Determination of Parkinson's disease

Patent metadata

Field	Value
Publication number	US-12372537-B2
Application number	US-201917265154-A
Country	US
Kind code	B2
Filing date	Aug 8, 2019
Priority date	Aug 9, 2018
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

Methods and compositions for accurate identification of Parkinson's disease are disclosed. More particularly, the disclosure is directed to the determination of Parkinson's disease in ante-mortem tissue samples.

First claim

What is claimed is:

1. A method for determining whether a subject has Parkinson's Disease (PD), the method comprising:
(a) contacting a biological sample comprising at least one nerve feature from the subject with a primary antibody capable of binding phosphorylated alpha-synuclein; wherein the primary antibody comprises complementarity determining regions (CDRs) having the following amino acid sequences:

CDR1 VL

(SEQ ID NO: 2)

QSVYNNNN,

CDR2 VL

(SEQ ID NO: 3)

KASKVAS,

CDR3 VL

(SEQ ID NO: 4)

LGGYSGDIYT,

CDR4 VH

(SEQ ID NO: 6)

GFTISSYHMS,

CDR5 VH

(SEQ ID NO: 7)

ISTSGNI,

and

CDR6 VH 

(SEQ ID NO: 8)

ARLGIATGYSF;

(b) detecting whether the primary antibody capable of binding phosphorylated alpha-synuclein localizes within the nerve feature of the biological sample; and
(c) determining the subject has PD when the primary antibody capable of binding phosphorylated alpha-synuclein localizes within the nerve feature.

2. The method of claim 1 , wherein the sample comprises a tissue sample.

3. The method of claim 1 , wherein the nerve feature comprises:
(a) a nerve cell;
(b) a former nerve cell; or
(c) is adjacent to a nerve cell.

4. The method of claim 1 , wherein the sample is contacted with at least one protease before being contacted with the primary antibody capable of binding phosphorylated alpha-synuclein.

5. The method of claim 4 , further comprising contacting the sample with at least one phosphatase.

6. The method of claim 1 , further comprising contacting the biological sample from the subject with a primary antibody capable of binding the nerve feature.

7. The method of claim 6 , wherein the primary antibody capable of binding to the nerve feature is selected from an antibody capable of binding a protein selected from the group consisting of: ubiquitin C-terminal hydrolase L1, RNA binding fox-1 homolog 3, microtubule associated protein 2, 160 kDa neurofilament medium 200 kDa neurofilament heavy, synaptophysin, and discs large MAGUK scaffold protein 4.

8. The method of claim 1 , wherein the detecting comprises histochemical analysis.

9. The method of claim 1 , wherein the sample is fixed.

10. The method of claim 9 , wherein the sample is a formalin fixed, paraffin embedded (FFPE) sample.

11. The method of claim 1 , wherein the sample is a frozen sample.

12. The method of claim 1 , wherein the sample comprises a section of the nerve feature.

13. The method of claim 1 , wherein the sample is selected from the group consisting of skin tissue, colon tissue, and submandibular gland.

14. The method of claim 6 , wherein the primary antibody capable of binding phosphorylated alpha-synuclein and the primary antibody capable of binding to the nerve feature are from the same host species, wherein the host species is a mouse or a rabbit.

15. The method of claim 1 , wherein step (a) further comprises contacting the sample with a first secondary antibody having a first label conjugated thereto, wherein the first secondary antibody is immunoreactive with the primary antibody capable of binding phosphorylated alpha-synuclein.

16. The method of claim 15 , comprising contacting the sample with a set of reagents reactive with the first label of the first secondary antibody to generate a first detectable signal in proximity to phosphorylated alpha-synuclein in the sample.

17. The method of claim 6 , wherein before contacting the sample with the primary antibody capable of binding to the nerve feature, the method comprises denaturing the immunocomplexes in the sample.

18. The method of claim 17 , wherein the method further comprises contacting the sample with a second secondary antibody having a second label conjugated thereto, wherein the second secondary antibody is immunoreactive with the primary antibody capable of binding the nerve feature.

19. The method of claim 18 , comprising contacting the sample with a set of reagents reactive with the second label of the second secondary antibody to generate a second detectable signal in proximity to the nerve feature in the sample.

20. The method of claim 19 , wherein the first detectable signal and the second detectable signal are different.

21. The method of claim 20 , wherein the first detectable signal is silver stain.

22. The method of claim 1 , wherein the subject is suspected of having PD.

23. The method of claim 1 , wherein the antibody capable of binding phosphorylated alpha-synuclein comprises a light chain comprising SEQ ID NO: 01 and the heavy chain comprising SEQ ID NO: 05.

24. The method of claim 7 , wherein the primary antibody capable of binding the nerve feature is one of monoclonal antibody clone EPR4118 from Abcam, monoclonal antibody clone 13C/13C4 from Abcam, or polyclonal antibody from Cell Marque™ with RTD PIN 760-4434.

25. The method of claim 24 , wherein the primary antibody capable of binding the nerve feature is monoclonal antibody clone EPR4118 from Abcam (PIN ab108986).

26. A kit comprising:
(a) a primary antibody capable of binding phosphorylated alpha-synuclein wherein the primary antibody comprises complementarity determining regions (CDRs) having the following amino acid sequences:

CDR1 VL

(SEQ ID NO: 2)

QSVYNNNN,

Assignees

• Hoffmann La Roche
• Roche Diagnostics Operations Inc
• Ventana Med Syst Inc
• Prothena Biosciences Ltd

Inventors

• Czech Christian
• Dziadek Sebastian
• Pestic-Dragovich Lidija
• Tang Lei
• Kremer Thomas
• Zago Wagner Marcelo
• Tsao Tsu-Shuen
• Racolta Adriana
• Canamero Marta
• Torres Ronald
• Ritter Mirko

Classifications

• G01N2800/2835

  Movement disorders, e.g. Parkinson, Huntington, Tourette · CPC title

• C07K2317/33

  Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity · CPC title

• C07K2317/94

  Stability, e.g. half-life, pH, temperature or enzyme-resistance · CPC title

• C07K16/18

  against material from animals or humans · CPC title

• G01N2474/20

  Immunohistochemistry assay · CPC title