Method for the preparation of lysophosphatidylinositol

Patent metadata

Field	Value
Publication number	US-12371449-B2
Application number	US-202117235561-A
Country	US
Kind code	B2
Filing date	Apr 20, 2021
Priority date	Jul 6, 2020
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

The present invention relates to a high-LPI lysolecithin and methods related to the same. The high-LPI product is produced through the reaction of lecithin with a unique method using solvent, buffer/water, and phospholipase. Using the current production method, LPI content increased from 1.4% to 3.2-13.1% using regular soy lecithin as the starting material and LPC, LPE and LPA contents also increased from 5.1%, 2.0%, 1.0% to 15.8%, 14.6% and 4.4% respectively.

First claim

The invention claimed is:

1. A method of producing high-LPI lysolecithin wherein resulting lysolecithin comprises at least 2.5% by weight LPI, wherein the method comprises the steps of:
mixing a source of lecithin with an organic solvent, at least one buffer or water, and a phospholipase to form a reaction mixture;
heating the reaction mixture to 40 to 65° C. for about 0.5 to 5 hours;
cooling the reaction mixture to 40° C. or below to separate the high-LPI lysolecithin from the reaction mixture; and
collecting the high-LPI lysolecithin.

2. The method of claim 1 further comprising the step of drying the high-LPI lysolecithin to a moisture content of 10% or less.

3. The method of claim 1 wherein the organic solvent is an acetate or an ether.

4. The method of claim 1 wherein the organic solvent is selected from the group consisting of ethyl acetate (EtOAc), methyl tert-butyl ether and isopropyl acetate (iPrOAc).

5. The method of claim 1 wherein the phospholipase is phospholipase A1 (PLA1).

6. The method of claim 1 wherein the at least one buffer is a phosphate buffer.

7. The method of claim 6 wherein the phosphate buffer is phosphate buffered solution (PBS).

8. The method of claim 1 wherein the organic solvent is included in an amount of at least about 100% volume of organic solvent to lecithin weight.

9. The method of claim 7 wherein the organic solvent is included in an amount of between 300-600% volume of solvent to lecithin weight.

10. The method of claim 1 wherein the at least one buffer or water is included in an amount of at least about 1% by weight buffer or water to lecithin weight.

11. The method of claim 10 wherein the buffer or water is included in an amount of about 20-90% weight of buffer or water to lecithin weight being preferred.

12. The method of claim 5 wherein the PLA1 is included in an amount of at least about 0.1% by weight PLA1 to lecithin weight.

13. The method of claim 12 wherein the PLA1 is included in an amount of about 0.2-0.5% by weight PLA1 to lecithin weight being preferred.

14. The method of claim 1 wherein the mixture is heated to a temperature sufficient to cause hydrolysis of the lecithin to form a heated mixture.

15. The method of claim 14 wherein the mixture is heated to a temperature ranging from about 40-65° C. for a time period of about 0.5-5 hours.

16. The method of claim 14 further including the step of cooling the heated mixture for a period of time sufficient to allow lysolecithin to separate from the mixture.

17. The method of claim 16 further including the steps of collecting the lysolecithin and drying the lysolecithin.

18. The method of claim 17 wherein the lysolecithin is dried to a moisture content of about 10% by weight or less.

19. The method of claim 1 wherein the resulting lysolecithin comprise at least 0.0% and 2.0% by weight LPC and LPA, respectively.

20. The method of claim 3 , wherein the organic solvent is ethyl acetate.

21. The method of claim 16 , wherein the mixture is cooled to a temperature of 40° C. or less for a time period of at least 30 minutes.

22. The method of claim 1 , wherein a lysophosphatidylinositol (LPI) content is increased from 1.4% to 6.5%.

Assignees

• Kemin Ind Inc

Inventors

• Li Zheng
• Li Songying
• Huang Shengshu

Classifications

• C12P7/6436

  Fatty acid esters · CPC title

• C07F9/117

  with cycloaliphatic alcohols · CPC title

• C07F9/103 Primary

  Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure · CPC title