Determination of magnesium body concentration

Patent metadata

Field	Value
Publication number	US-12391990-B2
Application number	US-202017621996-A
Country	US
Kind code	B2
Filing date	Jun 24, 2020
Priority date	Jun 24, 2019
Publication date	Aug 19, 2025
Grant date	Aug 19, 2025

Abstract

The presently-disclosed subject matter includes methods for detecting methylation biomarkers in a biological sample from a subject in need of assessment for magnesium deficiency status, methods of diagnosing magnesium deficiency, methods of diagnosing magnesium insufficiency, methods of treating magnesium deficiency, methods of diagnosing magnesium insufficiency, and methods of preventing or reducing a risk of developing a condition linked to magnesium deficiency.

First claim

What is claimed is:

1. A method for predicting and treating magnesium deficiency in a subject, said method comprising:
a) detecting methylation at CpG sites in DNA obtained from a biological sample from the subject, wherein the subject is in need of assessment for magnesium deficiency status; and
b) identifying the subject as being in need of magnesium supplementation when methylation is found at the CpG site: cg00430271 or cg25731074; and
c) administering an effective amount of magnesium to the identified subject.

2. The method of claim 1 , and further comprising detecting 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) methylation in the DNA obtained from the biological sample from the subject and differentiating between 5-hmC and 5-mC methylation in the sample.

3. The method of claim 1 , further comprising detecting 5-hmC and 5-mC methylation, 5-hmC methylation, or 5-mC methylation by performing bisulfite (BS) treatment of the DNA in the biological sample.

4. The method of claim 2 , further comprising differentiating between 5-hmC and 5-mC methylation in the biological sample using a TAB-Seq and TAB-Array protocol.

5. The method of claim 1 , and further comprising determining for the subject a magnesium tolerance test (MTT) score, where an MTT score of greater than or equal to 50% is further indicative of a magnesium deficiency, an MTT score of less than 50% and greater than or equal to 25% is further indicative of a magnesium insufficiency, and an MTT score of less than 25% is further indicative of a magnesium sufficiency.

6. The method of claim 1 , and further comprising identifying the subject as being in need of magnesium supplementation when methylation is found at the CpG sites comprising: cg00430271 and cg25731074.

7. The method of claim 6 , and further comprising administering an effective amount of magnesium to the identified subject.

8. The method of claim 1 , wherein the biological sample includes blood leukocyte DNA.

9. The method of claim 1 , and further comprising identifying the subject as being in need of magnesium supplementation when methylation is found at the CpG sites comprising: cg00430271, cg11840205, and cg25731074.

10. The method of claim 9 , and further comprising administering an effective amount of magnesium to the identified subject.

11. The method of claim 1 , and further comprising identifying the subject as being in need of magnesium supplementation when methylation is found at the CpG sites comprising: cg00430271, cg04386563, cg11840205, and cg25731074.

12. The method of claim 11 , and further comprising administering an effective amount of magnesium to the identified subject.

13. The method of claim 1 , and further comprising determining Ca:Mg ratio of the diet of the subject, and selecting the effective amount of magnesium to decrease the Ca:Mg ratio.

Assignees

• Univ Vanderbilt

Inventors

• Dai Qi
• Yu Chang
• Shrubsole Martha
• Zhu Xiangzhu
• Seidner Douglas

Classifications

• C12Q2600/154

  Methylation markers · CPC title

• A61K33/06

  Aluminium, calcium or magnesium; Compounds thereof {, e.g. clay} · CPC title

• G16B20/00

  ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations · CPC title

• C12Q1/6883 Primary

  for diseases caused by alterations of genetic material · CPC title