Yeast with improved alcohol production

Patent metadata

Field	Value
Publication number	US-12398362-B2
Application number	US-201816619565-A
Country	US
Kind code	B2
Filing date	Jun 4, 2018
Priority date	Jun 6, 2017
Publication date	Aug 26, 2025
Grant date	Aug 26, 2025

Abstract

Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

First claim

What is claimed is:

1. Modified yeast cells derived from parental yeast cells, the modified cells comprising a genetic alteration that causes the modified cells to produce a decreased amount of functional Cpr1 polypeptide compared to the parental cells and a disruption of the YJL065c gene present in the parental cells that causes the modified cells to produce a decreased amount of functional Dls1 polypeptides compared to the parental cells, wherein the modified cells produce during fermentation an increased amount of ethanol compared to parental cells under equivalent fermentation conditions.

2. The modified cells of claim 1 , wherein the genetic alteration comprises a disruption of the YDR155c gene present in the parental cells.

3. The modified cells of claim 2 , wherein disruption of the YDR155c gene is the result of deletion of all or part of the YDR155c gene.

4. The modified cells of claim 2 , wherein disruption of the YDR155c gene is the result of deletion of a portion of genomic DNA comprising the YDR155c gene.

5. The modified cells of claim 2 , wherein disruption of the YDR155c gene is the result of mutagenesis of the YDR155c gene.

6. The modified cells of claim 1 , wherein the cells do not produce functional Cpr1 polypeptides.

7. The modified cells of claim 1 , wherein the cells do not produce Cpr1 polypeptides.

8. The modified cells of claim 1 , wherein the cells do not produce functional Dls1 polypeptides.

9. The modified cells of claim 1 , wherein the cells are of a Saccharomyces spp.

10. The modified cells of claim 1 , wherein the amount of ethanol produced by the modified yeast cells and the parental yeast cells is measured at 24 hours following inoculation of a hydrolyzed starch substrate comprising 34-35% dissolved solids and having a pH of 4.8-5.4.

11. A method for producing a modified yeast cell comprising: introducing a genetic alteration into a parental yeast cell, which genetic alteration reduces or prevents the production of functional Cpr1 polypeptide compared to the parental cells, thereby producing modified cells that produce during fermentation an increased amount of ethanol compared to the parental cells under equivalent fermentation conditions.

12. The method of claim 11 , wherein the genetic alteration comprises disrupting the YDR155c gene in the parental cells by genetic manipulation.

13. The method of claim 11 , wherein the genetic alteration comprises deleting the YDR155c gene in the parental cells using genetic manipulation.

14. The method of claim 11 , wherein disruption of the YDR155c gene is performed in combination with disrupting the YJL065 gene present in the parental cells.

15. The method of claim 11 , wherein the modified cell is from a Saccharomyces spp.

16. The method of claim 11 , wherein the amount of ethanol produced by the modified yeast cells and the parental yeast cells is measured at 24 hours following inoculation of a hydrolyzed starch substrate comprising 34-35% dissolved solids and having a pH of 4.8-5.4.

Assignees

• Danisco Us Inc

Inventors

• Payen Celia Emily Gaby
• Qi Min

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12N15/815

  for yeasts other than Saccharomyces · CPC title

• C12R2001/85

  Saccharomyces · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12N15/81 Primary

  for yeasts · CPC title