Method for extracting protein, starch and fiber from buckwheat

Patent metadata

Field	Value
Publication number	US-12398175-B2
Application number	US-201816611050-A
Country	US
Kind code	B2
Filing date	May 14, 2018
Priority date	May 16, 2017
Publication date	Aug 26, 2025
Grant date	Aug 26, 2025

Abstract

The present invention relates to a method for extracting protein, starch and fibers from buckwheat, more particularly from buckwheat groat or flour.

First claim

The invention claimed is:

1. A process for extracting buckwheat proteins, buckwheat starch and buckwheat fibers from a buckwheat flour or from a buckwheat groat, said buckwheat being common buckwheat ( Fagopyrum esculentum ), the process being free of organic solvents and organic reactants and comprising the steps of:
a) preparing an aqueous suspension from the buckwheat flour or from the buckwheat groat with a pH between 7 and 9, wherein the preparation of the aqueous suspension comprises a step of adding water to the buckwheat flour or to buckwheat groat in proportion of 4:1 (w/w) at a temperature comprised between room temperature and 50° C.,
b) fractionating the aqueous suspension by density, so as to obtain a light fraction (supernatant) comprising proteins, soluble carbohydrates and salts, and a heavy fraction (precipitate) comprising starch and fibers;
c) treating the light fraction (supernatant), so as to isolate the proteins; and
d) treating the heavy fraction (precipitate), so as to separate starch from fibers;
wherein when the process starts from buckwheat groat the step a) of preparation of the aqueous suspension comprises a step of wet grinding to obtain the buckwheat aqueous suspension with the buckwheat particles in said suspension having an average particle size of 100 μm, wherein when the process starts from buckwheat groat, the treating step of the light fraction c) comprises the following steps:
c1) adjusting the pH of the light fraction to the isoelectric pH of proteins, corresponding to a pH comprised in the range of 4 to 5, at a temperature comprised between room temperature and 50° C., so as to precipitate proteins;
c2) fractionating the light fraction by density, so as to separate the protein precipitate from a supernatant comprising soluble carbohydrates and salts;
c3) treating the protein precipitate at pH in the range of 6.5 to 7.5 at a temperature comprised between room temperature and 50° C., so as to neutralized and resolubilize proteins; and
c4) drying said proteins
wherein, when the process starts from buckwheat flour, the treating step of the light fraction c) comprises the following steps:

c′1) adjusting the pH of the light fraction to the isoelectric pH of proteins, corresponding to a pH comprised in the range of 4 to 5, at a temperature comprised between room temperature and 50° C., so as to precipitate proteins;
c′2) fractionating the light fraction by density, so as to obtain a protein precipitate and a supernatant comprising soluble carbohydrates and salts;
c′3) treating the protein precipitate at pH in the range of 6.5 to 7.5 at a temperature comprised between room temperature and 50° C., so as to neutralize and partially resolubilize the proteins to obtain an insoluble protein fraction and a soluble protein fraction;
c′4) separating by filtration the insoluble protein fraction from the soluble protein fraction;
c′5) drying the insoluble proteins; and
c′6) drying the soluble proteins,
wherein yield of buckwheat protein from said process is 2 to 10%, yield of buckwheat starch from said process is 45 to 60% and yield of buckwheat fiber from said process is less than 35%.

2. The process according to claim 1 , wherein the process is free of organic solvents and free of organic reactants.

3. The process according to claim 1 , wherein the treating step d) of the heavy fraction comprises the following steps:
d1) adding water to the heavy fraction at a temperature comprised between room temperature and 50° C., so as to resuspend the heavy fraction and to obtain a fiber fraction and a starch fraction;
d2) separating the fiber fraction from the starch fraction based at a temperature comprised between room temperature and 50° C.;
d3) drying the fibers;
d4) removing residual proteins in the starch fraction by density difference at pH 6-9 at a temperature comprised between room temperature and 50° C.; and
d5) drying the starch fraction.

4. The process according to claim 3 , wherein the treating step d4) of the starch fraction is repeated at least one time.

5. The process according to claim 3 , wherein the drying steps d3) and d5) of the treating step of the heavy fraction are carried out by using fluidized bed dryer, freeze dryer or hot air dryer.

6. The process according to claim 1 , wherein the drying steps c4), c′5) and c′6) of the treating step of the light fraction are carried out by using vacuum oven dryer, freeze dryer, or spray dryer.

7. The process according to claim 1 , wherein the separating steps (steps c′4) and d2)) by filtration are carried out by using sieve having size comprised between 100 and 125 μm.

8. The process according to claim 1 , wherein the fractionating step by density (steps b), c2), c′2) and d4)) includes a mechanical fractionating step, the mechanical fractionating step being carried out by using a horizontal screw decanter, a centrifugal decanter or a hydrocyclone.

9. The process according to claim 1 , wherein when the process starts from buckwheat groat, the treating step of the light fraction c1) comprises adjusting the pH of the light fraction to a pH value of 4.5 at a temperature comprised between room temperature and 50° C., so as to precipitate the proteins.

10. The process according to claim 1 , wherein when the process starts from buckwheat flour, the treating step of the light fraction c′1) comprises adjusting the pH of the light fraction to a pH value of 4.5 at a temperature comprised between room temperature and 50° C., so as to precipitate the proteins.

Assignees

• Roquette Freres

Inventors

• Hasjim Jovin
• Tao Jingling
• Pora Bernard

Classifications

• C08B30/02

  Preparatory treatment, e.g. crushing of raw materials {or steeping process (machines for preliminary washing A23N)} · CPC title

• C08B30/042 Primary

  from cereals or grains · CPC title

• C08B30/04 Primary

  Extraction or purification · CPC title

• C08B30/06

  Drying; Forming · CPC title

• C08B30/046

  from wheat · CPC title