Scale up synthesis of silicasome nanocarriers

Patent metadata

Field	Value
Publication number	US-12383499-B2
Application number	US-201816235950-A
Country	US
Kind code	B2
Filing date	Dec 28, 2018
Priority date	Jan 1, 2018
Publication date	Aug 12, 2025
Grant date	Aug 12, 2025

Abstract

In order to facilitate the approval and commercialization of silicasome drug delivery systems (e.g. irinotecan silicasomes) it is necessary to scale up synthesis of the drug-loaded silicasomes. In this regard, it was discovered that the synthesis protocols used for laboratory synthesis of drug-loaded silicasomes (e.g., 500 mg/batch) do not scale to large scale silicasome production, because the resulting products were too heterogeneous for use as pharmaceuticals. Accordingly, new methods are provided herein that effectively afford the large-scale production of mesoporous silica nanoparticles (MSNPs) and lipid bilayer coated MSNPs (silicasomes).

First claim

What is claimed is:

1. A method for large-scale preparation of mesoporous silica nanoparticles suitable for use in pharmaceuticals, said method comprising:
providing a cationic surfactant in water at a concentration greater than a critical micellar concentration (CMC) of said cationic surfactant to form an aqueous mixture of micelles, where said cationic surfactant comprises cetyltrimethylammonium chloride (CTAC) or cetyltrimethylammonium bromide (CTAB);
adding triethanolamine (TEA) and tetraethylorthosilicate (TEOS) to said aqueous mixture of micelles at a molar ratio of water: cationic surfactant: TEA:TEOS ranging from 100 to 150 water: 0.06 to 0.10 cationic surfactant:0.04 to 0.08 TEA: 0.08 to 1.2 TEOS; and
stirring or agitating said aqueous mixture of micelles to allow said cationic surfactant, said TEA, and said TEOS in said aqueous mixture of micelles to react to form a population of mesoporous silica nanoparticles (MSNPs), wherein said method produces at least 30 grams of mesoporous silica nanoparticles in a single batch, and
wherein the providing of the cationic surfactant at the concentration greater than the CMC maintains a size of MSNPs in presence of said TEA.

2. The method of claim 1 , wherein said cationic surfactant is CTAC.

3. The method of claim 1 , wherein said method further comprises adding ethanol to said aqueous mixture of micelles after said population of MSNPs is formed to precipitate said population of MSNPs.

4. The method of claim 1 , wherein the molar ratio of water:cationic surfactant:TEA:TEOS is 125:0.08:0.06:1.

5. The method of claim 1 , wherein said reaction proceeds until at least one of the following: 1) a hydrodynamic size of said population of MSNPs is constant or 2) a yield of said population of MSNPs is constant.

6. The method of claim 1 , wherein said method produces said population of MSNPs characterized by at least one of the following features:
a monotonic size distribution;
a size distribution having a coefficient of variation of less than 0.10;
said population of MSNPs having an average diameter ranging from 40 nm up to 100 nm; and
an average pore size ranging from 2.2 to 3.4 nm.

7. The method of claim 1 , wherein said method further comprises:
providing a plurality of lipids in a polar solvent forming a dispersion of lipid in a solvent;
introducing said population of MSNPs into said dispersion to form a dispersion containing said population of MSNPs; and
sonicating or homogenizing said dispersion containing said population of MSNPs to provide a population of MSNPs encased in a lipid bilayer.

8. The method of claim 7 , wherein said polar solvent comprises a solvent selected from the group consisting of ethanol, methanol, ethanol containing an aqueous solvent with the organic phase greater than 30%, methanol containing the aqueous solvent with an organic phase greater than 30%, pure acetone, and acetone aqueous solution with acetone concentration of 50% or greater.

9. The method of claim 7 , wherein the ratio of the population of MSNPs to the lipid ranges from 1:0.5 to 1:5 w/w.

10. The method of claim 7 , wherein said sonication proceeds at an energy and duration sufficient to provide a clear suspension of said population of MSNPs encased in said lipid bilayer.

11. The method of claim 7 , wherein:
said plurality of lipids comprise a phospholipid, cholesterol (CHOL), and an mPEG phospholipid and said lipid bilayer encapsulating said population of MSNPs comprises said phospholipid, cholesterol (CHOL), and mPEG phospholipid; or
said lipid bilayer comprises 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG (DSPE-PEG); or
said lipid bilayer comprises DPPC/Chol/DSPE-PEG or DSPC/Chol/DSPE-PEG; or said lipid bilayer comprises a phospholipid, cholesterol, and mPEG phospholipid at a ratio of 50-90 mol % phospholipid:10-50 mol % CHOL:1-10 mol % mPEG phospholipid.

12. The method of claim 7 , wherein said lipid bilayer forms a continuous uniform and intact bilayer encompassing an entire nanoparticle within the population of MSNPs.

13. The method of claim 7 , wherein said providing said population of MSNPs comprises providing said population of MSNPs loaded with a protonating agent and wherein said population of MSNPs encased in said lipid bilayer formed by said method contain said protonating agent.

14. The method of claim 13 , wherein said protonating agent is selected from the group consisting of triethylammmonium sucrose octasulfate (TEA8SOS), proton-generating dissociable salts, a trimethylammonium salt, a triethylammonium salt, an acidic buffer, a metal salt, and calcium acetate.

15. The method of claim 13 , wherein said method comprises remote loading said population of MSNPs encased in said lipid bilayer with a drug by incubating said population of MSNPs encased in said lipid bilayer containing said protonating agent with one or more drugs comprising at least one weakly basic group capable of being protonated.

16. The method of claim 15 , wherein said drug comprises one or more of the following:
at least one weakly basic group capable of being protonated, and the protonating agent

comprises at least one anionic group;
a pKa greater than 7 and less than 11;
a primary, secondary, or tertiary amine; a water solubility index of 2 to 25 mg/mL;
an octanol/water partition coefficient or log P value of −3.0 to 3.0; or
a size smaller than the average or median size of the pores of the silica nanoparticle.

17. The method of claim 16 , wherein: said drug comprises an anticancer drug; or said drug comprises irinotecan, a substantially pure D isomer of irinotecan, or a substantially pure L isomer of irinotecan; or said drug comprises one or more drugs independently selected from the group consisting of a topoisomerase inhibitor, an antitumor anthracycline antibiotic, a mitotic inhibitor, an alkaloid, an alkaline alkylating agent, a purine or pyrimidine derivative, and a protein kinase inhibitor; or said drug comprises a drug selected from the group consisting of topotecan, 10-hydroxycamptothecin, belotecan, rubitecan, vinorelbine, LAQ824, doxorubicin, mitoxantrone, vinblastine, vinorelbine, cyclophosphamide, mechlorethamine, temozolomide, 5-fluorouracil, 5′-deoxy-5-fluorouridine, gemcitabine, imatinib, osimertinib and sunitinib pazopanib, enzastaurin, vandetanib, erlotinib, dasatinib, nilotinib, abemaciclib, palbociclib, and ribociclib.

18. The method of claim 16 , wherein said drug comprises irinotecan, a pure D isomer of irinotecan, or a pure L isomer of irinotecan.

19. The method of claim 15 , wherein:
said population of MSNPs encased in said lipid bilayer have a drug loading capacity of at least 5% w/w, or at least 10% w/w, or at least 20% w/w, or at least 30% w/w, or greater than 40% w/w, or greater than 50% w/w, or greater than 60% w/w, or greater than 70% w/w, or greater than 80% w/w; and/or
said lipid bilayer comprises a hydrophobic drug that is introduced into said lipid bilayer before encapsulation of said population of MSNPs; and/or
said lipid bilayer comprises a hydrophobic drug that is introduced into said lipid bilayer before encapsulation of said population of MSNPs where said lipid bilayer comprises a hydrophobic drug selected from the group consisting of paclitaxel, ellipticine, camptothecan, SN-38, and a lipid prodrug.

20. The method of claim 7 , wherein said population of MSNPs encased in said lipid bilayer are each conjugated to a moiety selected from the group consisting of a targeting moiety, a fusogenic peptide, and a transport peptide.

Assignees

• Univ California

Inventors

• Nel Andre E
• Meng Huan
• Liu Xiangsheng

Classifications

• A61K9/5192

  Processes · CPC title

• A61K31/4745

  condensed with ring systems having nitrogen as a ring hetero atom, e.g. phenantrolines (yohimbine derivatives, vinblastine A61K31/475; ergoline derivatives A61K31/48) · CPC title

• A61K9/5115

  Inorganic compounds · CPC title

• A61P35/00 Primary

  Antineoplastic agents · CPC title

• A61K9/1277

  Preparation processes; Proliposomes · CPC title