Spatial control of polynucleotide synthesis by strand capping

Patent metadata

Field	Value
Publication number	US-12391970-B2
Application number	US-202017095650-A
Country	US
Kind code	B2
Filing date	Nov 11, 2020
Priority date	Nov 11, 2020
Publication date	Aug 19, 2025
Grant date	Aug 19, 2025

Abstract

Enzymatic polynucleotide synthesis with a template-independent polymerase is used to create multiple polynucleotides having different, arbitrary sequences on the surface of an array. The array provides a spatially-addressable substrate for solid-phase synthesis. Blocking groups are attached to the 3′ ends of polynucleotides on the array. Prior to polynucleotide extension, the blocking groups are removed at a selected location on the array. In an implementation, the blocking groups are acyl groups removed with a negative voltage created at an electrode. The array is then incubated with the polymerase and a single species of nucleotide. Nucleotides are incorporated onto the 3′ ends of the polynucleotides without blocking groups. Washing removes the polymerase and free nucleotides. To create polynucleotides with different sequences at different locations on the array, the location where the blocking groups are removed and the species of nucleotide may be changed during repeated cycles of synthesis.

First claim

The invention claimed is:

1. A method for enzymatic synthesis of polynucleotides, the method comprising:
on a microelectrode array having a plurality of initiators attached thereto,
a) designing a sequence of nucleotide bases that encodes digital information;
b) selectively removing 3′ blocking groups from the initiators at a selected location on the microelectrode array by activating a subset of individually addressable electrodes in the microelectrode array, wherein selectively removing the 3′ blocking groups comprises creating a localized basic environment at the selected location and wherein the localized basic environment is created by a voltage generated at an electrode;
c) incubating the microelectrode array with a single species of nucleotide and template-independent polymerase;
d) contacting the microelectrode array with a wash solution; and
e) repeating steps b)-d) until the sequence of nucleotide bases is synthesized at the selected location.

2. The method of claim 1 , further comprising, prior to step a), attaching the 3′ blocking groups to the plurality of initiators on the array.

3. The method of claim 2 , further comprising, after attaching the 3′ blocking groups to the plurality of initiators and prior to step a), washing the array with a second wash solution.

4. The method of claim 2 , wherein attaching the 3′ blocking groups to the plurality of initiators on the array comprises acylating 3′—OH groups on the plurality of initiators.

5. The method of claim 4 , wherein acylating the 3′—OH groups on the plurality of initiators comprises incubating the array with a solution comprising acyl imidazole and a buffer.

6. The method of claim 1 , further comprising repeating steps (b-d) while changing both the selected location and the species of nucleotide each at least once.

7. The method of claim 1 , wherein the single species of nucleotide comprises unmodified nucleotides and incubating the array with the single species of nucleotide and the template-independent polymerase is performed for a reaction time.

8. The method of claim 1 , wherein the 3′ blocking groups comprise acyl groups and selectively removing the 3′ blocking groups comprises deacylating the initiators.

9. The method of claim 1 , wherein the localized basic environment is created by a voltage generated at an electrode and the voltage is about −1.4 to −2.0 V.

10. The method of claim 1 , wherein the template-independent polymerase is a modified template independent polymerase capable of incorporating 3′-OH modified nucleotides and the single species of nucleotide comprises 3′-OH modified nucleotides.

11. The method of claim 10 , wherein the 3′-OH modified nucleotides comprise 3′-O-acetyl modified nucleotides.

12. The method of claim 1 , wherein the single species of nucleotide comprises nucleotides tethered to the template-independent polymerase.

13. The method of claim 1 , prior to step b), contacting the array with a deacylation solution.

14. The method of claim 13 , wherein the deacylation solution comprises a buffer at a pH between about 4-10.

15. A method for enzymatic synthesis of polynucleotides, the method comprising:
on a microelectrode array having a plurality of initiators attached thereto,
a) selectively removing 3′ blocking groups from the initiators at the selected location on the microelectrode array by activating a subset of individually addressable electrodes in the microelectrode array, wherein selectively removing the 3′ blocking groups comprises creating a localized basic environment at the selected location and wherein the localized basic environment is created by a voltage generated at an electrode;
b) incubating the microelectrode array with a single species of nucleotide and template-independent polymerase; and
c) contacting the microelectrode array with a wash solution,

 further comprising repeating steps a)-c) while changing both the selected location and the species of nucleotide each at least once, wherein the repeating steps a)-c) synthesizes a first polynucleotide at a first location on the array and a second polynucleotide at a second location on the array each with different sequences encoding different digital information.

Assignees

• Microsoft Technology Licensing Llc

Inventors

• Nguyen Bichlien Hoang
• Smith Jake

Classifications

• C12Y207/07031

  DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase · CPC title

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• C12N9/1264

  DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase · CPC title

• Y02E60/36

  Hydrogen production from non-carbon containing sources, e.g. by water electrolysis · CPC title

• C25B15/02

  Process control or regulation · CPC title