Pharmaceutical composition and cosmetic composition

Patent metadata

Field	Value
Publication number	US-12383583-B2
Application number	US-202418734602-A
Country	US
Kind code	B2
Filing date	Jun 5, 2024
Priority date	Nov 7, 2018
Publication date	Aug 12, 2025
Grant date	Aug 12, 2025

Abstract

Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.

First claim

What is claimed is:

1. A method for activating secretion of a growth factor of hair papilla cells, comprising:
applying to scalp or hair a supernatant of a culture medium,
wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

2. The method of claim 1 , wherein the somatic cells comprise at least one cell selected from the group consisting of a blood cell and a fibroblast.

3. A method for promoting production of a fibroblast growth factor family for hair papilla cells, comprising:
applying to scalp or hair a supernatant of a culture medium,
wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

4. The method of claim 3 , wherein the somatic cells comprise at least one cell selected from the group consisting of a blood cell and a fibroblast.

5. A method for promoting production of a vascular endothelial cell growth factor for hair papilla cells, comprising:
applying to scalp or hair a supernatant of a culture medium,
wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

6. The method of claim 5 , wherein the somatic cells comprise at least one cell selected from the group consisting of a blood cell and a fibroblast.

7. A method for activating secretion of IGF-1 for hair papilla cells, comprising:
applying to scalp or hair a supernatant of a culture medium,
wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

8. The method of claim 7 , wherein the somatic cells comprise at least one cell selected from the group consisting of a blood cell and a fibroblast.

9. A method for improving growth of hair papilla cells, comprising:
contacting the hair papilla cells with a composition, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agent, a sedative, a stabilizer, a preservative, an antiseptic, a surfactant, a humectant, a thickener, a natural fat or oil, a skin whitener, a chelating agent, silicon, an antiseptic agent, and a microbicide, wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

10. A method for activating secretion of a growth factor of hair papilla cells, comprising:
applying a composition to scalp or hair, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agent, a sedative, a stabilizer, a preservative, an antiseptic, a surfactant, a humectant, a thickener, a natural fat or oil, a skin whitener, a chelating agent, silicon, an antiseptic agent, and a microbicide, wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

11. A method for promoting production of a fibroblast growth factor family for hair papilla cells, comprising:
applying a composition, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agent, a sedative, a stabilizer, a preservative, an antiseptic, a surfactant, a humectant, a thickener, a natural fat or oil, a skin whitener, a chelating agent, silicon, an antiseptic agent, and a microbicide, wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced to scalp or hair, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agent, a sedative, a stabilizer, a preservative, an antiseptic, a surfactant, a humectant, a thickener, a natural fat or oil, a skin whitener, a chelating agent, silicon, an antiseptic agent, and a microbicide, wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

12. A method for promoting production of a vascular endothelial cell growth factor for hair papilla cells, comprising:
applying a composition to scalp or hair, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agent, a sedative, a stabilizer, a preservative, an antiseptic, a surfactant, a humectant, a thickener, a natural fat or oil, a skin whitener, a chelating agent, silicon, an antiseptic agent, and a microbicide, wherein the supernatant of the culture medium is produced by a method comprising culturing somatic cells, introducing genes encoding reprogramming factors into the somatic cells to produce reprogrammed somatic cells, inducing iPS cells (induced pluripotent stem cells) from the reprogrammed somatic cells, and subsequently recovering the supernatant of the culture medium in which the iPS cells were induced.

13. A method for activating secretion of IGF- 1 for hair papilla cells, comprising:
applying a composition, wherein the composition comprising a supernatant of a culture medium; and at least one selected from the group consisting of a buffering agent, an emulsifier, a suspending agen

Assignees

• Tanabe Koji
• I Peace Inc

Inventors

• Tanabe Koji
• Suto Kenta

Classifications

• A61Q19/08

  Anti-ageing preparations · CPC title

• A61K8/982

  Reproductive organs; Embryos, Eggs · CPC title

• A61P17/14 Primary

  for baldness or alopecia · CPC title

• A61Q7/00

  Preparations for affecting hair growth · CPC title

• A61K35/545 Primary

  Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells · CPC title