Scaffold material for cell culture and cell culture container

Patent metadata

Field	Value
Publication number	US-12378513-B2
Application number	US-202017599349-A
Country	US
Kind code	B2
Filing date	Mar 27, 2020
Priority date	Mar 29, 2019
Publication date	Aug 5, 2025
Grant date	Aug 5, 2025

Abstract

A scaffold material for cell culture having excellent cell colonization is provided. A scaffold material for cell culture according to the present invention includes a synthetic resin having a main chain and a graft chain, wherein the graft chain has a polydentate hydrogen-bonding group represented by the following formula (1) in a side chain: *—R-(A i ) n (1) wherein R represents a group having 50 or less carbon atoms, A i represents an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, i represents a natural number, n represents an integer of two or more and four or less, and * represents a bonding position with another atom constituting the graft chain; and A i may be identical or different from each other.

First claim

The invention claimed is:

1. A scaffold material for cell culture comprising a synthetic resin having a main chain and a graft chain,
the graft chain having a polydentate hydrogen-bonding group represented by the following formula (1) in a side chain:
[Chemical 1]
*—R-(A i ) n   (1)

wherein R represents a group having 50 or less carbon atoms, A i represents an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, i represents a natural number, n represents an integer of two or more and four or less, and represents a bonding position with another atom constituting the graft chain; and A i may be identical or different from each other, and
wherein a rate of a structural unit having the polydentate hydrogen-bonding group to 100 mol % of all structural units of the synthetic resin is 1 mol % or more and 30 mol % or less.

2. The scaffold material for cell culture according to claim 1 , wherein
the polydentate hydrogen-bonding group is a polydentate hydrogen-bonding group represented by the following formula (2), the following formula (3), or the following formula (4):

wherein R 1 represents a group having a cyclic skeleton and having 50 or less carbon atoms, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other,

wherein R 1 represents a group having a cyclic skeleton and having 50 or less carbon atoms, R 2 represents a boron atom, a phosphorus atom, a silicon atom, a nitrogen atom, or a metal atom, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other,

wherein R 3 represents a group having a chain skeleton and having 50 or less carbon atoms, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other.

3. The scaffold material for cell culture according to claim 2 , wherein the polydentate hydrogen-bonding group is a polydentate hydrogen-bonding group represented by the formula (2) or the formula (3).

4. The scaffold material for cell culture according to claim 1 , wherein the polydentate hydrogen-bonding group has two or more functional groups of at least one kind selected from the group consisting of an amino group, a carboxyl group, and a hydroxyl group.

5. The scaffold material for cell culture according to claim 1 , wherein the polydentate hydrogen-bonding group has at least one skeleton selected from the group consisting of a catechol skeleton, a phenylenediamine skeleton, a phenylboronic acid skeleton, a naphthaleneboronic acid skeleton, a glycerol skeleton, an ethylene glycol skeleton, and a glycine derivative skeleton.

6. The scaffold material for cell culture according to claim 1 , which does not substantially contain an animal-derived raw material.

7. The scaffold material for cell culture according to claim 1 , wherein the synthetic resin is a polyvinyl alcohol derivative or a poly(meth)acrylic ester derivative.

8. The scaffold material for cell culture according to claim 1 , wherein the synthetic resin has a polyvinyl acetal skeleton or a poly(meth)acrylic ester skeleton.

9. The scaffold material for cell culture according to claim 1 , wherein the synthetic resin has a polyvinyl acetal skeleton.

10. A cell culture container comprising:
a container body; and
the scaffold material for cell culture according to claim 1 ,
the scaffold material for cell culture being disposed on a surface of the container body.

11. A scaffold material for cell culture comprising a synthetic resin having a main chain and a graft chain,
the graft chain having a polydentate hydrogen-bonding group represented by the following formula (1) in a side chain:
[Chemical 1]
*—R-(A i ) n   (1)

wherein R represents a group having 50 or less carbon atoms, A i represents an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, i represents a natural number, n represents an integer of two or more and four or less, and * represents a bonding position with another atom constituting the graft chain; and A i may be identical or different from each other, and
wherein the polydentate hydrogen-bonding group has at least one skeleton selected from the group consisting of a catechol skeleton, a phenylenediamine skeleton, a phenylboronic acid skeleton, a naphthaleneboronic acid skeleton, a glycerol skeleton, an ethylene glycol skeleton, and a glycine derivative skeleton.

12. The scaffold material for cell culture according to claim 11 , wherein
the polydentate hydrogen-bonding group is a polydentate hydrogen-bonding group represented by the following formula (2), the following formula (3), or the following formula (4):

wherein R 1 represents a group having a cyclic skeleton and having 50 or less carbon atoms, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other,

wherein R 1 represents a group having a cyclic skeleton and having 50 or less carbon atoms, R 2 represents a boron atom, a phosphorus atom, a silicon atom, a nitrogen atom, or a metal atom, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other,

wherein R 3 represents a group having a chain skeleton and having 50 or less carbon atoms, A 1 and A 2 represent an amino group, a carboxyl group, a hydroxyl group, a sulfone group, an imino group, or a thiol group, respectively, and * represents a bonding site with another atom constituting the graft chain; and A 1 and A 2 may be identical or different from each other.

13. The scaffold material for cell culture according to claim 12 , wherein the polydentate hydrogen-bonding group is a polydentate hydrogen-bonding group represented by the formula (2) or the formula (3).

14. The scaffold material for cell culture according to claim 11 , wherein the polydentate hydrogen-bonding group has two or more functional groups of at least one kind selected from the group consisting of an amino group, a carboxyl group, and a hydroxyl group.

15. The sca

Assignees

• Sekisui Chemical Co Ltd

Inventors

• Iguchi Hiroki
• Nakamura Yuuta
• Ishii Ryoma
• Arai Yuuhei
• Haneda Satoshi

Classifications

• C12N2533/40

  Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12N2533/00

  Supports or coatings for cell culture, characterised by material · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12M25/06

  Plates; Walls; Drawers; Multilayer plates · CPC title