Application of compound amino acids in preparation of medicament for improving sensitivity of bacteria to antibiotics

Patent metadata

Field	Value
Publication number	US-12427129-B2
Application number	US-202117787592-A
Country	US
Kind code	B2
Filing date	Jul 20, 2021
Priority date	Aug 6, 2020
Publication date	Sep 30, 2025
Grant date	Sep 30, 2025

Abstract

The invention belongs to the technical field of biomedicament, and more specifically relates to an application of compound amino acids in preparation of a medicament for improving sensitivity of bacteria to antibiotics. Experiments have shown that a combination of compound amino acids and antibiotics can improve the sensitivity of bacteria to antibiotics, promote an entry of antibiotics into bacterial cells, significantly increase an intracellular antibiotic content, and improve a bactericidal effect of antibiotics, thereby reducing a survival rate of bacteria, and treating diseases caused by bacterial infection.

First claim

What is claimed is:

1. A method of preparing a medicament for improving sensitivity of bacteria to an antibiotic(s), comprising combining i) a compound of amino acids with ii) an antibiotic,
wherein in the compound of amino acids, a molar concentration ratio of each amino acid to total amino acids is aspartic acid 2.65%-2.8%, glutamic acid 4.09%-4.23%, serine 2.37%-4.64%, histidine 4.62%-4.81%, glycine 11.25%-11.6%, threonine 5.06%-5.22%, alanine 19.57%-20.11%, arginine 6.88%-7%, tyrosine 0.16%-0.27%, cystine 0.09%-0.21%, valine 6.67%-6.8%, methionine 4.04%-4.17%, tryptophan 0.98%-1.04%, phenylalanine 5.12%-5.27%, isoleucine 4.59%-4.74%, leucine 6.44%-6.45%, lysine acetate 6.59%-6.64%, and proline 6.23%-6.32%;
wherein a total amino acid content of the compound of amino acids within the medicament is 5%-12%;
wherein the antibiotic is selected from a β-lactam antibiotic, a quinolone antibiotic, an aminoglycoside antibiotic, or a combination thereof; and
wherein the bacteria is selected from Escherichia coli, Vibrio alginolyticus, Vibrio parahaemolyticus, Pseudomonas aeruginosa , and Bacillus subtilis.

2. A medicament for improving sensitivity of bacteria to an antibiotic(s), wherein the medicament contains an effective amount of i) a compound of amino acids and ii) an antibiotic,
wherein in the compound of amino acids, a molar concentration ratio of each amino acid to total amino acids is aspartic acid 2.65%-2.8%, glutamic acid 4.09%-4.23%, serine 2.37%-4.64%, histidine 4.62%-4.81%, glycine 11.25%-11.6%, threonine 5.06%-5.22%, alanine 19.57%-20.11%, arginine 6.88%-7%, tyrosine 0.16%-0.27%, cystine 0.09%-0.21%, valine 6.67%-6.8%, methionine 4.04%-4.17%, tryptophan 0.98%-1.04%, phenylalanine 5.12%-5.27%, isoleucine 4.59%-4.74%, leucine 6.44%-6.45%, lysine acetate 6.59%-6.64%, and proline 6.23%-6.32%;
wherein a total amino acid content of the compound of amino acids within the medicament is 5%-12%;
wherein the antibiotic is selected from a β-lactam antibiotic, a quinolone antibiotic, an aminoglycoside antibiotic, or a combination thereof; and
wherein the bacteria is selected from Escherichia coli, Vibrio alginolyticus, Vibrio parahaemolyticus, Pseudomonas aeruginosa , and Bacillus subtilis.

3. The medicament according to claim 2 , wherein a mass ratio of the antibiotic to total amino acids in the compound amino acids is 1:12.5 to 1:1824.

4. A method of improving sensitivity of a bacteria to an antibiotic(s), comprising administering to the bacteria the medicament of claim 2 , and wherein the bacteria is selected from Escherichia coli, Vibrio alginolyticus, Vibrio parahaemolyticus, Pseudomonas aeruginosa , and Bacillus subtilis.

Assignees

• Univ Sun Yat Sen

Inventors

• Li Hui
• Peng Bo
• Peng Xuanxian
• Lai Shishi

Classifications

• A61K31/7036

  having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins · CPC title

• A61K31/546

  containing further heterocyclic rings, e.g. cephalothin · CPC title

• A61K31/4709

  Non-condensed quinolines and containing further heterocyclic rings · CPC title

• A61K31/43

  Compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula [IMAGE cpc-sch-A61K-0952.gif], e.g. penicillins, penems · CPC title

• A61K31/4172

  Imidazole-alkanecarboxylic acids, e.g. histidine · CPC title