Method of manufacturing transfructosylation steviol glycosides using the lactobacillus mali
US-2020383364-A1 · Dec 10, 2020 · US
Composition for producing glucosylated steviol glycoside including glucosyltransferase and method of producing glucosylated steviol glycoside using the same
US12404532B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12404532-B2 |
| Application number | US-202017610352-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 8, 2020 |
| Priority date | Jun 14, 2019 |
| Publication date | Sep 2, 2025 |
| Grant date | Sep 2, 2025 |
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- Title
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Abstract
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The present disclosure relates to a composition for producing glucosylated steviol glycoside, the composition including glucosyltransferase including an amino acid sequence of SEQ ID NO: 1; and a method of producing glucosylated steviol glycoside using the same.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of producing a glucosylated steviol glycoside, the method comprising the step of contacting a steviol glycoside with a glucosyltransferase including an amino acid sequence of SEQ ID NO:1, a microorganism expressing the glucosyltransferase, or a culture of the microorganism. 2. The method of claim 1 , the method comprising the step of reacting a glucose donor and steviol glycoside in the presence of the glucosyltransferase including the amino acid sequence of SEQ ID NO: 1, the microorganism expressing the glucosyltransferase, or the culture of the microorganism. 3. The method of claim 2 , wherein the reacting is carried out at pH of 1 to 10. 4. The method of claim 2 , wherein the reacting is carried out at 1° C. to 80° C. 5. The method of claim 1 , wherein the glucosylated steviol glycoside is in the form in which glucose is linked to steviol glycoside via an α-(1,6) bond. 6. The method of claim 5 , wherein the glucosylated steviol glycoside is in the form in which glucose is linked via an α-(1,6) bond to glucose linked at 19-OH position of steviol glycoside. 7. The method of claim 1 , wherein the glucosylated steviol glycoside is in the form in which 1 to 3 glucose molecules are linked to steviol glycoside. 8. The method of claim 1 , wherein the steviol glycoside is one or more selected from the group consisting of Stevioside, Rubusoside, Dulcoside A, Rebaudioside A, Rebaudioside C, Rebaudioside D, Rebaudioside E, Rebaudioside F, and Rebaudioside M.
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Classifications
- C12P19/56Primary
having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin · CPC title
- C12P19/18Primary
produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title
Lactobacillus · CPC title
Hexosyltransferases (2.4.1) · CPC title
Hexosyltransferases (2.4.1) · CPC title
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Frequently asked questions
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- What does patent US12404532B2 cover?
- The present disclosure relates to a composition for producing glucosylated steviol glycoside, the composition including glucosyltransferase including an amino acid sequence of SEQ ID NO: 1; and a method of producing glucosylated steviol glycoside using the same.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P19/56. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 02 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).