Cell treatment agent

Patent metadata

Field	Value
Publication number	US-12410395-B2
Application number	US-202117802478-A
Country	US
Kind code	B2
Filing date	Feb 27, 2021
Priority date	Feb 28, 2020
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

A cell treatment agent containing alginate sulfate as an active ingredient, and a set reagent for activating suspended or dormant cells, which is a combination reagent of the cell treatment agent and an activator containing polyvalent cations are provided.

First claim

The invention claimed is:

1. A cell treatment agent comprising alginate sulfate as an active ingredient and at least one selected from the group consisting of a cell culture solution, an extracellular fluid replacement solution, and a maintenance infusion,
wherein in the alginate sulfate, an alginate sulfate with a sulfate group introduced into a carbon atom at position 6 occupies more than 10% of a total of the alginate sulfate to achieve high treatment capacities for cells.

2. The cell treatment agent according to claim 1 , wherein an amount of the alginate sulfate with a sulfate group introduced into the carbon atom at position 6 is 20% or more of a total of the alginate sulfate.

3. The cell treatment agent according to claim 1 , wherein an amount of the alginate sulfate with a sulfate group introduced into the carbon atom at position 6 is 50% or more of a total of the alginate sulfate.

4. The cell treatment agent according to claim 1 , wherein an amount of the alginate sulfate with a sulfate group introduced into the carbon atom at position 6 is 80% or more of a total of the alginate sulfate.

5. A method for detaching a cell from a scaffold, wherein the scaffold is a living tissue or a culture substrate, and wherein the cell and scaffold are in a cell culture solution, the method comprising adding the cell treatment agent according to claim 1 to the cell culture solution.

6. A method for making a cell dormant, wherein the cell is in a tissue or in a cell culture, and the tissue or cell culture are in a cell culture solution, the method comprising adding the cell treatment agent according to claim 1 to the cell culture solution.

7. A method for maintaining viability of a cell, wherein the cell is in a tissue or in a cell culture, comprising providing the cell in the cell treatment agent according to claim 1 .

8. A method for maintaining a cell in an undifferentiated state, comprising providing the cell in the cell treatment agent according to claim 1 .

9. The cell treatment agent according to claim 1 , wherein the cell treatment agent is a liquid for cell preservation, tissue preservation, or organ preservation.

10. A set reagent for activation of a suspended cell or a dormant cell, the set reagent being a combination reagent of the cell treatment agent according to claim 1 and an activator containing a polyvalent cation.

Assignees

• Nagase Chemtex Corp

Inventors

• Hagiwara Akeo

Classifications

• C12N2539/10

  Coating allowing for selective detachment of cells, e.g. thermoreactive coating · CPC title

• C12N2533/74

  Alginate · CPC title

• C12N5/0018 Primary

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title