Detection chip, method for using detection chip, and detection device

Patent metadata

Field	Value
Publication number	US-12397292-B2
Application number	US-202117622277-A
Country	US
Kind code	B2
Filing date	Feb 1, 2021
Priority date	Feb 20, 2020
Publication date	Aug 26, 2025
Grant date	Aug 26, 2025

Abstract

A detection chip, a method for using a dejection chip, and a detection device are provided. The detection chip includes a chip substrate and a first sealing film that are stacked. The chip substrate includes a first surface, and the first sealing film covers the first surface of the chip substrate. The chip substrate further includes a fluid channel on the first surface, and the fluid channel includes a plurality of membrane valve portions. The membrane valve portions are configured to allow a portion of fee first sealing film covering the membrane valve portions to approach and separate, so as to close and open the fluid channel correspondingly.

First claim

What is claimed is:

1. A detection chip, comprising: a chip substrate and a first sealing film that are stacked,
wherein the chip substrate comprises a first surface, and the first sealing film covers the first surface of the chip substrate;
the chip substrate further comprises a fluid channel on the first surface, and the fluid channel comprises a plurality of membrane valve portions; and
the membrane valve portions are configured to allow a portion of the first sealing film covering the membrane valve portions to approach and separate, so as to close and open the fluid channel correspondingly,
wherein the detection chip further comprises a membrane valve sealing plate,
wherein the membrane valve sealing plate is on a side of the first sealing film away from the chip substrate and comprises a plurality of protruding structures,
the plurality of protruding structures and the plurality of membrane valve portions are in one-to-one correspondence, and
in a case where the plurality of protruding structures are in contact with the plurality of membrane valve portions, respectively, the fluid channel is closed,
wherein the chip substrate further comprises at least one liquid cell,
the at least one liquid cell is communicated with the fluid channel,
at least one of the plurality of membrane valve portions is configured to close and open a portion of the fluid channel communicated with the at least one liquid cell,
wherein the fluid channel further comprises an extraction region and a plurality of first branches, and the at least one liquid cell comprises a plurality of liquid cells;
the plurality of first branches are communicated with the plurality of liquid cells in one-to-one correspondence, and each of the plurality of first branches is communicated with the extraction region;
the plurality of membrane valve portions comprise a plurality of first membrane valve portions respectively on the plurality of first branches, so as to control the plurality of first branches to be opened or closed;
the plurality of liquid cells are distributed in a circular shape around the extraction region,
wherein the fluid channel further comprises a reaction region and a plurality of second branches;
the reaction region is communicated with the extraction region and at least one of the plurality of liquid cells through the plurality of second branches, respectively;
the plurality of membrane valve portions further comprise a plurality of second membrane valve portions respectively on the plurality of second branches, so as to control the plurality of second branches to be opened or closed,
wherein the reaction region comprises a porous structure, the porous structure comprises a plurality of liquid containing holes, and the plurality of liquid containing holes are configured to contain identical or different amplification primers,
a position of one of the plurality of liquid containing holes serves as a center position, and remaining liquid containing holes among the plurality of liquid containing holes are distributed in a circular shape around the center position;
the detection chip further comprises a second sealing film,
wherein the chip substrate comprises a second surface opposite to the first surface, and the second sealing film covers the second surface of the chip substrate,
wherein the second sealing film is a composite film comprising metal foil and a polymer material that are stacked.

2. The detection chip according to claim 1 , wherein the first sealing film is an elastic film.

3. The detection chip according to claim 1 , wherein the plurality of liquid cells comprise a first liquid cell, a second liquid cell, a third liquid cell, a fourth liquid cell, and a fifth liquid cell,
the first liquid cell is configured to contain a lysis solution, the second liquid cell is configured to contain a first rinsing solution, the third liquid cell is configured to contain a second rinsing solution, the fourth liquid cell is configured to contain an eluent, and the fifth liquid cell is configured to accommodate a waste liquid generated in the extraction region during a reaction process.

4. The detection chip according to claim 1 , further comprising an adhesive layer,
wherein the adhesive layer is between the chip substrate and the first sealing film, and is configured to allow the chip substrate to be adhered with the first sealing film, and
the adhesive layer exposes the fluid channel of the chip substrate.

5. A detection device, adapted to operate a detection chip, wherein the detection chip comprises: a chip substrate and a first sealing film that are stacked,
the chip substrate comprises a first surface, and the first sealing film covers the first surface of the chip substrate;
the chip substrate further comprises a fluid channel on the first surface, and the fluid channel comprises a plurality of membrane valve portions; and
the membrane valve portions are configured to allow a portion of the first sealing film covering the membrane valve portions to approach and separate, so as to close and open the fluid channel correspondingly;
the detection device comprises a membrane valve control unit,
the membrane valve control unit is configured to mount the detection chip and comprises at least one protruding portion, and the at least one protruding portion is movable to control the portion of the first sealing film covering the membrane valve portions whether to approach the membrane valve portions or whether to separate from the membrane valve portions in a case where the detection chip is mounted on the membrane valve control unit, so as to close and open the fluid channel correspondingly,
wherein the detection chip further comprises a membrane valve sealing plate,
wherein the membrane valve sealing plate is on a side of the first sealing film away from the chip substrate and comprises a plurality of protruding structures,
the plurality of protruding structures and the plurality of membrane valve portions are in one-to-one correspondence, and
in a case where the plurality of protruding structures are in contact with the plurality of membrane valve portions, respectively, the fluid channel is closed,
wherein the chip substrate further comprises at least one liquid cell,
the at least one liquid cell is communicated with the fluid channel,
at least one of the plurality of membrane valve portions is configured to close and open a portion of the fluid channel communicated with the at least one liquid cell,
wherein the fluid channel further comprises an extraction region and a plurality of first branches, and the at least one liquid cell comprises a plurality of liquid cells;
the plurality of first branches are communicated with the plurality of liquid cells in one-to-one correspondence, and each of the plurality of first branches is communicated with the extraction region;
the plurality of membrane valve portions comprise a plurality of first membrane valve portions respectively on the plurality of first branches, so as to control the plurality of first branches to be opened or closed;
the plurality of liquid cells are distributed in a circular shape around the extraction region,
wherein the fluid channel further comprises a reaction region and a plurality of second branches;
the reaction region is communicated with the extraction region and at least one of the plurality of liquid cells through the plurality of second branches, respectively;
the plurality of membrane valve portions further comprise a plurality of second membrane valve portions respectively on the plurality of second branches, so as to control the plurality of second branches to be opened or closed,
wherein the reaction region comprises a porous structure, the porous structure comprises a plurality of liquid containing holes,

Assignees

• Beijing Boe Health Tech Co Ltd
• Boe Technology Group Co Ltd

Inventors

• Hu Lijiao
• Cui Haochen
• Yuan Chungen
• Hu Tao
• Li Jing
• Gan Weiqiong
• Shen Xiaohe

Classifications

• B01L2400/0638

  membrane valves, flap valves · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2300/049

  Valves integrated in closure · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title