Plant having enhanced resistance against colorado potato beetle and method for producing same, and method for evaluating resistance against colorado potato beetle in plant

Patent metadata

Field	Value
Publication number	US-12391955-B2
Application number	US-202017905518-A
Country	US
Kind code	B2
Filing date	Mar 3, 2020
Priority date	Mar 3, 2020
Publication date	Aug 19, 2025
Grant date	Aug 19, 2025

Abstract

The spirosolane skeleton 23-position hydroxylase gene and the spirosolane skeleton 23-position acetyltransferase gene derived from S. chacoense, S. tuberosum , and S. lycopersicum are found to be involved with the biosynthesis of leptine, which achieves resistance against Colorado potato beetle.

First claim

The invention claimed is:

1. A transformed cell that is capable of regenerating Solanum tuberosum having increased resistance against Colorado potato beetle, the transformed cell comprising:
(a) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6; and
(b) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12.

2. A transformed Solanum tuberosum plant having increased resistance to Colorado potato beetle, which is regenerated from the transformed cell according to claim 1 .

3. A method for producing a Solanum tuberosum plant having Colorado potato beetle resistance, the method comprising:
introducing, to a cell, at least one DNA selected from the group consisting of (a) to (b) below and at least one DNA selected from the group consisting of (c) to (d) below; and
regenerating a Solanum tuberosum plant from a transformed cell to which the DNA selected from (a) to (b) and the DNA selected from (c) to (d) is introduced, wherein the DNAs are:
(a) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6;
(b) DNA encoding a protein that consists of an amino acid sequence having 80% or higher identity to an amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6, and has an activity to hydroxylate position 23 of a spirosolane skeleton;
(c) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12; and
(d) DNA encoding a protein that consists of an amino acid sequence having 80% or higher identity to an amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12, and has an activity to acetylate a hydroxy group of position 23 of a spirosolane skeleton.

4. A method for producing a Solanum tuberosum plant having resistance against Colorado potato beetle, the method comprising:
crossing a Solanum tuberosum plant having at least one DNA selected from the group consisting of (a) to (b) below, with a Solanum tuberosum plant having at least one DNA selected from the group consisting of (c) to (d) below;
determining the resistance against Colorado potato beetle in a Solanum tuberosum plant obtained in the crossing, and
selecting a Solanum tuberosum plant that is determined to have the resistance to Colorado potato beetle, wherein the DNAs are
(a) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6;
(b) DNA encoding a protein that consists of an amino acid sequence having 80% or higher identity to an amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6, and has an activity to hydroxylate position 23 of a spirosolane skeleton;
(c) DNA encoding a protein that consists of an amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12; and
(d) DNA encoding a protein that consists of an amino acid sequence having 80% or higher identity to an amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12, and has an activity to acetylate a hydroxy group of position 23 of a spirosolane skeleton.

5. The transformed cell that is capable of regenerating into a Solanum tuberosum plant having increased resistance against Colorado potato beetle according to claim 1 ,
wherein the transformed cell has 1.1 times or higher expression of endogenous DNA-of (a) compared to an untransformed plant cell or has 1.1 times or higher expression of RNA corresponding to (a) compared to an untransformed plant cell, and
the transformed cell has 1.1 times or higher expression of endogenous DNA of (b) compared to the untransformed plant cell or has 1.1 times or higher expression of RNA corresponding to (b) compared to the untransformed plant cell.

6. The production method according to claim 3 ,
wherein the Solanum tuberosum plant has an increased accumulation amount of leptine.

Assignees

• Kaneka Corp
• Univ Kobe Nat Univ Corp
• Nat Agriculture & Food Res Org
• Riken

Inventors

• Umemoto Naoyuki
• Mizutani Masaharu
• Akiyama Ryota
• Asano Kenji
• HAMADA Haruyasu
• Nagira Yozo
• Endo Akira
• Saito Kazuki

Classifications

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• A01H1/127

  for insect resistance · CPC title

• A01H6/827

  Solanum tuberosum [potato] · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C07K14/415

  from plants · CPC title