Method for preparing feed by bacteria-enzyme synergistic fermentation

Patent metadata

Field	Value
Publication number	US-12419327-B2
Application number	US-202217680480-A
Country	US
Kind code	B2
Filing date	Feb 25, 2022
Priority date	Sep 30, 2019
Publication date	Sep 23, 2025
Grant date	Sep 23, 2025

Abstract

The disclosure discloses a method for preparing feed by bacteria-enzyme synergistic fermentation, belonging to the technical field of fermentation engineering. According to the disclosure, Lactobacillus plantarum JUN-DY-6, protease and cellulase are used as a starter, and camellia seed meal or rapeseed meal is used as a substrate. The bacteria-enzyme fermentation product has higher yield of organic acids and flavor substances and better palatability, and can be used for preparing feed additives. The L. plantarum of the disclosure can inhibit growth of harmful bacteria such as Escherichia coli, S. aureus and Salmonella in the intestinal tract of poultry and livestock, and is good for health of the intestinal tract. The method increases the added value of camellia seed meal and rapeseed meal, and is conductive to reuse of waste.

First claim

What is claimed is:

1. A method for preparing a feed composition, comprising:
providing a raw material comprising: (a) camellia seed meal, and (b) enzymes, wherein the enzymes comprise alkaline protease and cellulase, to produce a mixture,
adding to the mixture an inoculum of Lactobacillus plantarum ( L. plantarum ) JUN-DY-6 collection number CCTCC M 2017138, at 1 to 5% to produce a fermentation mixture; and
fermenting the fermentation mixture to produce a feed composition,
wherein a moisture content of the raw material is 30 to 50%;
wherein the cellulase is present in the mixture at 300 to 400 U/g substrate;
wherein the alkaline protease is present in the mixture at 800 to 1200 U/g substrate; and
wherein the L. plantarum JUN-DY-6 is present at a concentration of greater than or equal to 10 6 CFU/g substrate.

2. The method according to claim 1 , wherein the fermenting is conducted at 35° C. to 37° C. for at least 20 hours.

3. The method according to claim 1 , wherein the fermenting is conducted for 40 to 60 hours.

Assignees

• Univ Jiangnan

Inventors

• Deng Yu
• Mao Yin
• Li Guohui

Classifications

• A23V2400/169

  Plantarum · CPC title

• C12P1/04

  by using bacteria · CPC title

• C12N9/50 Primary

  Proteinases {, e.g. Endopeptidases (3.4.21-3.4.25)} · CPC title

• C12N9/2437

  Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title

• C12N1/205

  Bacterial isolates · CPC title