Methods and devices for manipulating biological samples

Patent metadata

Field	Value
Publication number	US-12416552-B2
Application number	US-202418921957-A
Country	US
Kind code	B2
Filing date	Oct 21, 2024
Priority date	Feb 1, 2023
Publication date	Sep 16, 2025
Grant date	Sep 16, 2025

Abstract

Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.

First claim

What is claimed is:

1. A system comprising:
a glass solid support;
a receiving array removably attached to the glass solid support, comprising a plurality of receptacles each forming an internal cavity, wherein one or more receptacles comprise a cell or tissue sample; and
a piston array comprising a base and a plurality of spring actuated pistons extending from the base and wherein the pistons are sized and shaped to fit within the internal cavity of the receptacles.

2. The system of claim 1 , wherein the glass solid support further comprises a resist.

3. The system of claim 1 , wherein the glass solid support further comprises polylysine, poly(2-dimethylaminoethyl methacrylate) (PDMAEMA), chitosan, poly(amidoamine) (PAMAM), polyvinylamine (PVAm), or poly(allylamine hydrochloride) (PAH).

4. The system of claim 1 , wherein the tissue sample comprises breast tissue, lung tissue, colon tissue, lymph tissue, kidney tissue, bone tissue, tonsil tissue, or brain tissue.

5. The system of claim 4 , wherein a thickness of the tissue is about 1 μm to about 20 μm.

6. The system of claim 4 , wherein a thickness of the tissue is about 3 μm to about 10 μm.

7. The system of claim 4 , wherein a thickness of the tissue is about 5 μm to about 7 μm.

8. The system of claim 1 , wherein the tissue sample is attached to a carrier substrate forming a sample-carrier construct, wherein said carrier substrate comprises agarose, amylose, amylopectin, alginate, gelatin, cellulose, polyolefin, polyethylene glycol, polyvinyl alcohol, and/or acrylate polymers and copolymers thereof.

9. The system of claim 8 , wherein the pistons comprise a piston head, wherein the piston head is configured to contact the carrier substrate.

10. The system of claim 9 , wherein a diameter of the piston head is less than a diameter of the piston.

11. The system of claim 1 , wherein the tissue sample is attached to a carrier substrate forming a sample-carrier construct, wherein said carrier substrate comprises agarose, agar, amylopectin, polyvinyl alcohol, Gellan gum, or alginate.

12. The system of claim 1 , wherein each receptacle is cylindrical, square, or rectangular.

13. The system of claim 1 , wherein the receiving array comprises an identifying feature at each receptacle, wherein the identifying feature uniquely identifies the receptacle.

Assignees

• Singular Genomics Systems Inc

Inventors

• Ishitsuka Yuji
• Koh Jaekyung
• Stover Robert James
• Shekhtmeyster Pavel
• Kovacs Sandor
• Cang Hu
• Rascon Valeria

Classifications

• G01N2001/364

  using resins, epoxy · CPC title

• G01N2001/315

  Basket-type carriers for tissues · CPC title

• G01N2001/305

  Fixative compositions · CPC title

• G01N2001/288

  Filter punches · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title