Natriuretic peptide receptor 1 antibodies and methods of use

Patent metadata

Field	Value
Publication number	US-12410255-B2
Application number	US-202318193581-A
Country	US
Kind code	B2
Filing date	Mar 30, 2023
Priority date	Jun 12, 2019
Publication date	Sep 9, 2025
Grant date	Sep 9, 2025

Abstract

This disclosure relates to anti-Natriuretic Peptide Receptor 1 (NPR1) antibodies including agonist antibodies which are able to activate the NPR1 receptor, pharmaceutical compositions comprising the same, and methods of treatment comprising the same.

First claim

The invention claimed is:

1. An isolated nucleic acid or nucleic acids encoding the amino acid sequence of an anti-NPR1 antibody or antigen binding fragment, wherein the antibody or antigen binding fragment comprises three heavy chain complementarity determining regions (HCDR1, HCDR2, and HCDR3) and three light chain complementarity determining regions (LCDR1, LCDR2, and LCDR3) selected from:

(I) 

SEQ ID NO: 28 (HCDR1),  

SEQ ID NO: 119 (HCDR2),

SEQ ID NO: 30 (HCDR3),  

SEQ ID NO: 41 (LCDR1), 

SEQ ID NO: 42 (LCDR2), and

SEQ ID NO: 134 (LCDR3);

(II) 

SEQ ID NO: 31 (HCDR1),  

SEQ ID NO: 119 (HCDR2), 

SEQ ID NO: 30 (HCDR3),

SEQ ID NO: 41 (LCDR1), 

SEQ ID NO: 42 (LCDR2), and 

SEQ ID NO: 134 (LCDR3);

(III)  

SEQ ID NO: 32 (HCDR1),

SEQ ID NO: 120 (HCDR2), 

SEQ ID NO: 30 (HCDR3), 

SEQ ID NO: 44 (LCDR1), 

SEQ ID NO: 45 (LCDR2), and 

SEQ ID NO: 135 (LCDR3); or

(IV) 

SEQ ID NO: 34 (HCDR1), 

SEQ ID NO: 121 (HCDR2), 

SEQ ID NO: 36 (HCDR3), 

SEQ ID NO: 47 (LCDR1), 

SEQ ID NO: 45 (LCDR2), and 

SEQ ID NO: 134 (LCDR3).

2. A vector comprising the isolated nucleic acid(s) of claim 1 .

3. A host cell comprising the isolated nucleic acid(s) of claim 1 .

4. A method of producing an isolated anti-NPR1 antibody or antigen binding fragment, comprising culturing the host cell of claim 3 under conditions suitable for producing the antibody or antigen binding fragment.

5. The isolated nucleic acid(s) of claim 1 , wherein the antibody or antigen binding fragment comprises:
(a) a heavy chain variable region comprising an amino acid sequence of SEQ ID NO: 201, and a light chain variable region comprising an amino acid sequence of SEQ ID NO: 136; or
(b) a heavy chain variable region comprising an amino acid sequence of SEQ ID NO: 122, and a light chain variable region comprising an amino acid sequence of SEQ ID NO: 136.

6. A vector comprising the isolated nucleic acid(s) of claim 5 .

7. A host cell comprising the isolated nucleic acid(s) of claim 5 .

8. A method of producing an isolated anti-NPR1 antibody or antigen binding fragment, comprising culturing the host cell of claim 7 under conditions suitable for producing the antibody or antigen binding fragment.

9. The isolated nucleic acid(s) of claim 1 , wherein the antibody or antigen binding fragment comprises:
(a) a heavy chain comprising an amino acid sequence of SEQ ID NO: 203, and a light chain comprising an amino acid sequence of SEQ ID NO: 138; or
(b) a heavy chain comprising an amino acid sequence of SEQ ID NO: 208, and a light chain comprising an amino acid sequence of SEQ ID NO: 138.

10. A vector comprising the isolated nucleic acid(s) of claim 9 .

11. A host cell comprising the isolated nucleic acid(s) of claim 9 .

12. A method of producing an isolated anti-NPR1 antibody or antigen binding fragment, comprising culturing the host cell of claim 11 under conditions suitable for producing the antibody or antigen binding fragment.

Assignees

• Novartis Ag

Inventors

• Diener John Louis
• Gadtke Lars
• Hartlepp Felix
• Hu Tiancen
• Ladetzki-Baehs Kathrin
• Romanowski Michael John
• Russo Cesare
• Wezler Xenia
• Xie Xiaoling

Classifications

• C12N15/63

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• A61P13/12

  of the kidneys · CPC title

• C07K2317/75

  Agonist effect on antigen · CPC title

• C07K2317/622

  Single chain antibody (scFv) · CPC title

• C07K2317/565

  Complementarity determining region [CDR] · CPC title