Continuous flow reactor for low pH viral inactivation

Patent metadata

Field	Value
Publication number	US-12435303-B2
Application number	US-201917283281-A
Country	US
Kind code	B2
Filing date	Oct 7, 2019
Priority date	Oct 8, 2018
Publication date	Oct 7, 2025
Grant date	Oct 7, 2025

Abstract

A viral inactivation device including at least one continuous viral inactivation reactor having an inlet, an outlet, and a tubular flow path. The tubular flow path includes a set of alternating turns that form a serpentine pattern between the inlet and the outlet.

First claim

We claim:

1. A viral inactivation device comprising:
at least one continuous viral inactivation reactor including an inlet, an outlet, and a tubular flow path comprising a set of alternating turns that form a serpentine pattern between the inlet and the outlet;
wherein the tubular flow path includes a diameter of from 0.6 cm to 0.7 cm;
and
wherein the set of alternating turns are segmented vertically into at least two stacked layers, wherein each of the at least two stacked layers include a thickness of from 0.7 cm to 1.2 cm.

2. The device of claim 1 , wherein the at least one continuous viral inactivation reactor is an in-line tubular continuous viral inactivation reactor.

3. The device of claim 1 , wherein the set of alternating turns comprises at least two alternating turns having an angle of from 270° to 280°.

4. The device of claim 1 , wherein the set of alternating turns comprises from 2 to 325 or more alternating turns having an angle of from 270° to 280°.

5. The device of claim 1 , wherein each of the at least two stacked layers comprises 12.5 alternating turns in a single plane, wherein each of the alternating turns includes an angle of from 270° to 280°.

6. The device of claim 1 , wherein each of the at least two stacked layers is connected to one another via a 180° turn of the tubular flow path.

7. The device of claim 6 , wherein the at least two stacked layers are 26 layers connected to one another via 25 180° turns of the tubular flow path.

8. The device of claim 1 , wherein the at least one continuous viral inactivation reactor comprises from 2 to 6 viral inactivation reactors connected in series.

9. The device of claim 1 , wherein the set of alternating turns is arranged to generate a vortex to induce mixing a product stream having a laminar flow with a Reynolds Number of from 187.7 to 375.5.

10. The device of claim 1 , wherein the tubular flow path includes dimensions such that a product stream is in the continuous viral inactivation reactor for at least 30 minutes.

Assignees

• Boehringer Ingelheim Int

Inventors

• Parker Stephanie
• Bardliving Cameron Lee
• Coffman jonathan
• Godfrey Scott
• Orozco Raquel

Classifications

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• C12M47/12 Primary

  Purification (C12M47/04 takes precedence) · CPC title

• C12M27/00

  Means for mixing, agitating or circulating fluids in the vessel (by introduction of gas C12M29/06, C12M29/14, mixing in general or mixers per se B01F; mixing in apparatus for chemical or physical processes B01J) · CPC title

• C12M47/00

  Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass (filters in general B01D24/00 - B01D41/00) · CPC title

• C12M23/06 Primary

  Tubular (C12M23/08, C12M23/16 take precedence) · CPC title