Automatic dishwashing detergent composition comprising an amylase

Patent metadata

Field	Value
Publication number	US-12410385-B2
Application number	US-202017066764-A
Country	US
Kind code	B2
Filing date	Oct 9, 2020
Priority date	Oct 24, 2019
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

An automatic dishwashing cleaning composition comprising a variant α-amylase wherein the variant α-amylase comprises amino acid substitution(s) selected from the group consisting of:
i) a mutation at position 91 and one or more mutation(s) at an amino acid residue at the base of the α-amylase TIM barrel structure, defined as residues 6, 7, 40, 96, 98, 100, 229, 230, 231, 262, 263, 285, 286, 287, 288, 322, 323, 324, 325, 362, 363 and 364; and/or ii) a mutation at position 172 and a mutation in position 288 or 324; and/or iii) Y364L
referring to SEQ ID NO: 1 for numbering.

First claim

What is claimed is:

1. An automatic dishwashing cleaning composition comprising a variant α-amylase,
wherein the variant α-amylase comprises amino acid substitution(s) selected from the group consisting of: a substitution at the position corresponding to position 91 of SEQ ID NO: 1 and a substitution at the position corresponding to position 40 of SEQ ID NO: 1,
wherein the variant α-amylase has α-amylase activity, and the amino acid sequence of the variant α-amylase has at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1; and
wherein the variant α-amylase comprises:
(i) substitutions selected from the group consisting of:
(a) X40N-X91R-X169H-X183M-X281N,
(b) X91R-X172R-X190P-X324M,
(c) X40N-X91R-X190P-X263Y,
(d) X40N-X91R-X244E-X364L,
(e) X91R-X172R-X190P-X324R,
(f) X91R-X116R-X172R-X244E-X281S-X288D,
(g) X40N-X91R-X100F-X116R-X172N-X244Q-X2818S,
(h) X40N-X91R-X172R-X244Q-X263Y-X281S,
(i) X91R-X172R-X190P-X324N, and
(j) X40D-X91R-X172R-X190P-X281S-X324R; and

(ii) pairwise deletions of residues selected from the group consisting of:
residues 181 and 182, and
residues 183 and 184,

wherein amino acid position numbering is relative to the amino acid sequence of SEQ ID NO: 1.

2. The composition according to claim 1 , wherein the substitution at the position corresponding to position 91 is S91R.

3. The composition according to claim 1 , wherein the substitution at the position corresponding to position 40 is X40N.

4. The composition according to claim 1 , wherein the substitution at the position corresponding to position 40 is selected from the group consisting of T40N and T40D.

5. The composition according to claim 1 , wherein the variant α-amylase further comprises a mutation at the position corresponding to position 172 of SEQ ID NO: 1.

6. The composition according to claim 5 , wherein the mutation at the position corresponding to position 172 of SEQ ID NO: 1 is a substitution with R or N.

7. The composition according to claim 1 , wherein the variant α-amylase further comprises mutations at the positions corresponding to positions 172 and 288 of SEQ ID NO: 1.

8. The composition according to claim 6 , wherein the variant α-amylase further comprises mutations at the positions corresponding to positions 116 and 281 of SEQ ID NO: 1.

9. The composition according to claim 1 , wherein the variant α-amylase further comprises mutation(s) at the position corresponding to position 190 and/or 244 of SEQ ID NO: 1.

10. The composition according to claim 1 , wherein the variant α-amylase comprises pairwise deletion of residues 181 and 182.

11. The composition according to claim 1 , wherein the variant α-amylase comprises:
pairwise deletions of residues selected from the group consisting of:
R181 and G182, and
T183 and G184.

12. The composition according to claim 1 , wherein the variant α-amylase comprises the following features:
(a) D or N at position 40 and/or R at position 91,
(b) N or R at position 172, and
(c) pairwise deletions at positions 181 and 182.

13. The composition according to claim 1 , further comprising a protease, wherein the amino acid sequence of the protease has at least 80% amino acid sequence identity with the amino acid sequence of SEQ ID NO: 5 or with the amino acid sequence of SEQ ID NO: 6, and wherein the protease comprises amino acid substitutions selected from the group consisting of:
(i) X198G/A/K/L/Q/R/T/V/S/L, X207Q, X211Q/N and X212Q in combination with at least three amino acid substitutions selected from the group consisting of: X039E, X074D, X099R, X126A, X127E and X128G;
(ii) X039E-X074D-X099R-X116R-X126A-X127E-X128G-X211Q;
(iii) X039E-X074D-X099R-X126A-X127E-X128G-X211N;
(iv) X039E-X074D-X099R-X126A-X127E-X128G-X211Q;
(v) X039E-X074D-X099R-X126A-X127E-X128G-X207Q;
(vi) any one of the proteases of (i), (ii), (iii), (iv), or (v) further comprising at least one amino acid substitution selected from X242D and X256E; and
(vii) X039E-X074D-X099R-X126A-X127E-X128G-X256E;
wherein amino acid position numbering is relative to the amino acid sequence of SEQ ID NO: 6.

14. The composition according to claim 1 , further comprising a protease, wherein the amino acid sequence of the protease has at least 90% amino acid sequence identity with the amino acid sequence of SEQ ID NO: 6, and wherein the protease comprises one or more, or two or more or three or more amino acid mutations selected from the group consisting of:
S3V, S9R, A13V, A11ST, G20*, L21F, 135V, N60D, V66A, N74D, S85N/R, S97SE, S97AD, S97D/G, S99G/M/D/E, S101A, V102E/I, G116V/R, S126F/L, P127Q, S128A, S154D, G157S, Y161A, R164S, A188P, V199I, Q200C/E/I/K/T/V/W/L, Y203W, N212D, M216S/F, A222V, Q239R/F, T249R, N255D, and L256E,N,Q,D.

15. The composition according to claim 1 , further comprising:
from 10% to 50% by weight of the composition of an organic complexing agent system; and
more than 10% by weight of the composition of a bleaching system, wherein the bleaching system comprises bleach and at least one of a bleach activator and a bleach catalyst.

16. The composition according to claim 1 , further comprising a bleach catalyst, wherein the bleach catalyst is a manganese bleach catalyst selected from the group consisting of 1,4,7-trimethyl-1,4,7-triazacyclononane (Me-TACN), 1,2,4,7-tetramethyl-1,4,7-triazacyclononane (Me/Me-TACN), and mixtures thereof.

17. A method of washing soiled dishware in a dishwasher comprising the steps of:
i) providing the soiled dishware;
ii) using a long hot program, having a main wash cycle lasting more than 15 minutes at a temperature of at least 40° C.;
iii) treating the soiled dishware with the cleaning composition according to claim 1 resulting in treated dishware; and
iv) rinsing the treated dishware.

18. An automatic dishwashing cleaning composition comprising:
a variant α-amylase, wherein the variant α-amylase comprises amino acid substitution(s) selected from the group consisting of:
a substitution at the position corresponding to position 91 of SEQ ID NO: 1 and a substitution at the position corresponding to position 40 of SEQ ID NO: 1,
wherein the variant α-amylase has α-amylase activity, and the amino acid sequence of the variant α-amylase has at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1

wherein amino acid position numbering of the variant α-amylase is relative to the amino acid sequence of SEQ ID NO: 1; and
a protease, wherein the amino acid sequence of the protease has at least 80% amino acid sequence identity with the amino acid sequence of SEQ ID NO: 5 or with the amino acid sequence of SEQ ID NO: 6 and wherein the protease comprises amino acid substitutions selected from the group consisting of:
(i) X198G/A/K/L/Q/R/T/V/S/L, X207Q, X211Q/N and X212Q in combination with at least three amino acid substitutions selected from the group consisting of: X039E, X074D, X099R, X126A, X127E and X128G;
(ii) X039E-X074D-X099R-X116R-X126A-X127E-X128G-X211Q;
(iii) X039E-X074D-X099R-X126A-X127E-X128G-X211N;
(iv) X039E-X074D-X099R-X126A-X127E-X128G-X211Q;
(v) X039E-X074D-X099R-X126A-X127E-X128G-X207Q;
(vi) any one of the proteases of (i), (ii), (iii), (iv), or (v) further comprising at least one amino acid substitution selected from X242D and X256E; and
(vii) X039E-X074D-X099R-X126A-X127E-X128G-X256E;
wherein amino acid position numbering of the protease is relative to the amino acid sequence of SEQ ID NO: 6.

19. An automatic dishwashing cleaning composition comprising a variant α-amyla

Assignees

• Procter & Gamble

Inventors

• Jackson Michelle
• Bell-Rusiewicz Katarzyna
• Bhate Manasi
• Chan Hon Kit
• Lassila Jonathan
• Paul Brian James
• Ramer Sandra W
• Tran Patricia

Classifications

• C11D2111/14

  Hard surfaces · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C12N9/2414

  Alpha-amylase (3.2.1.1.) · CPC title

• C11D3/3953

  Inorganic bleaching agents (C11D3/3951 takes precedence) · CPC title

• C11D3/3951

  combined with specific additives · CPC title