Method for controlling microscopic imaging and corresponding microscope control arrangement and microscope

Patent metadata

Field	Value
Publication number	US-12422659-B2
Application number	US-202218074553-A
Country	US
Kind code	B2
Filing date	Dec 5, 2022
Priority date	Dec 10, 2021
Publication date	Sep 23, 2025
Grant date	Sep 23, 2025

Abstract

A method for controlling microscopic imaging of a microscope includes providing a microscope control arrangement configured for receiving a focusing request and for receiving sample information on a sample to be imaged, wherein the microscope control arrangement activates, upon receipt of a focusing request and after having received the sample information, a predefined focusing setting depending on the sample information received for controlling focusing of the microscope for microscopic imaging of the sample.

First claim

The invention claimed is:

1. A method for controlling microscopic imaging of a microscope, the method comprising:
providing a microscope control arrangement configured for receiving a focusing request and for receiving sample information on a sample to be imaged, wherein the sample information comprises a respective sample specification selected from a plurality of predefined sample specifications, wherein the plurality of predefined sample specifications comprises:
the sample being adherent to a sample carrier for carrying the sample;
the sample being detached from the sample carrier for carrying the sample;
the sample having a varying extension in a focal direction;
the sample being able to move; and/or
the sample having a surface topology varying over time;

upon receipt of the focusing request and receiving the sample information, activating, via the microscope control arrangement, a predefined focusing setting depending on the sample information for controlling focusing of the microscope for microscopic imaging of the sample, wherein the activating the predefined focusing setting automatically initiates a focus strategy associated with the respective sample specification, wherein the initiating the focus strategy comprises:
when the respective sample specification specifies the sample as being adherent to a sample carrier for carrying the sample, using a triangulating autofocusing method for determining and/or maintaining a focus point, the focus point being used for sample imaging,
when the respective sample specification specifies the sample as being detached from the sample carrier for carrying the sample or having a varying extension in a focal direction, defining a focus map of one or more focus points, or
when the respective sample specification specifies the sample as being able to move, using an image based autofocusing method by evaluating a stack of images of the sample, each image being captured at a different focal position.

2. The method according to claim 1 , wherein the sample information relates to one or more sample properties in a three dimensional sample space and/or to a kind of the sample carrier carrying the sample and/or to a material of the sample carrier carrying the sample and/or to a sample staining.

3. The method according to claim 1 , wherein no further information by user input is required to be received by the microscope control arrangement in order to activate the predefined focusing setting.

4. The method according to claim 1 , wherein the predefined focusing setting includes an automatic determination of one or more focus points at defined x-y-positions of the sample.

5. The method according to claim 4 , wherein one or more of the one or more focus points are rewritable by a user.

6. The method according to claim 4 , wherein the one or more focus points are determined by a respective autofocusing method depending on the activated predefined focus setting.

7. The method according to claim 1 , wherein the image based autofocusing method includes generating a first stack of images of the sample, each image being captured at a different focal position, using a first focal step size over a first range of focal positions, and generating a second stack of images of the sample using a second focal step size over a second range of focal positions, the second focal step size being smaller than the first focal step size.

8. The method according to claim 1 , wherein the predefined focusing setting is corrected for a drift of focus over time.

9. The method according to claim 8 , wherein the drift of focus is corrected at least in one of the following cases:
after a predetermined number of image acquisition positions at different x-y-positions of the sample;
after a predetermined number of time points of image acquisition;
after a predetermined time interval; or
in case of more than one focus point of one or more focus points at defined x-y-positions of the sample that are included as an automatic determination of the predefined focusing setting, for a predefined number of focus points.

10. The method according to claim 1 , wherein the focus map is defined with a predefined or selectable number and/or density of focus points.

11. The method according to claim 1 , wherein the focus map is determined by an image based autofocusing method evaluating a stack of images of the sample, each image being captured at a different focal position.

12. The method according to claim 11 , wherein, after determining the focus map and during imaging of the sample, an autofocusing control based on a triangulating autofocus method is used for maintaining the focus.

13. The method according to claim 1 , wherein the microscope control arrangement is configured to allow for activating of different microscopic imaging modes, which are selectable independently from the activated predefined focusing setting.

14. A microscope control arrangement comprising one or more processors and adapted for controlling microscopic imaging of a microscope,
wherein the microscope control arrangement is configured for receiving sample information concerning a sample to be imaged and for receiving a focusing request, wherein the sample information comprises a respective sample specification selected from a plurality of predefined sample specifications, wherein the plurality of predefined sample specifications comprises:
the sample being adherent to a sample carrier for carrying the sample;
the sample being detached from the sample carrier for carrying the sample;
the sample having a varying extension in a focal direction;
the sample being able to move; and/or
the sample having a surface topology varying over time; and

wherein the microscope control arrangement is further configured to activate, upon receipt of the focusing request and the sample information, a predefined focusing setting depending on the sample information received for controlling focusing of the microscope for microscopic imaging of the sample, wherein the activating the predefined focusing setting automatically initiates a focus strategy associated with the respective sample specification, wherein the initiating the focus strategy comprises:
when the respective sample specification specifies the sample as being adherent to a sample carrier for carrying the sample, using a triangulating autofocusing method for determining and/or maintaining a focus point, the focus point being used for sample imaging,
when the respective sample specification specifies the sample as being detached from the sample carrier for carrying the sample or having a varying extension in a focal direction, defining a focus map of one or more focus points, or
when the respective sample specification specifies the sample as being able to move, using an image based autofocusing method by evaluating a stack of images of the sample, each image being captured at a different focal position.

15. A microscope control arrangement comprising:
one or more memories for storing computer-executable instructions; and
one or more processors configured to execute the instructions so as to facilitate a method for controlling microscopic imaging of a microscope, the method comprising:
receiving sample information concerning a sample to be imaged and receiving a focusing request, wherein the sample information comprises a respective sample specification selected from a plurality of predefined sample specifications, wherein the plurality of predefined sample specifications comprises:
the sample being adherent to a sample carrier for carrying the sample;
the sample being detached from the s

Assignees

• Leica Microsystems

Inventors

• Schechter Markus
• Hessler Tobias

Classifications

• G02B7/36

  using image sharpness techniques {, e.g. image processing techniques for generating autofocus signals (image data processing per se G06T; in cameras having a solid state image sensor H04N23/67)} · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• H04N23/50

  Constructional details · CPC title

• H04N23/67

  Focus control based on electronic image sensor signals · CPC title

• H04N23/667

  Camera operation mode switching, e.g. between still and video, sport and normal or high- and low-resolution modes · CPC title