Maize event DP-915635-4 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-12371707-B2
Application number	US-202117332245-A
Country	US
Kind code	B2
Filing date	May 27, 2021
Priority date	Jun 3, 2020
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-915635-4, and to assays for detecting the presence of event DP-915635-4 in samples and compositions thereof.

First claim

What is claimed is:

1. A corn plant comprising event DP-915635-4, wherein said event comprises a nucleotide sequence as set forth in SEQ ID NO: 26 and SEQ ID NO: 29, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746.

2. The corn plant of claim 1 , wherein said event comprises the nucleotide sequence set forth in SEQ ID NO: 27 and SEQ ID NO: 30.

3. The corn plant of claim 1 , wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 28 and SEQ ID NO: 31.

4. A DNA construct comprising an operably linked first and second expression cassette, wherein said first expression cassette comprises:
1) an sb-RCc3 Enhancer
2) a zm-PCOa Promoter;
3) a zm-HPLV9 Intron;
4) an ipd079Ea; and
5) an sb-SCI-1B Terminator.

5. A plant comprising the DNA construct of claim 4 .

6. The plant of claim 5 , wherein said plant is a corn plant.

7. A plant comprising the sequence set forth in SEQ ID NO: 21.

8. A corn event DP-915635-4, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746.

9. Plant parts of the corn event of claim 8 , wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746, wherein the plant parts comprise the corn event DP-915635-4.

10. A corn seed comprising corn event DP-915635-4, wherein said seed comprises a DNA molecule chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein a representative sample of the corn event DP-915635-4 seed of has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746.

11. A corn plant, or part thereof, grown from the seed of claim 10 .

12. A transgenic seed produced from the corn event of claim 8 .

13. A transgenic corn plant, or part thereof, grown from the seed of claim 12 .

14. An isolated nucleic acid molecule comprising a nucleotide sequence chosen from SEQ ID NOs: 21, 26-28, and 31, and full length complements thereof.

15. An amplicon comprising the nucleic acid sequence chosen from SEQ ID NOs: 22 and 23 and full length complements thereof.

16. A biological sample derived from corn event DP-915635-4 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-915635-4 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746.

17. The biological sample of claim 16 , wherein said biological sample comprises plant, plant tissue, or seed of transgenic corn event DP-915635-4.

18. The biological sample of claim 17 , wherein said biological sample is a DNA sample extracted from the transgenic corn plant event DP-915635-4, and wherein said DNA sample comprises one or more of the nucleotide sequences chosen from SEQ ID NOs: 21-31, and the complement thereof.

19. The biological sample of claim 16 , wherein said biological sample is chosen from corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products.

20. An extract derived from corn event DP-915635-4 plant, tissue, or seed and comprising a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 26 and SEQ ID NO: 29, wherein a representative sample of said corn event DP-915635-4 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746.

21. The extract of claim 20 , wherein said nucleotide sequence is detectable in said extract using a nucleic acid amplification or nucleic acid hybridization method.

22. The extract of claim 21 , wherein said extract comprises plant, plant tissue, or seed of transgenic corn plant event DP-915635-4.

23. The extract of claim 22 , wherein the extract is a composition chosen from corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products, wherein said composition comprises a detectable amount of said nucleotide sequence.

24. A method of producing hybrid corn seeds comprising:
A) sexually crossing a first inbred corn line comprising a nucleotide chosen from SEQ ID NOs: 21, 22, 24, 26-31 and a second inbred line having a different genotype;
B) growing progeny from said crossing; and
C) harvesting the hybrid seed produced thereby.

25. The method according to claim 24 , wherein the first inbred corn line is a female parent.

26. The method according to claim 24 , wherein the first inbred corn line is a male parent.

27. A method for producing a corn plant resistant to coleopteran pests comprising:
A) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises event DP-915635-4, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746, thereby producing a plurality of first generation progeny plants;
B) selfing the first generation progeny plant, thereby producing a plurality of second generation progeny plants; and
C) selecting from the second generation progeny plants that comprise the event DP-915635-4 and are resistant to a coleopteran pest.

28. A method of producing hybrid corn seeds comprising:
A) sexually crossing a first inbred corn line comprising the DNA construct of claim 1 with a second inbred line not comprising the DNA construct of claim 1 ; and
B) harvesting the hybrid seed produced thereby.

29. The method of claim 28 , further comprising the step of backcrossing a second generation progeny plant that comprises corn event DP-915635-4 to the parent plant that lacks the corn event DP-915635-4 DNA, thereby producing a backcross progeny plant that is resistant to a coleopteran pest.

30. A method for producing a corn plant resistant to a corn rootworm, said method comprising:
A) crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises event DP-915635-4, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-126746, thereby producing a plurality of first generation progeny plants;
B) selecting a first generation progeny plant that comprises the event DP-915635-4;
C) backcrossing the first generation progeny plant of step (b) with a parent plant that lacks the corn event DP-915635-4 DNA, thereby producing a plurality of backcross progeny plants; and
D) selecting from the backcross progeny plants, a plant that comprises the event DP-915635-4;
wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO: 21, 26, or 29.

31. The method according to claim 30 , wherein the plants of the first parent corn line are the female parents or male parents.

32. Hybrid seed produced by the method of claim 30 .

33. A method of determining zygosi

Assignees

• Pioneer Hi Bred Int

Inventors

• Christensen Heather Marie
• Cong Bin
• Crane Virginia
• Harmon Matthew Curtis
• Jaureguy Luciano M
• Klever Jeffrey
• Lu Albert L
• Rinehart Krebs Kristen Denise
• Ross Margit C

Classifications

• A01H5/10

  Seeds · CPC title

• A01H1/127

  for insect resistance · CPC title

• A01H6/4684 Primary

  Zea mays [maize] · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title