

7. A method comprising:
(a) providing an apparatus comprising: (i) a first microfluidic channel comprising a first liquid and having a terminus at a fluid-interface region; (ii) a second microfluidic channel comprising a second liquid and having a terminus at the fluid-interface region; and (iii) a capillary barrier at the fluid-interface region, wherein the capillary barrier comprises a ramp; and
(b) causing the first liquid to flow in the first microfluidic channel until the capillary barrier arrests flow of the first liquid in the first microfluidic channel.

8. The method of claim 7 , further comprising causing the second liquid to flow in the second microfluidic channel until the capillary barrier arrests flow of the second liquid in the second microfluidic channel.

9. The method of claim 7 , further comprising releasing gas pressure via a gas-outflow port, while flow of the first liquid is arrested.

10. The method of claim 7 , wherein the first liquid comprises a leading electrolyte buffer.

11. The method of claim 7 , wherein the second liquid comprises a sample.

12. The method of claim 11 , wherein the sample comprises a nucleic acid.

13. The method of claim 12 , wherein the nucleic acid comprises DNA.

14. The method of claim 12 , wherein the nucleic acid comprises RNA.

15. The method of claim 7 , further comprising causing the first liquid and the second liquid to contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

16. The method of claim 7 , wherein the apparatus further comprises a third microfluidic channel comprising trailing electrolyte (TE) buffer.

17. The method of claim 7 , further comprising applying an electrical field to the first and second liquids.

18. The method of claim 17 , wherein the method is an isotachophoresis (ITP) method.

19. The apparatus of claim 1 , wherein during use, the first and second microfluidic channels and the capillary barrier are configured to cause a first liquid to flow in the first microfluidic channel until the capillary barrier arrests flow of the first liquid in the first microfluidic channel.

20. The apparatus of claim 1 , wherein the capillary barrier further comprises an expansion.

Assignees

• Univ Leland Stanford Junior

Inventors

• Santiago Juan G
• Marshall Lewis A
• Rogacs Anita

Classifications

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• B01L2300/12

  Specific details about materials · CPC title

• B01L2300/0838

  Capillaries · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title