Chemical sensor device and method for manufacturing the same

Patent metadata

Field	Value
Publication number	US-12411133-B2
Application number	US-202117445486-A
Country	US
Kind code	B2
Filing date	Aug 19, 2021
Priority date	Mar 19, 2021
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

A chemical sensor device includes a sensor element; an oligopeptide mounted on a surface of the sensor element, the oligopeptide containing a first peptide sequence forming a β-sheet structure and a cysteine residue at a position different from the first peptide sequence; and a probe that is bonded to the cysteine residue and binds to or reacts with a specific substance.

First claim

What is claimed is:

1. A chemical sensor device comprising:
a sensor element having a surface;
an oligopeptide mounted on the surface of the sensor element, the oligopeptide containing a first peptide sequence forming a β-sheet structure and a cysteine residue at a position different from the first peptide sequence; and
a probe that is bonded to the cysteine residue and binds to or reacts with a specific substance,
the cysteine residue being located at either a C-terminal or an N-terminal of the oligopeptide, and
the probe being bonded to a sulfur atom of the cysteine residue via a succinimidyl group.

2. The device according to claim 1 , wherein
the first peptide sequence consists of a repeating sequence of a glycine residue and an alanine residue.

3. The device according to claim 1 , wherein
the first peptide sequence consists of a sequence containing a valine residue, an isoleucine residue, and a tyrosine residue.

4. The device according to claim 1 , wherein
the probe is bonded to the oligopeptide via a sulfur atom of the cysteine residue.

5. The device according to claim 1 , wherein
the probe is selected from a group consisting of a nucleic acid aptamer, a peptide aptamer, an enzyme, an antibody, a sugar chain, and a lectin.

6. The device according to claim 1 , wherein
the sensor element is a charge detection element containing graphene.

7. A chemical sensor device comprising:
a sensor element having a surface;
an oligopeptide mounted on the surface of the sensor element, the oligopeptide containing a first peptide sequence forming a β-sheet structure and a cysteine residue at a position different from the first peptide sequence; and
a probe that is bonded to the cysteine residue and binds to or reacts with a specific substance,
the probe being bonded to a sulfur atom of the cysteine residue via a succinimidyl group.

Assignees

• Toshiba Kk

Inventors

• Miki Hiroko
• Sugizaki Yoshiaki
• Isobayashi Atsunobu

Classifications

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title

• C07K19/00

  Hybrid peptides {, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes} · CPC title

• C07K17/00

  Carrier-bound or immobilised peptides (carrier-bound or immobilised enzymes C12N11/00); Preparation thereof · CPC title

• G01N27/4145

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• G01N33/5438

  Electrodes · CPC title