Methods of selectively modulating gastrointestinal microbial growth

Patent metadata

Field	Value
Publication number	US-12383570-B2
Application number	US-202418420540-A
Country	US
Kind code	B2
Filing date	Jan 23, 2024
Priority date	Nov 8, 2018
Publication date	Aug 12, 2025
Grant date	Aug 12, 2025

Abstract

Methods of feeding animals are disclosed wherein providing feed additives are provided that modulate the gut microbiome to improve the health, nutrition, and growth performance. Methods of modulating the microbial species present in the gastrointestinal tract of an animal are also disclosed. Such modulation includes, for example, modulating the level or function of microbial species.

First claim

What is claimed is:

1. A method of modulating the growth of at least one bacterial species in the gastrointestinal tract of a bird, said method comprising: administering a nutritional composition comprising a base nutritional composition and a synthetic oligosaccharide preparation to a bird, wherein said synthetic oligosaccharide preparation comprises at least n fractions of oligosaccharides each having a distinct degree of polymerization selected from 1 to n (DP1 to DPn fractions), wherein n is an integer greater than 3; and wherein each of a DP1 and DP2 fraction independently comprises from about 0.5% to about 15% of anhydro-subunit containing oligosaccharides by relative abundance as determined by mass spectrometry, wherein a level of at least one bacterial species in a gastrointestinal sample from said bird is increased or decreased relative to a level of said at least one bacterial species in a gastrointestinal sample from said bird prior to said administering said nutritional composition to said bird, and wherein said at least one bacterial species is selected from the group consisting of Odoribacter splanchnicus, Bacteroides dorei, Barnesiella intestinthominis, Bacillus clarus, Butyricimonas faecalis, Oscillibacter spp., Campylobacter jejuni, Helicobacter pullorum, Campylobacter coli , and Escherichia coli.

2. A method of modulating the growth of at least one bacterial species in the gastrointestinal tract of a bird, said method comprising: administering a nutritional composition comprising a base nutritional composition and a synthetic oligosaccharide preparation to a bird, wherein said synthetic oligosaccharide preparation comprises at least n fractions of oligosaccharides each having a distinct degree of polymerization selected from 1 to n (DP1 to DPn fractions), wherein n is an integer greater than 3; and wherein each of a DP1 and DP2 fraction independently comprises from about 0.5% to about 15% of anhydro-subunit containing oligosaccharides by relative abundance as determined by mass spectrometry, wherein a level of at least one bacterial species in a gastrointestinal sample from said bird is increased or decreased relative to a level of said at least one bacterial species in a gastrointestinal sample from a comparable control bird, that has been administered a comparable nutritional composition lacking said synthetic oligosaccharide preparation, and wherein said at least one bacterial species is selected from the group consisting of Odoribacter splanchnicus, Bacteroides dorei, Barnesiella intestinthominis, Bacillus clarus, Butyricimonas faecalis, Oscillibacter spp., Campylobacter jejuni, Helicobacter pullorum, Campylobacter coli , and Escherichia coli.

3. The method of claim 2 , wherein said method comprises promoting the growth of said at least one bacterial species, and wherein said level of at least one bacterial species in said gastrointestinal sample is increased relative to a level of said at least one bacterial species in a gastrointestinal sample from a comparable control bird, that has been administered a comparable nutritional composition lacking said synthetic oligosaccharide preparation.

4. A method of modulating expression of at least one bacterial protein within the gastrointestinal tract of a bird, said method comprising: administering a nutritional composition comprising a base nutritional composition and a synthetic oligosaccharide preparation to a bird, wherein said synthetic oligosaccharide preparation comprises at least n fractions of oligosaccharides each having a distinct degree of polymerization selected from 1 to n (DP1 to DPn fractions), wherein n is an integer greater than 3; and wherein each of a DP1 and DP2 fraction independently comprises from about 0.5% to about 15% of anhydro-subunit containing oligosaccharides by relative abundance as determined by mass spectrometry, wherein a level of at least one bacterial protein in a gastrointestinal sample is increased or decreased relative to a level of said at least one bacterial protein in a gastrointestinal sample from said bird prior to administration of said nutritional composition to said bird, and wherein said at least one bacterial protein is selected from the group consisting of CAZyme a-L-arabinofuranosidase (GH127), outer membrane receptor associated with the starch utilization system (susC), and starch binding protein (susD).

5. A method of modulating expression of at least one bacterial protein within the gastrointestinal tract of a bird, the method comprising: administering a nutritional composition comprising a base nutritional composition and a synthetic oligosaccharide preparation to a bird, wherein said synthetic oligosaccharide preparation comprises at least n fractions of oligosaccharides each having a distinct degree of polymerization selected from 1 to n (DP1 to DPn fractions), wherein n is an integer greater than 3; and wherein each of a DP1 and DP2 fraction independently comprises from about 0.5% to about 15% of anhydro-subunit containing oligosaccharides by relative abundance as determined by mass spectrometry, and wherein a level of at least one bacterial protein in a gastrointestinal sample is increased or decreased relative to a level of said at least one protein in a gastrointestinal sample from a comparable control bird, that has been administered a comparable nutritional composition lacking said synthetic oligosaccharide preparation, and wherein said at least one bacterial protein is selected from the group consisting of CAZyme a-L-arabinofuranosidase (GH127), outer membrane receptor associated with the starch utilization system (susC), and starch binding protein (susD).

6. The method of claim 4 , wherein said level of said at least one bacterial protein in said gastrointestinal sample is decreased relative to said level of said at least one bacterial protein in a gastrointestinal sample from said bird prior to administration of said nutritional composition to said bird.

7. The method of claim 5 , wherein said level of said at least one bacterial protein in said gastrointestinal sample is decreased relative to said level of said at least one bacterial protein in a gastrointestinal sample from said a comparable control bird prior to administration of said nutritional composition to said bird.

8. The method of claim 4 , wherein said bacterial protein is CAZyme a-L-arabinofuranosidase (GH127).

9. The method of claim 5 , wherein said bacterial protein is CAZyme a-L-arabinofuranosidase (GH127).

Assignees

• Dsm Ip Assets Bv

Inventors

• Duval Stephane
• Geremia John Michael
• Laprade Lisa Ann
• Waguespack-Levy April
• Perez Calvo Estefania
• Schyns Ghislain
• Verlhac Viviane
• Claypool Joshua Thomas

Classifications

• A23K50/75

  for poultry · CPC title

• A23K50/30

  for swines · CPC title

• A23K20/163 Primary

  Sugars; Polysaccharides · CPC title

• Y02P60/50

  Livestock or poultry management · CPC title

• A23K50/00

  Feeding-stuffs specially adapted for particular animals · CPC title