Method and system of sensitizing cancer cells to chemical treatment by plasma based activation

Patent metadata

Field	Value
Publication number	US-12383753-B2
Application number	US-201915734149-A
Country	US
Kind code	B2
Filing date	May 31, 2019
Priority date	Jun 1, 2018
Publication date	Aug 12, 2025
Grant date	Aug 12, 2025

Abstract

A method and system of adaptive cold atmospheric based treatment for diseased tissues, such as an area with cancerous cells, is disclosed. A gas such as helium is ionized via supplying power between and anode and a cathode to create an initial cold atmospheric plasma jet. The initial plasma jet is directed toward the area for a sufficient time, such as 10 seconds, to sensitize the cells via a reactive species. A reactive treatment, such as H2O2 or TMZ, is directed to the sensitized cells. The effectiveness of the reactive treatment is increased by the process of sensitizing the cells.

First claim

What is claimed is:

1. A system for sensitization treatment of an area having cancerous cells and normal cells, comprising:
a plasma device to generate a cold atmospheric plasma jet between an anode and a cathode, the generated plasma jet directed at the area having cancerous cells and normal cells;
a controller coupled to a power supply and a gas supply to control power to the anode and cathode, and gas supply to the plasma device, the controller activating the cold atmospheric plasma jet for a sufficient time to sensitize the cells via a reactive species, the controller deactivating the cold atmospheric jet after the sufficient time; and
a treatment device distinct from the plasma device operable to apply a reactive treatment to the sensitized cells in addition to the reactive species generated from the plasma jet after the cells are sensitized by the cold atmospheric plasma jet and the cold atmospheric plasma jet is deactivated.

2. The system of claim 1 , wherein the sensitized cells are the cancerous cells.

3. The system of claim 2 , wherein the cancerous cells are one of brain cancer cells, breast cancer cells, pancreatic cancer cells, skin cancer cells, bladder cancer cells, colon cancer cells, or lung cancer cells.

4. The system of claim 1 , wherein the reactive treatment includes one of a solution of H2O2, or radiation.

5. The system of claim 1 , wherein the controller is operative to change gas flow or gas composition generating the plasma jet.

6. The system of claim 1 , wherein the controller is operative to change discharge voltage or discharge frequency generating the plasma jet.

7. The system of claim 1 , wherein the gas supply supplies helium to the plasma device at a flow rate between 10-17 liters per minute.

8. The system of claim 1 , wherein the discharge voltage of the plasma jet is between 2 and 5 kV and the discharge frequency of the plasma jet is between 10-50 KHz.

9. The system of claim 1 , wherein the sufficient time is greater than 10 seconds.

10. The system of claim 1 , wherein the reactive treatment is chemotherapy, and wherein the treatment device is operable to be adjusted to reduce the reactive treatment below a baseline level in response to the sensitized cells.

11. A method of treating an area having cells, the method comprising:
ionizing a gas via supplying electrical power between an anode and a cathode to create a cold atmospheric plasma jet from a plasma jet device;
directing the cold atmospheric plasma jet toward the area for a sufficient time to sensitize the cells via a reactive species;
deactivating the cold atmospheric plasma jet after the sufficient time; and
directing a reactive treatment, in addition to the reactive species generated from the plasma jet, to the sensitized cells from a treatment device distinct from the plasma jet device after the cold atmospheric plasma jet is deactivated.

12. The method of claim 11 , wherein the selected cells are cancerous cells.

13. The method of claim 11 , wherein the cancerous cells are one of brain cancer cells, breast cancer cells, pancreatic cancer cells, skin cancer cells, bladder cancer cells, colon cancer cells, or lung cancer cells.

14. The method of claim 11 , wherein the reactive treatment includes one of a solution of H2O2, or radiation.

15. The method of claim 11 , wherein the gas is helium, and the helium is supplied at a flow rate between 10-17 liters per minute.

16. The method of claim 11 , wherein the discharge voltage of the plasma jet is between 2 and 5 kV and the discharge frequency of the plasma jet is between 10-50 KHz.

17. The method of claim 11 , wherein the sufficient time is greater than 10 seconds.

18. The method of claim 11 , further comprising reducing the reactive treatment below a baseline level to account for the sensitized cells.

19. The method of claim 1 , wherein the reactive treatment is Temozolomide (TMZ).

20. The method of claim 14 , wherein the reactive treatment is Temozolomide (TMZ).

Assignees

• Univ George Washington
• Univ George Washington

Inventors

• Yan Dayun
• Keidar Michael
• Gjika Eda

Classifications

• H01J37/32825

  Working under atmospheric pressure or higher · CPC title

• H01J37/32449

  Gas control, e.g. control of the gas flow · CPC title

• H01J37/32366

  Localised processing · CPC title

• A61K41/00

  Medicinal preparations obtained by treating materials with wave energy or particle radiation {; Therapies using these preparations} · CPC title

• A61K33/40

  Peroxides · CPC title