Liquid sample loading

Patent metadata

Field	Value
Publication number	US-12420284-B2
Application number	US-202418649768-A
Country	US
Kind code	B2
Filing date	Apr 29, 2024
Priority date	Sep 28, 2017
Publication date	Sep 23, 2025
Grant date	Sep 23, 2025

Abstract

The assembly includes a docking console and a manifold. The docking console includes a cartridge support surface having a first end and a second end. The manifold has one or more wells defined therein. The docking console further includes a manifold retention bracket to releasably hold the manifold against a fluid cartridge supported on the cartridge support surface at an interface position such that the one or more wells are in fluid communication with the fluid cartridge and a biased seal bar to press the fluid cartridge against the manifold held by the manifold retention bracket. A hydrophilic porous frit disposed within at least one of the wells and is to permit liquid to flow through the outlet aperture but prevent gas from passing through the outlet aperture.

First claim

The invention claimed is:

1. A method for dispensing fluid into a fluid cartridge comprising:
placing the fluid cartridge on a cartridge support surface of a docking console such that a positioning device of the docking console engages the fluid cartridge and biases the fluid cartridge or a component of the fluid cartridge into a predetermined position with respect to the cartridge support surface;
placing a manifold having one or more wells on a manifold retention bracket of the docking console such that the manifold is positioned above the fluid cartridge;
moving the manifold retention bracket from a release position to a locking position with respect to the manifold to hold the manifold against the fluid cartridge such that the one or more wells are fluidically connected to the fluid cartridge as a result of the fluid cartridge or the component of the fluid cartridge being in the predetermined position with respect to the cartridge support surface;
dispensing fluid into the one or more wells of the manifold such that the fluid is dispersed into the fluid cartridge;
moving the manifold retention bracket from the locking position to the release position with respect to the manifold; and
removing the manifold and the fluid cartridge from the docking console.

2. The method of claim 1 , wherein the positioning device comprises one or more resilient prongs projecting from the cartridge support surface, and each resilient prong of the one or more resilient prongs extends through a slot formed in the fluid cartridge.

3. The method of claim 1 , wherein moving the manifold retention bracket from the release position to the locking position provides for the one or more wells being fluidically connect to the fluid cartridge, wherein each of the one or more wells is operatively interfaced with an inlet port of the fluid cartridge.

4. The method of claim 1 , wherein moving the manifold retention bracket from the release position to the locking position with respect to the manifold comprises pivoting a clamp arm of the manifold retention bracket with respect to side walls disposed on opposite sides of the cartridge support surface, and wherein the clamp arm is pivotably attached to the side walls.

5. The method of claim 4 , wherein the manifold comprises a first arm having a first shape and projecting from a first end of the manifold and a second arm having a second shape different from the first shape and projecting from a second end of the manifold, and wherein each of the side walls comprises a recess to receive one of the first arm or the second arm such that the manifold is held by the sidewalls in a single orientation.

6. The method of claim 4 , further comprising locking the manifold retention bracket in the locking position by magnetically coupling the clamp arm to at least one of the sidewalls.

7. The method of claim 1 , further comprising pressing the fluid cartridge against the manifold when the manifold retention bracket is in the locking position with a biased seal bar disposed below the fluid cartridge within a recess in the cartridge support surface.

8. The method of claim 7 , wherein the docking console comprises one or more compression springs disposed in the recess between a bottom of the recess and the seal bar and biased against a bottom surface of the seal bar.

9. The method of claim 1 , further comprising after dispensing fluid into the one or more wells, permitting fluid flow from each well of the one or more wells of the manifold into the fluid cartridge and blocking gas flow from the one or more wells to the fluid cartridge, wherein the fluid is a liquid.

10. The method of claim 9 , wherein permitting fluid flow from each well of the one or more wells of the manifold into the fluid cartridge and blocking gas flow from the one or more wells to the fluid cartridge is performed by a hydrophilic porous frit positioned within each well of the one or more wells.

11. The method of claim 1 , wherein moving the manifold retention bracket from the release position to the locking position forms a fluid sealed connection between an outlet aperture of each well of the one or more wells and an inlet port of one or more inlet ports of the fluid cartridge.

12. The method of claim 4 , wherein moving the manifold retention bracket from the release position to the locking position provides contact pressure against a top surface of the manifold with one or more contact elements projecting from the clamp arm.

13. The method of claim 1 , wherein the docking console comprises a rim wall projecting from the cartridge support surface, wherein the rim wall defines a cavity along the cartridge support surface such that the cavity conforms to a shape of the fluid cartridge.

14. The method of claim 13 , wherein the rim wall comprises one or more tabs configured to engage the fluid cartridge disposed within the cavity.

Assignees

• Illumina Inc
• Illumina Cambridge Ltd

Inventors

• Drews Bradley Kent
• Stengel Gudrun
• Blake James Christopher
• Ahamed Mohammed Kafeel
• Becker Michael Steven
• Dangelo Michael
• Nibbe Mark J
• Fuller Daniel L
• Miller Oliver Jon

Classifications

• G01N1/14 Primary

  Suction devices, e.g. pumps; Ejector devices · CPC title

• B01L2300/0681

  Filter · CPC title

• B01L2300/048

  enabling gas exchange, e.g. vents · CPC title

• B01L2300/047

  Additional chamber, reservoir · CPC title

• B01L9/527

  for microfluidic devices, e.g. used for lab-on-a-chip · CPC title